Screening and modular design for metabolic pathway optimization

Boock, Jason T.; Gupta, Apoorv; Prather, Kristala LJ

doi:10.1016/j.copbio.2015.08.013

Title: Screening and modular design for metabolic pathway optimization

Journal Article · Thu Oct 01 00:00:00 EDT 2015 · Current Opinion in Biotechnology

DOI:https://doi.org/10.1016/j.copbio.2015.08.013· OSTI ID:1548304

Boock, Jason T. ^[1]; Gupta, Apoorv ^[1];

^[1]

Massachusetts Inst. of Technology (MIT), Cambridge, MA (United States)

Biological conversion of substrate sugars to a variety of products is an increasingly popular option for chemical transformation due to its high specificity and because of significant interest in the use of renewable feedstocks. Yet, pathway optimization through metabolic engineering is often needed to make such molecules economically at a relevant scale. Employing effective methods to search and narrow the immense pathway parameter space is essential to meet performance metrics such as high titer, yield and productivity with efficiency. This review focuses on two practices that increase the likelihood of finding a more advantageous pathway solution: implementing a screen to identify high producers and utilizing modular pathway design to streamline engineering efforts. While screens seek to couple product titer with a high-throughput measurement output, modular design aims to rationally construct pathways to allow parallel optimization of various units. Both of these methodologies have proven widely successful in metabolic engineering, with combinations of them resulting in synergistic enhancements to pathway optimization. This review will specifically illustrate their utility for microbially derived acid and alcohol products, which are of interest as fuels and value added products.

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Research Organization:: Massachusetts Inst. of Technology (MIT), Cambridge, MA (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Biological and Environmental Research (BER); National Science Foundation (NSF); Institute for Collaborative Biotechnologies

Grant/Contract Number:: SC0012555; EEC-0540879; W911NF-09-0001

OSTI ID:: 1548304

Alternate ID(s):: OSTI ID: 1250328

Journal Information:: Current Opinion in Biotechnology, Vol. 36, Issue C; ISSN 0958-1669

Publisher:: ElsevierCopyright Statement

Country of Publication:: United States

Language:: English

Citation Metrics:

Cited by: 19 works

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59 BASIC BIOLOGICAL SCIENCES
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microbial alcohol production
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Title: Screening and modular design for metabolic pathway optimization

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