Compositions and methods for making selenocysteine containing polypeptides
Patent
·
OSTI ID:1328702
Non-naturally occurring tRNA.sup.Sec and methods of using them for recombinant expression of proteins engineered to include one or more selenocysteine residues are disclosed. The non-naturally occurring tRNA.sup.Sec can be used for recombinant manufacture of selenocysteine containing polypeptides encoded by mRNA without the requirement of an SECIS element. In some embodiments, selenocysteine containing polypeptides are manufactured by co-expressing a non-naturally occurring tRNA.sup.Sec a recombinant expression system, such as E. coli, with SerRS, EF-Tu, SelA, or PSTK and SepSecS, and an mRNA with at least one codon that recognizes the anticodon of the non-naturally occurring tRNA.sup.Sec.
- Research Organization:
- Yale Univ., New Haven, CT (United States)
- Sponsoring Organization:
- USDOE
- DOE Contract Number:
- FG02-98ER20311
- Assignee:
- Yale University (New Haven, CT)
- Patent Number(s):
- 9,464,288
- Application Number:
- 14/131,382
- OSTI ID:
- 1328702
- Resource Relation:
- Patent File Date: 2012 Jul 11
- Country of Publication:
- United States
- Language:
- English
Similar Records
Compositions and methods for making selenocysteine containing polypeptides
Compositions and methods for making selenocysteine containing polypeptides
Compositions and methods for making selenocysteine containing polypeptides
Patent
·
Tue Jul 17 00:00:00 EDT 2018
·
OSTI ID:1328702
Compositions and methods for making selenocysteine containing polypeptides
Patent
·
Tue Mar 26 00:00:00 EDT 2019
·
OSTI ID:1328702
+1 more
Compositions and methods for making selenocysteine containing polypeptides
Patent
·
Tue Dec 29 00:00:00 EST 2020
·
OSTI ID:1328702
+1 more