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Title: The C-terminal tail of protein kinase D2 and protein kinase D3 regulates their intracellular distribution

Abstract

We generated a set of GFP-tagged chimeras between protein kinase D2 (PKD2) and protein kinase D3 (PKD3) to examine in live cells the contribution of their C-terminal region to their intracellular localization. We found that the catalytic domain of PKD2 and PKD3 can localize to the nucleus when expressed without other kinase domains. However, when the C-terminal tail of PKD2 was added to its catalytic domain, the nuclear localization of the resulting protein was inhibited. In contrast, the nuclear localization of the CD of PKD3 was not inhibited by its C-terminal tail. Furthermore, the exchange of the C-terminal tail of PKD2 and PKD3 in the full-length proteins was sufficient to exchange their intracellular localization. Collectively, these data demonstrate that the short C-terminal tail of these kinases plays a critical role in determining their cytoplasmic/nuclear localization.

Authors:
 [1];  [1];  [1]
  1. Unit of Signal Transduction and Gastrointestinal Cancer, Division of Digestive Diseases, Department of Medicine, CURE Digestive Diseases Research Center and Molecular Biology Institute, David Geffen School of Medicine, University of California at Los Angeles, Los Angeles (United States)
Publication Date:
OSTI Identifier:
20798886
Resource Type:
Journal Article
Journal Name:
Biochemical and Biophysical Research Communications
Additional Journal Information:
Journal Volume: 342; Journal Issue: 3; Other Information: DOI: 10.1016/j.bbrc.2006.02.013; PII: S0006-291X(06)00292-0; Copyright (c) 2006 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved; Country of input: International Atomic Energy Agency (IAEA); Journal ID: ISSN 0006-291X
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; CELL NUCLEI; CHIMERAS; GTP-ASES; PHOSPHOTRANSFERASES; RECEPTORS

Citation Formats

Papazyan, Romeo, Rozengurt, Enrique, and Rey, Osvaldo. The C-terminal tail of protein kinase D2 and protein kinase D3 regulates their intracellular distribution. United States: N. p., 2006. Web. doi:10.1016/j.bbrc.2006.02.013.
Papazyan, Romeo, Rozengurt, Enrique, & Rey, Osvaldo. The C-terminal tail of protein kinase D2 and protein kinase D3 regulates their intracellular distribution. United States. https://doi.org/10.1016/j.bbrc.2006.02.013
Papazyan, Romeo, Rozengurt, Enrique, and Rey, Osvaldo. 2006. "The C-terminal tail of protein kinase D2 and protein kinase D3 regulates their intracellular distribution". United States. https://doi.org/10.1016/j.bbrc.2006.02.013.
@article{osti_20798886,
title = {The C-terminal tail of protein kinase D2 and protein kinase D3 regulates their intracellular distribution},
author = {Papazyan, Romeo and Rozengurt, Enrique and Rey, Osvaldo},
abstractNote = {We generated a set of GFP-tagged chimeras between protein kinase D2 (PKD2) and protein kinase D3 (PKD3) to examine in live cells the contribution of their C-terminal region to their intracellular localization. We found that the catalytic domain of PKD2 and PKD3 can localize to the nucleus when expressed without other kinase domains. However, when the C-terminal tail of PKD2 was added to its catalytic domain, the nuclear localization of the resulting protein was inhibited. In contrast, the nuclear localization of the CD of PKD3 was not inhibited by its C-terminal tail. Furthermore, the exchange of the C-terminal tail of PKD2 and PKD3 in the full-length proteins was sufficient to exchange their intracellular localization. Collectively, these data demonstrate that the short C-terminal tail of these kinases plays a critical role in determining their cytoplasmic/nuclear localization.},
doi = {10.1016/j.bbrc.2006.02.013},
url = {https://www.osti.gov/biblio/20798886}, journal = {Biochemical and Biophysical Research Communications},
issn = {0006-291X},
number = 3,
volume = 342,
place = {United States},
year = {Fri Apr 14 00:00:00 EDT 2006},
month = {Fri Apr 14 00:00:00 EDT 2006}
}