The PCNA-associated factor KIAA0101/p15{sup PAF} binds the potential tumor suppressor product p33ING1b

Journal Article · Sun Jan 01 00:00:00 EST 2006 · Experimental Cell Research

DOI:https://doi.org/10.1016/j.yexcr.2005.09.020· OSTI ID:20775327

Simpson, Fiona ^[1]; Lammerts van Bueren, Kelly ^[1]; Butterfield, Natalie ^[1]; Bennetts, Jennifer S ^[1]; Bowles, Josephine ^[1]; Adolphe, Christelle ^[1]; Simms, Lisa A ^[2]; Young, Joanne ^[2]; Walsh, Michael D ^[2]; Leggett, Barbara ^[2]; Fowles, Lindsay F ^[1]; Wicking, Carol ^[1]

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1. Institute for Molecular Bioscience, University of Queensland, St Lucia, Brisbane, Queensland 4072 (Australia)
2. Conjoint Gastroenterology Laboratory, Royal Brisbane and Womens Hospital Research Foundation and Queensland Institute of Medical Research, Brisbane, QLD 4029 (Australia)

The KIAA0101/p15{sup PAF}/OEATC-1 protein was initially isolated in a yeast two-hybrid screen for proliferating cell nuclear antigen (PCNA) binding partners, and was shown to bind PCNA competitively with the cell cycle regulator p21{sup WAF}. PCNA is involved in DNA replication and damage repair. Using polyclonal antisera raised against a p15{sup PAF} fusion protein, we have shown that in a range of mammalian tumor and non-tumor cell lines the endogenous p15{sup PAF} protein localises to the nucleus and the mitochondria. Under normal conditions no co-localisation with PCNA could be detected, however following exposure to UV it was possible to co-immunoprecipitate p15{sup PAF} and PCNA from a number of cell lines, suggesting a UV-enhanced association of the two proteins. Overexpression of p15{sup PAF} in mammalian cells was also found to protect cells from UV-induced cell death. Based on similarities between the behaviour of p15{sup PAF} and the potential tumor suppressor product p33ING1b, we have further shown that these two proteins interact in the same complex in cell cultures. This suggests that p15{sup PAF} forms part of a larger protein complex potentially involved in the regulation of DNA repair, apoptosis and cell cycle progression.

• OSTI ID: 20775327
• Journal Information: Experimental Cell Research, Vol. 312, Issue 1; Other Information: DOI: 10.1016/j.yexcr.2005.09.020; PII: S0014-4827(05)00453-2; Copyright (c) 2005 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved; Country of input: International Atomic Energy Agency (IAEA); ISSN 0014-4827
• Country of Publication: United States
• Language: English

Related Subjects

60 APPLIED LIFE SCIENCES
ANTIGENS
APOPTOSIS
CELL CULTURES
CELL CYCLE
CELL PROLIFERATION
DNA REPAIR
DNA REPLICATION
IRRADIATION
MITOCHONDRIA
NEOPLASMS
PROTEINS
TUMOR CELLS
YEASTS