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Title: Carbon dioxide "trapped" in a β-carbonic anhydrase

Journal Article · · Biochemistry
 [1];  [2];  [3];  [2];  [3]
  1. Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
  2. Cornell Univ., Ithaca, NY (United States)
  3. Univ. of Florida, Gainesville, FL (United States)
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Carbonic anhydrases (CAs) are enzymes that catalyze the hydration/ dehydration of CO2/HCO3- with rates approaching diffusion-controlled limits (kcat/KM ~ 108 M–1s–1). Here, this family of enzymes has evolved disparate protein folds that all perform the same reaction at near catalytic perfection. Presented here is a structural study of a beta-CA (psCA3) expressed in Pseudomonas aeruginosa, in complex with CO2, using pressurized cryocooled crystallography. The structure has been refined to 1.6 angstrom resolution with Rcryst and Rfree values of 17.3 and 19.9%, respectively, and is compared with the α-CA, human CA isoform II (hCA II), the only other CA to have CO2, captured in its active site. Despite the lack of structural similarity between psCA3 and hCA II, the CO2, binding orientation relative to the zinc-bound solvent is identical. In addition, a second CO2, binding site was located at the dimer interface of psCA3. Interestingly, all β-CAs function as dirners or higher-order oligomeric states, and the CO2, bound at the interface may contribute to the allosteric nature of this family of enzymes or may be a convenient alternative binding site as this pocket has been previously shown to be a promiscuous site for a variety of ligands, including bicarbonate, sulfate, and phosphate ions.

Research Organization:
Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States)
Sponsoring Organization:
USDOE
Grant/Contract Number:
AC05-00OR22725
OSTI ID:
1287017
Journal Information:
Biochemistry, Vol. 54, Issue 43; ISSN 0006-2960
Publisher:
American Chemical Society (ACS)Copyright Statement
Country of Publication:
United States
Language:
English
Citation Metrics:
Cited by: 17 works
Citation information provided by
Web of Science

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Cited By (4)

Carbon dioxide to bio‐fuels by mixed and pure microbial cultures isolated from activated sludge: relative evaluation of CO 2 fixation, biodiesel production, and thermodynamic analysis journal August 2019
Kinetic study of catalytic CO 2 hydration by metal-substituted biomimetic carbonic anhydrase model complexes journal August 2019
Structure and function of carbonic anhydrases journal January 1977
Structure and Function of Carbonic Anhydrases book January 1981

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Related Subjects

59 BASIC BIOLOGICAL SCIENCES
β-carbonic anhydrase
Pseudomonas aeruginosa
CO2 binding