Unique CCT repeats mediate transcription of the TWIST1 gene in mesenchymal cell lines
- Maxillofacial Orthognathics, Graduate School, Tokyo Medical and Dental University, Tokyo 113-8549 (Japan)
- Human Gene Sciences Center, Tokyo Medical and Dental University, Tokyo 113-8510 (Japan)
TWIST1, a basic helix-loop-helix transcription factor, plays critical roles in embryo development, cancer metastasis and mesenchymal progenitor differentiation. Little is known about transcriptional regulation of TWIST1 expression. Here we identified DNA sequences responsible for TWIST1 expression in mesenchymal lineage cell lines. Reporter assays with TWIST1 promoter mutants defined the -102 to -74 sequences that are essential for TWIST1 expression in human and mouse mesenchymal cell lines. Tandem repeats of CCT, but not putative CREB and NF-{kappa}B sites in the sequences substantially supported activity of the TWIST1 promoter. Electrophoretic mobility shift assay demonstrated that the DNA sequences with the CCT repeats formed complexes with nuclear factors, containing, at least, Sp1 and Sp3. These results suggest critical implication of the CCT repeats in association with Sp1 and Sp3 factors in sustaining expression of the TWIST1 gene in mesenchymal cells.
- OSTI ID:
- 20857974
- Journal Information:
- Biochemical and Biophysical Research Communications, Vol. 352, Issue 4; Other Information: DOI: 10.1016/j.bbrc.2006.11.114; PII: S0006-291X(06)02608-8; Copyright (c) 2006 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved; Country of input: International Atomic Energy Agency (IAEA); ISSN 0006-291X
- Country of Publication:
- United States
- Language:
- English
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