Effects of ghrelin and des-acyl ghrelin on neurogenesis of the rat fetal spinal cord

Journal Article · Fri Nov 24 00:00:00 EST 2006 · Biochemical and Biophysical Research Communications

DOI:https://doi.org/10.1016/j.bbrc.2006.09.088· OSTI ID:20854581

Sato, Miho ^[1]; Nakahara, Keiko ^[1]; Goto, Shintaro ^[1]; Kaiya, Hiroyuki ^[2]; Miyazato, Mikiya ^[2]; Date, Yukari ^[3]; Nakazato, Masamitsu ^[3]; Kangawa, Kenji ^[2]; Murakami, Noboru ^[1]

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1. Department of Veterinary Physiology, Faculty of Agriculture, University of Miyazaki, Miyazaki 889-2155 (Japan)
2. National Cardiovascular Center Research Institute, Osaka 565-8565 (Japan)
3. Third Department of Internal Medicine, University of Miyazaki, Kiyotake, Miyazaki 889-1692 (Japan)

Expressions of the growth hormone secretagogue receptor (GHS-R) mRNA and its protein were confirmed in rat fetal spinal cord tissues by RT-PCR and immunohistochemistry. In vitro, over 3 nM ghrelin and des-acyl ghrelin induced significant proliferation of primary cultured cells from the fetal spinal cord. The proliferating cells were then double-stained using antibodies against the neuronal precursor marker, nestin, and the cell proliferation marker, 5-bromo-2'-deoxyuridine (BrdU), and the nestin-positive cells were also found to be co-stained with antibody against GHS-R. Furthermore, binding studies using [{sup 125}I]des-acyl ghrelin indicated the presence of a specific binding site for des-acyl ghrelin, and confirmed that the binding was displaced with unlabeled des-acyl ghrelin or ghrelin. These results indicate that ghrelin and des-acyl ghrelin induce proliferation of neuronal precursor cells that is both dependent and independent of GHS-R, suggesting that both ghrelin and des-acyl ghrelin are involved in neurogenesis of the fetal spinal cord.

• OSTI ID: 20854581
• Journal Information: Biochemical and Biophysical Research Communications, Vol. 350, Issue 3; Other Information: DOI: 10.1016/j.bbrc.2006.09.088; PII: S0006-291X(06)02123-1; Copyright (c) 2006 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved; Country of input: International Atomic Energy Agency (IAEA); ISSN 0006-291X
• Country of Publication: United States
• Language: English

Related Subjects

60 APPLIED LIFE SCIENCES
ANTIBODIES
CELL PROLIFERATION
DEOXYURIDINE
IN VITRO
IODINE 125
POLYMERASE CHAIN REACTION
RATS
RECEPTORS
SPINAL CORD
STH