Hardwood smoke alters murine splenic T cell responses to mitogens following a 6-month whole body inhalation exposure

Journal Article · Tue Feb 01 00:00:00 EST 2005 · Toxicology and Applied Pharmacology

DOI:https://doi.org/10.1016/j.taap.2004.06.024· OSTI ID:20634841

Burchiel, Scott W ^[1]; Lauer, Fredine T ^[1]; Dunaway, Sandy L ^[1]; Zawadzki, Jerome ^[2]; McDonald, Jacob D ^[3]; Reed, Matthew D ^[3]

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1. College of Pharmacy Toxicology Program, University of New Mexico, Albuquerque, NM 87131 (United States)
2. BD Biosciences Pharmingen Division, San Diego, CA 92121 (United States)
3. Lovelace Respiratory Research Institute, Albuquerque, NM 87109 (United States)

The purpose of these studies was to assess the effects of hardwood smoke (HWS) inhalation (30-1000 {mu}g/m{sup 3}) on the systemic immune responses of A/J mice evaluated after 6 months of daily exposures. Spleen cells obtained from mice were assessed for changes in cell number, cell surface marker expression [B, T, macrophage, and natural killer (NK) cells], and responses to B cell (LPS, endotoxin) and T cell (Con A) mitogens. Results showed that HWS smoke increased T cell proliferation in the 100 {mu}g/m{sup 3} exposure group and produced a concentration-dependent suppression of T cell proliferation at concentrations >300 {mu}g/m{sup 3}. There were no effects on B cell proliferation or in spleen cell surface marker expression. Analyses of the exposure atmospheres revealed the presence of significant levels of naphthalene and methylated napthalenes, fluorene, phenanthrene, and anthracene in the exposure chambers, as well as low concentrations of several metals (K, Ca, and Fe). Our results demonstrate that environmentally relevant concentrations of HWS may be immunosuppressive to the immune system of mice exposed during a 6-month period.

• OSTI ID: 20634841
• Journal Information: Toxicology and Applied Pharmacology, Vol. 202, Issue 3; Other Information: DOI: 10.1016/j.taap.2004.06.024; PII: S0041-008X(04)00335-7; Copyright (c) 2004 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved; Country of input: International Atomic Energy Agency (IAEA); ISSN 0041-008X
• Country of Publication: United States
• Language: English

Related Subjects

60 APPLIED LIFE SCIENCES
ANTHRACENE
CELL PROLIFERATION
FLUORENE
INHALATION
MACROPHAGES
MICE
MITOGENS
NAPHTHALENE
NATURAL KILLER CELLS
PHENANTHRENE
SMOKES
SPLEEN CELLS