Involvement of the GC-rich sequence and specific proteins (Sp1/Sp3) in the basal transcription activity of neurogranin gene
- Department of Biological Sciences, National University of Singapore, 14 Science Drive 4, Singapore 117543 (Singapore)
- Department of Pharmacy, National University of Singapore, 18 Science Drive 4, Singapore 117543 (Singapore)
- Department of Biological Sciences, National University of Singapore, 14 Science Drive 4, Singapore 117543 (Singapore) and University Scholars Program, National University of Singapore, 10 Kent Ridge Crescent, Singapore 119260 (Singapore)
Neurogranin (Ng), a neuronal protein implicated in learning and memory, contains a TATA-less promoter. Analysis of 5'-deletion mutations and site-directed mutations of the mouse Ng promoter revealed that a 258 bp 5'-flanking sequence (+3 to +260) conferred the basal transcription activity, and that the GC-rich sequence (+22 to +33) served as an important determinant of the promoter activity. Transient transfection of the Sp1 expression plasmid transactivated the reporter activity in neuroblastoma N2A cells while knocking down of endogenous Sp1 expression resulted in a 2.5-fold reduction of the reporter activity in HEK 293 cells. Exogenous expression of Sp3 in HEK 293 cells, however, repressed the reporter activity by 50%. Nevertheless, by gel shift assays, Sp1 and Sp3 were not found to be responsible for the protein-DNA complexes formed by the GC-rich sequence. Moreover, a nuclear factor from the mouse brain tissues was discovered to bind to multiple AT-rich regions in Ng promoter.
- OSTI ID:
- 20854308
- Journal Information:
- Biochemical and Biophysical Research Communications, Vol. 345, Issue 1; Other Information: DOI: 10.1016/j.bbrc.2006.04.054; PII: S0006-291X(06)00865-5; Copyright (c) 2006 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved; Country of input: International Atomic Energy Agency (IAEA); ISSN 0006-291X
- Country of Publication:
- United States
- Language:
- English
Similar Records
Genetic Selection for Context-Dependent Stochastic Phenotypes: Sp1 and TATA Mutations Increase Phenotypic Noise in HIV-1 Gene Expression
Cyclin A regulates a cell-cycle-dependent expression of CKAP2 through phosphorylation of Sp1