UVA-mediated down-regulation of MMP-2 and MT1-MMP coincides with impaired angiogenic phenotype of human dermal endothelial cells
- CNRS UMR 6198, IFR 53 Biomolecules, Faculty of Medicine, 51, rue Cognacq Jay, Reims 51100 (France)
- EA 3796, IFR53 'Biomolecules', Faculty of Medicine, Reims (France)
- CNRS UMR 6142, IFR53 'Biomolecules', Faculty of Medicine, Reims (France)
- EA2087, North Hospital, Amiens (France)
UVA irradiation, dose-dependently (5-20 J/cm{sup 2}), was shown to impair the morphogenic differentiation of human microvascular endothelial cells (HMECs) on Matrigel. Parallely, UVA down-regulated the expression of MMP-2 and MT1-MMP, both at the protein and the mRNA levels. On the contrary, the production of MMP-1 and TIMP-1 by HMECs increased following UVA treatment. The inhibitory effect of UVA on MMP expression and pseudotubes formation was mediated by UVA-generated singlet oxygen ({sup 1}O{sub 2}). The contribution of MT1-MMP, but not TIMP-1, to the regulation of HMECs' angiogenic phenotype following UVA irradiation was suggested using elastin-derived peptides and TIMP-1 blocking antibody, respectively.
- OSTI ID:
- 20854327
- Journal Information:
- Biochemical and Biophysical Research Communications, Vol. 345, Issue 2; Other Information: DOI: 10.1016/j.bbrc.2006.04.115; PII: S0006-291X(06)00856-4; Copyright (c) 2006 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved; Country of input: International Atomic Energy Agency (IAEA); ISSN 0006-291X
- Country of Publication:
- United States
- Language:
- English
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