Anti-allergic effects of Lycopus lucidus on mast cell-mediated allergy model
- College of Pharmacy, Woosuk University, Jeonju, Jeonbuk 565-701 (Korea, Republic of)
- Department of Pharmacology, School of Medicine, Kyungpook National University, Daegu 700-422 (Korea, Republic of)
- Department of Physiology, School of Medicine, Kyungpook National University, Daegu 700-422 (Korea, Republic of)
- Division of Specimen and Genetic Resources, Korea National Arboretum, Gyeonggi 487-821 (Korea, Republic of)
- College of Pharmacy, Wonkwang University, Jeonbuk 570-749 (Korea, Republic of)
- College of Oriental Medicine, Kyung Hee University, Seoul 130-701 (Korea, Republic of)
The current study characterizes the mechanism by which the aqueous extract of Lycopus lucidus Turcz. (Labiatae) (LAE) decreases mast cell-mediated immediate-type allergic reaction. The immediate-type allergic reaction is involved in many allergic diseases such as asthma and allergic rhinitis. LAE has been used as a traditional medicine in Korea and is known to have an anti-inflammatory effect. However, its specific mechanism of action is still unknown. LAE was anally administered to mice for high and fast absorption. LAE inhibited compound 48/80-induced systemic reactions in mice. LAE decreased the local allergic reaction, passive cutaneous anaphylaxis, activated by anti-dinitrophenyl (DNP) IgE antibody. LAE dose-dependently reduced histamine release from rat peritoneal mast cells activated by compound 48/80 or anti-DNP IgE. Furthermore, LAE decreased the secretion of TNF-{alpha} and IL-6 in phorbol 12-myristate 13-acetate (PMA) plus calcium ionophore A23187-stimulated human mast cells. The inhibitory effect of LAE on the pro-inflammatory cytokine was p38 mitogen-activated protein kinase (MAPK) and nuclear factor-{kappa}B (NF-{kappa}B) dependent. LAE attenuated PMA plus A23187-induced degradation of I{kappa}B{alpha} and nuclear translocation of NF-{kappa}B, and specifically blocked activation of p38 MAPK, but not that of c-jun N-terminal kinase and extracellular signal-regulated kinase. Our findings provide evidence that LAE inhibits mast cell-derived immediate-type allergic reactions and involvement of pro-inflammatory cytokines, p38 MAPK, and NF-{kappa}B in these effects.
- OSTI ID:
- 20783389
- Journal Information:
- Toxicology and Applied Pharmacology, Vol. 209, Issue 3; Other Information: DOI: 10.1016/j.taap.2005.04.011; PII: S0041-008X(05)00184-5; Copyright (c) 2005 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved; Country of input: International Atomic Energy Agency (IAEA); ISSN 0041-008X
- Country of Publication:
- United States
- Language:
- English
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