Ten tandem repeats of {beta}-hCG 109-118 enhance immunogenicity and anti-tumor effects of {beta}-hCG C-terminal peptide carried by mycobacterial heat-shock protein HSP65

Journal Article · Fri Jul 14 00:00:00 EDT 2006 · Biochemical and Biophysical Research Communications

DOI:https://doi.org/10.1016/j.bbrc.2006.05.022· OSTI ID:20854353

Yankai, Zhang ^[1]; Rong, Yan ^[1]; Yi, He ^[1]; Wentao, Liu ^[2]; Rongyue, Cao ^[1]; Ming, Yan ^[3]; Taiming, Li ^[1]; Jingjing, Liu ^[1]; Jie, Wu ^[1]

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1. Minigene Pharmacy Laboratory, Biopharmaceutical College, China Pharmaceutical University, Tongjia Xiang 24, Nanjing 210009 (China)
2. Nanjing Medical University, Nanjing 210029 (China)
3. National Drug Screening Laboratory, China Pharmaceutical University, Tongjia Xiang 24, Nanjing 210009 (China)

The {beta}-subunit of human chorionic gonadotropin ({beta}-hCG) is secreted by many kinds of tumors and it has been used as an ideal target antigen to develop vaccines against tumors. In view of the low immunogenicity of this self-peptide,we designed a method based on isocaudamer technique to repeat tandemly the 10-residue sequence X of {beta}-hCG (109-118), then 10 tandemly repeated copies of the 10-residue sequence combined with {beta}-hCG C-terminal 37 peptides were fused to mycobacterial heat-shock protein 65 to construct a fusion protein HSP65-X10-{beta}hCGCTP37 as an immunogen. In this study, we examined the effect of the tandem repeats of this 10-residue sequence in eliciting an immune by comparing the immunogenicity and anti-tumor effects of the two immunogens, HSP65-X10-{beta}hCGCTP37 and HSP65-{beta}hCGCTP37 (without the 10 tandem repeats). Immunization of mice with the fusion protein HSP65-X10-{beta}hCGCTP37 elicited much higher levels of specific anti-{beta}-hCG antibodies and more effectively inhibited the growth of Lewis lung carcinoma (LLC) in vivo than with HSP65-{beta}hCGCTP37, which should suggest that HSP65-X10-{beta}hCGCTP37 may be an effective protein vaccine for the treatment of {beta}-hCG-dependent tumors and multiple tandem repeats of a certain epitope are an efficient method to overcome the low immunogenicity of self-peptide antigens.

• OSTI ID: 20854353
• Journal Information: Biochemical and Biophysical Research Communications, Vol. 345, Issue 4; Other Information: DOI: 10.1016/j.bbrc.2006.05.022; PII: S0006-291X(06)01045-X; Copyright (c) 2006 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved; Country of input: International Atomic Energy Agency (IAEA); ISSN 0006-291X
• Country of Publication: United States
• Language: English

Related Subjects

60 APPLIED LIFE SCIENCES
ANTIBODIES
ANTIGENS
CARCINOMAS
HCG
HEAT-SHOCK PROTEINS
IMMUNOTHERAPY
IN VIVO
LUNGS
MICE
PEPTIDES
VACCINES