Fast neutrons-induced apoptosis is Fas-independent in lymphoblastoid cells
- Laboratoire de Cancerologie Experimentale et de Radiobiologie EA 3430, Universite Louis Pasteur, Institut de Recherche contre les Cancers de l'Appareil Digestif, Strasbourg (France)
- Laboratoire de Radiobiologie et de Radioprotection, Faculte de Medecine, Universite Catholique de Louvain, Brussels (Belgium)
We have previously shown that ionizing radiation-induced apoptosis in human lymphoblastoid cells differs according to their p53 status, and that caspase 8-mediated cleavage of BID is involved in the p53-dependent pathway. In the present study, we investigated the role of Fas signaling in caspase 8 activation induced by fast neutrons irradiation in these cells. Fas and FasL expression was assessed by flow cytometry and by immunoblot. We also measured Fas aggregation after irradiation by fluorescence microscopy. We found a decrease of Fas expression after irradiation, but no change in Fas ligand expression. We also showed that, in contrast to the stimulation of Fas by an agonistic antibody, Fas aggregation did not occur after irradiation. Altogether, our data strongly suggest that fast neutrons induced-apoptosis is Fas-independent, even in p53-dependent apoptosis.
- OSTI ID:
- 20710931
- Journal Information:
- Biochemical and Biophysical Research Communications, Vol. 334, Issue 2; Other Information: DOI: 10.1016/j.bbrc.2005.06.125; PII: S0006-291X(05)01351-3; Copyright (c) 2005 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved; Country of input: International Atomic Energy Agency (IAEA); ISSN 0006-291X
- Country of Publication:
- United States
- Language:
- English
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