Caught in the act: In vivo molecular imaging of the transcription factor NF-{kappa}B after myocardial infarction

Journal Article · Fri Apr 14 00:00:00 EDT 2006 · Biochemical and Biophysical Research Communications

DOI:https://doi.org/10.1016/j.bbrc.2006.02.024· OSTI ID:20798891

Tillmanns, Jochen ^[1]; Carlsen, Harald ^[2]; Blomhoff, Rune ^[2]; Valen, Guro ^[2]; Calvillo, Laura ^[1]; Ertl, Georg ^[1]; Bauersachs, Johann ^[1]; Frantz, Stefan ^[1]

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1. Medizinische Klinik der Universitaet Wuerzburg (Germany)
2. University of Oslo, Oslo (Norway)

Nuclear factor {kappa}B (NF-{kappa}B) is a ubiquitous transcription factor activated by various stimuli implicated in heart failure progression. However, its activation in heart failure has not been well defined yet. Therefore, we investigated activation of NF-{kappa}B after myocardial infarction. For First time, we performed serial, non-invasive in vivo molecular imaging of transcription factor activation in the heart. We used mice expressing a luciferase reporter whose transcription is dependent upon NF-{kappa}B activation for up to 8 weeks after myocardial infarction. There was a significant increase of NF-{kappa}B activity with a maximum at day 3 after myocardial infarction when compared to sham controls. Thus, in vivo measurement of the activation of NF-{kappa}B is feasible. NF-{kappa}B activity might play an important role for the remodeling process.

• OSTI ID: 20798891
• Journal Information: Biochemical and Biophysical Research Communications, Vol. 342, Issue 3; Other Information: DOI: 10.1016/j.bbrc.2006.02.024; PII: S0006-291X(06)00313-5; Copyright (c) 2006 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved; Country of input: International Atomic Energy Agency (IAEA); ISSN 0006-291X
• Country of Publication: United States
• Language: English

Related Subjects

60 APPLIED LIFE SCIENCES
HEART
HEART FAILURE
IN VIVO
INFLAMMATION
LUCIFERASE
MICE
MYOCARDIAL INFARCTION
STIMULI
TRANSCRIPTION
TRANSCRIPTION FACTORS