¹²⁹ Xe NMR Relaxation-Based Macromolecular Sensing

Journal Article · Fri Jul 29 00:00:00 EDT 2016 · Journal of the American Chemical Society

DOI:https://doi.org/10.1021/jacs.6b02758· OSTI ID:1378351

Gomes, Muller D. ^[1]; Dao, Phuong ^[1]; Jeong, Keunhong ^[1]; Slack, Clancy C. ^[1]; Vassiliou, Christophoros C. ^[2]; Finbloom, Joel A. ^[2]; Francis, Matthew B. ^[1]; Wemmer, David E. ^[3]; Pines, Alexander ^[1]

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1. Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States). Materials Sciences Division; Univ. of California, Berkeley, CA (United States). Dept. of Chemistry
2. Univ. of California, Berkeley, CA (United States). Dept. of Chemistry
3. Univ. of California, Berkeley, CA (United States). Dept. of Chemistry; Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States). Physical Biosciences Division

A ¹²⁹Xe NMR relaxation-based sensing approach is reported on that exploits changes in the bulk xenon relaxation rate induced by slowed tumbling of a cryptophane-based sensor upon target binding. The amplification afforded by detection of the bulk dissolved xenon allows sensitive detection of targets. The sensor comprises a xenon-binding cryptophane cage, a target interaction element, and a metal chelating agent. Xenon associated with the target-bound cryptophane cage is rapidly relaxed and then detected after exchange with the bulk. Here we show that large macromolecular targets increase the rotational correlation time of xenon, increasing its relaxation rate. Upon binding of a biotin-containing sensor to avidin at 1.5 μM concentration, the free xenon T₂ is reduced by a factor of 4.

• Research Organization: Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
• Sponsoring Organization: USDOE Office of Science (SC), Basic Energy Sciences (BES)
• DOE Contract Number: AC02-05CH11231; DGE-1106400
• OSTI ID: 1378351
• Journal Information: Journal of the American Chemical Society, Vol. 138, Issue 31; ISSN 0002-7863
• Publisher: American Chemical Society (ACS)
• Country of Publication: United States
• Language: English

Related Subjects

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY