Hypo-osmotic shock induces nuclear export and proteasome-dependent decrease of UBL5
- Mitsubishi Kagaku Institute of Life Sciences (MITILS), Machida, Tokyo 194-8511 (Japan)
- Mitsubishi Kagaku Institute of Life Sciences (MITILS), Machida, Tokyo 194-8511 (Japan) and National Institutes of Natural Sciences, National Institute for Physiological Sciences, 5-1 Higashiyama, Myodaiji-cho, Okazaki, Aichi 444-8787 (Japan)
The osmolarity of body fluid is strictly controlled through the action of diuretic hormones, which are secreted in the hypothalamus. In the mammalian brain, ubiquitin-like 5 (UBL5) is expressed in oxytocin- and vasopressin-positive neurons in the hypothalamus, and these neurons play a role in regulating osmolarity. We examined the dynamics of UBL5 levels in response to hyper- or hypo-osmotic conditions. Hypo-osmotic conditions led to significantly reduced levels of UBL5 both in brain slices from the hypothalamus and in NIH-3T3 cells. This decrease in UBL5 was transcription-independent and proteasome-dependent. Time-course immunocytochemical studies using exogenous UBL5 revealed that the protein was exported from the nucleus under hypo-osmotic conditions and decreased in a proteasome-dependent manner. This report is the first to describe changes in the intracellular and subcellular localization of UBL5 in response to hypo-osmotic conditions. Our results imply osmoregulation of UBL5.
- OSTI ID:
- 20854583
- Journal Information:
- Biochemical and Biophysical Research Communications, Vol. 350, Issue 3; Other Information: DOI: 10.1016/j.bbrc.2006.09.093; PII: S0006-291X(06)02125-5; Copyright (c) 2006 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved; Country of input: International Atomic Energy Agency (IAEA); ISSN 0006-291X
- Country of Publication:
- United States
- Language:
- English
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