Is plasma {beta}-glucuronidase a novel human biomarker for monitoring anticholinesterase pesticides exposure? A Malaysian experience

Journal Article · Thu Mar 15 00:00:00 EDT 2007 · Toxicology and Applied Pharmacology

DOI:https://doi.org/10.1016/j.taap.2006.10.014· OSTI ID:20976887

Inayat-Hussain, Salmaan H ^[1]; Lubis, Syarif Husin ^[2]; Sakian, Noor Ibrahim Mohamed; Ghazali, Ahmad Rohi; Ali, Noor Suhailah ^[3]; El Sersi, Magdi; Toong, Lee Mun; Zainal, Awang Mat; Hashim, Suhaimi; Ghazali, Mohd Shariman ^[2]; Saidin, Mohd Nazri ^[2]; Rahman, Ab Razak Ab ^[3]; Rafaai, Mohd Jamil Mohd ^[3]; Omar, Sollahudin ^[3]; Rapiai, Rafiah; Othman, Radziah ^[3]; Chan, Lee Tiong; Johari, Amran ^[4]; Soon, Wong Hing; Salleh, Abdul Rahim ^[5] ; Satoh, Tetsuo ^[6]

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1. Melaka Institute of Biotechnology, Lot 7, MITC City, 75450 Ayer Keroh, Melaka (Malaysia)
2. Kulliyah of Medicine, International Islamic University of Malaysia, Jalan Istana, Bandar Indera Mahkota, 25200 Kuantan, Pahang (Malaysia)
3. Faculty of Allied Health Sciences, Universiti Kebangsaan Malaysia, Jalan Raja Muda Abdul Aziz, 50300 Kuala Lumpur (Malaysia)
4. Tengku Ampuan Rahimah Hospital, Klang, Jalan Langat 41200 Klang, Selangor (Malaysia)
5. Muda Agriculture Development Authority (MADA), Ampang Jajar, 50990 Alor Setar, Kedah (Malaysia)
6. Graduate School of Pharmaceutical Sciences, Chiba University, Chiba 260-8675 (Japan)

A cross-sectional study was conducted to investigate the effects of acute and chronic pesticide exposure on the plasma {beta}-glucuronidase enzyme activity among five patients of acute pesticide poisoning in Tengku Ampuan Rahimah Hospital, Klang, 230 farmers in the MADA area, Kedah and 49 fishermen in Setiu, Terengganu. The duration of pesticide exposure among the patients was unknown, but the plasma samples from patients were collected on day one in the hospital. The duration of pesticide exposure among the farmers was between 1 and 45 years. The {beta}-glucuronidase activity was compared with plasma cholinesterase activity in the same individual. The plasma cholinesterase activity was measured using Cholinesterase (PTC) Reagent set kit (Teco Diagnostics, UK) based on colorimetric method, while the plasma {beta}-glucuronidase activity was measured fluorometrically based on {beta}-glucuronidase assay. The plasma cholinesterase activity was significantly reduced (p < 0.05) among the patients (1386.786 {+-} 791.291 U/L/min) but the inhibition in plasma cholinesterase activity among the farmers (7346.5 {+-} 1860.786 U/L/min) was not significant (p > 0.05). The plasma {beta}-glucuronidase activity among the farmers was significantly elevated (p < 0.05) (0.737 {+-} 0.425 {mu}M/h) but not significant among the patients (p > 0.05). The plasma cholinesterase activity was positively correlated with the plasma {beta}-glucuronidase activity among the farmers (r = 0.205, p < 0.01) but not among the patients (r = 0.79, p > 0.05). Thus, plasma {beta}-glucuronidase enzyme activity can be measured as a biomarker for the chronic exposure of pesticide. However, further studies need to be performed to confirm whether plasma {beta}-glucuronidase can be a sensitive biomarker for anticholinesterase pesticide poisoning.

• OSTI ID: 20976887
• Journal Information: Toxicology and Applied Pharmacology, Vol. 219, Issue 2-3; Other Information: DOI: 10.1016/j.taap.2006.10.014; PII: S0041-008X(06)00374-7; Copyright (c) 2006 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved; Country of input: International Atomic Energy Agency (IAEA); ISSN 0041-008X
• Country of Publication: United States
• Language: English

Related Subjects

60 APPLIED LIFE SCIENCES
BIOLOGICAL MARKERS
BLOOD PLASMA
CHOLINESTERASE
CHRONIC EXPOSURE
ENZYME ACTIVITY
GLUCURONIDASE
HOSPITALS
PATIENTS
PESTICIDES
POISONING
SULFUR 49