Three-dimensional imaging of cholesterol and sphingolipids within a Madin-Darby canine kidney cell

Yeager, Ashley N.; Weber, Peter K.; Kraft, Mary L.

doi:10.1116/1.4939681

Title: Three-dimensional imaging of cholesterol and sphingolipids within a Madin-Darby canine kidney cell

Journal Article · Fri Jan 08 00:00:00 EST 2016 · Biointerphases

DOI:https://doi.org/10.1116/1.4939681· OSTI ID:1305902

Yeager, Ashley N. ^[1]; Weber, Peter K. ^[2]; Kraft, Mary L. ^[1]

Univ. of Illinois, Urbana, IL (United States). Dept. of Chemical and Biomolecular Engineering
Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States). Glenn T. Seaborg Institute

Metabolic stable isotope incorporation and secondary ion mass spectrometry(SIMS) depth profiling performed on a Cameca NanoSIMS 50 were used to image the ¹⁸O-cholesterol and ¹⁵N-sphingolipid distributions within a portion of a Madin-Darby canine kidney (MDCK) cell. Three-dimensional representations of the component-specific isotope distributions show clearly defined regions of ¹⁸O-cholesterol and ¹⁵N-sphingolipid enrichment that seem to be separate subcellular compartments. Furthermore, the low levels of nitrogen-containing secondary ions detected at the ¹⁸O-enriched regions suggest that these ¹⁸O-cholesterol-rich structures may be lipiddroplets, which have a core consisting of cholesterol esters and triacylglycerides.

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Research Organization:: Lawrence Livermore National Laboratory (LLNL), Livermore, CA (United States)

Sponsoring Organization:: USDOE

Grant/Contract Number:: AC52-07NA27344

OSTI ID:: 1305902

Alternate ID(s):: OSTI ID: 1421226

Report Number(s):: LLNL-JRNL-680575; BJIOBN

Journal Information:: Biointerphases, Vol. 11, Issue 2; ISSN 1934-8630

Publisher:: AIPCopyright Statement

Country of Publication:: United States

Language:: English

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Cited by: 21 works

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Title: Three-dimensional imaging of cholesterol and sphingolipids within a Madin-Darby canine kidney cell

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