Profiling Changes in Histone Post-translational Modifications by Top-Down Mass Spectrometry

Book · Fri Nov 11 00:00:00 EST 2016

DOI:https://doi.org/10.1007/978-1-4939-6518-2_12· OSTI ID:1355115

Zhou, Mowei; Wu, Si; Stenoien, David L.; Zhang, Zhaorui; Connolly, Lanelle; Freitag, Michael; Pasa-Tolic, Ljiljana

Top-down mass spectrometry is a valuable tool for charactering post-translational modifications on histones for understanding of gene control and expression. In this protocol, we describe a top-down workflow using liquid chromatography coupled to mass spectrometry for fast global profiling of changes in histone proteoforms between a wild-type and a mutant of a fungal species. The proteoforms exhibiting different abundances can be subjected to further targeted studies by other mass spectrometric or biochemical assays. This method can be generally adapted for preliminary screening for changes in histone modifications between samples such as wild-type vs. mutant, and control vs. disease.

• Research Organization: Pacific Northwest National Lab. (PNNL), Richland, WA (United States). Environmental Molecular Sciences Lab. (EMSL)
• Sponsoring Organization: USDOE
• DOE Contract Number: AC05-76RL01830
• OSTI ID: 1355115
• Report Number(s): PNNL-SA-113153; 47802; KP1704020
• Resource Relation: Related Information: Methods in Molecular Biology: Eukaryotic transcriptional and post-transcriptional gene expression regulation, 1507:153-168
• Country of Publication: United States
• Language: English

Related Subjects

Environmental Molecular Sciences Laboratory