Analysis of the tet repressor-operator interactions using the uracil-DNA glycosylase footprinting system

Conference · Sat Dec 31 00:00:00 EST 1994

OSTI ID:134862

Devchand, P R; McGhee, J D; Sande, J.H. Van De ^[1]

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1. Univ. of Calgary, Alberta (Canada)

The tet repressor regulated expression of the Tn-10-encoded tetracycline resistance determinant in a tetracycline-dependent manner. In the absence of tetracycline, the tet repressor binds as a dimer to the 19-base-pair palindromic tet operator sequence. Amino acid homologies and genetic studies with trans-dominant mutants suggest that sequence-specific recognition of the tet operator involves the extensively studied helix-turn-helix motif. We have used the uracil-DNA glycosylase (UDG) footprinting systems to identify thymine contacts in the tet operator that are essential for the formation of tet repressor-operator complexes.

• Research Organization: New York Academy of Sciences, New York, NY (United States)
• OSTI ID: 134862
• Report Number(s): CONF-9307221-; TRN: 95:007741-0029
• Resource Relation: Conference: DNA damage: effects on DNA structure and protein recognition, Burlington, VT (United States), 31 Jul - 4 Aug 1993; Other Information: PBD: 1994; Related Information: Is Part Of DNA damage: Effects on DNA structure and protein recognition; Wallace, S.S.; Van Houten, B.; Kow, Yoke Wah [eds.]; PB: 395 p.
• Country of Publication: United States
• Language: English

Related Subjects

55 BIOLOGY AND MEDICINE
BASIC STUDIES
DNA
METHYLATION
DNA SEQUENCING
THYMINE
DETECTION
TETRACYCLINES
GENETIC EFFECTS
PROTEINS
STRUCTURE-ACTIVITY RELATIONSHIPS
BIOLOGICAL MODELS
URACILS
MUTANTS
DIMERS
AMINO ACIDS
DNA HELICASES