Lignases and aldo-keto reductases for conversion of lignin-containing materials to fermentable products
Termites have specialized digestive systems that overcome the lignin barrier in wood to release fermentable simple sugars. Using the termite Reticulitermes flavipes and its gut symbionts, high-throughput titanium pyrosequencing and proteomics approaches experimentally compared the effects of lignin-containing diets on host-symbiont digestome composition. Proteomic investigations and functional digestive studies with recombinant lignocellulases conducted in parallel provided strong evidence of congruence at the transcription and translational levels and provide enzymatic strategies for overcoming recalcitrant lignin barriers in biofuel feedstocks. Briefly described, therefore, the disclosure provides a system for generating a fermentable product from a lignified plant material, the system comprising a cooperating series of at least two catalytically active polypeptides, where said catalytically active polypeptides are selected from the group consisting of: cellulase Cell-1, $$\beta$$-glu cellulase, an aldo-keto-reductase, a catalase, a laccase, and an endo-xylanase.
- Research Organization:
- Purdue Univ., West Lafayette, IN (United States)
- Sponsoring Organization:
- USDOE
- DOE Contract Number:
- FC03-97ER62402
- Assignee:
- Purdue Research Foundation (West Lafayette, IN)
- Patent Number(s):
- 9,441,256
- Application Number:
- 14/379,990
- OSTI ID:
- 1324695
- Resource Relation:
- Patent File Date: 2013 Feb 11
- Country of Publication:
- United States
- Language:
- English
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