Metalloprotein structures at ambient conditions and in real-time: biological crystallography and spectroscopy using X-ray free electron lasers

Kern, Jan; Yachandra, Vittal K.; Yano, Junko

doi:10.1016/j.sbi.2015.07.014

Title: Metalloprotein structures at ambient conditions and in real-time: biological crystallography and spectroscopy using X-ray free electron lasers

Journal Article · Wed Sep 02 00:00:00 EDT 2015 · Current Opinion in Structural Biology

DOI:https://doi.org/10.1016/j.sbi.2015.07.014· OSTI ID:1378579

Kern, Jan ^[1]; Yachandra, Vittal K. ^[2]; Yano, Junko ^[2]

Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States). Physical Biosciences Division; SLAC National Accelerator Lab., Menlo Park, CA (United States). Linac Coherent Light Source
Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States). Physical Biosciences Division

We have studied the structure of enzymes and the chemistry at the catalytic sites, intensively and have acquired an understanding of the atomic-scale chemistry which requires a new approach beyond steady state X-ray crystallography and X-ray spectroscopy at cryogenic temperatures. Following the dynamic changes in the geometric and electronic structure of metallo-enzymes at ambient conditions, while overcoming the severe X-ray-induced changes to the redox active catalytic center, is key for deriving reaction mechanisms. Such studies become possible by the intense and ultra-short femtosecond (fs) X-ray pulses from an X-ray free electron laser (XFEL) by acquiring a signal before the sample is destroyed. Our review describes the recent and pioneering uses of XFELs to study the protein structure and dynamics of metallo-enzymes using crystallography and scattering, as well as the chemical structure and dynamics of the catalytic complexes (charge, spin, and covalency) using spectroscopy during the reaction to understand the electron-transfer processes and elucidate the mechanism.

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Research Organization:: Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Basic Energy Sciences (BES)

Grant/Contract Number:: AC02-05CH11231

OSTI ID:: 1378579

Alternate ID(s):: OSTI ID: 1250107

Journal Information:: Current Opinion in Structural Biology, Vol. 34, Issue C; ISSN 0959-440X

Publisher:: ElsevierCopyright Statement

Country of Publication:: United States

Language:: English

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Cited by: 33 works

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