Clostridium thermocellum DSM 1313 transcriptional responses to redox perturbation

Sander, Kyle; Wilson, Charlotte M.; Rodriguez, Jr., Miguel; Klingeman, Dawn M.; Rydzak, Thomas; Davison, Brian H.; Brown, Steven D.

doi:10.1186/s13068-015-0394-9

Title: Clostridium thermocellum DSM 1313 transcriptional responses to redox perturbation

Journal Article · Sat Dec 12 00:00:00 EST 2015 · Biotechnology for Biofuels

DOI:https://doi.org/10.1186/s13068-015-0394-9· OSTI ID:1618631

Sander, Kyle; Wilson, Charlotte M.; Rodriguez, Jr., Miguel; Klingeman, Dawn M.; Rydzak, Thomas; Davison, Brian H.; Brown, Steven D.

Clostridium thermocellum is a promising consolidated bioprocessing candidate organism capable of directly converting lignocellulosic biomass to ethanol. Current ethanol yields, productivities, and growth inhibitions are industrial deployment impediments for commodity fuel production by this bacterium. Redox imbalance under certain conditions and in engineered strains may contribute to incomplete substrate utilization and may direct fermentation products to undesirable overflow metabolites. As a result, towards a better understanding of redox metabolism in C. thermocellum, we established continuous growth conditions and analyzed global gene expression during addition of two stress chemicals (methyl viologen and hydrogen peroxide) which changed the fermentation redox potential.

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Research Organization:: Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States)

Sponsoring Organization:: USDOE Office of Science (SC)

Grant/Contract Number:: AC05-00OR22725

OSTI ID:: 1618631

Alternate ID(s):: OSTI ID: 1238009

Journal Information:: Biotechnology for Biofuels, Journal Name: Biotechnology for Biofuels Vol. 8 Journal Issue: 1; ISSN 1754-6834

Publisher:: Springer Science + Business MediaCopyright Statement

Country of Publication:: Netherlands

Language:: English

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Cited by: 18 works

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Related Subjects

59 BASIC BIOLOGICAL SCIENCES
Clostridium thermocellum DSM 1313
microarray
transcriptomics
methyl viologen
chemostat
redox
sulfate
GS-GOGAT
hydrogenase

Clostridium thermocellum DSM 1313 transcriptional responses to redox perturbation [Supplemental Data] Sander, Kyle; Wilson, Charlotte; Rodriguez, Miguel https://doi.org/10.6084/m9.figshare.c.3613466 The current record is supplemented by this dataset	dataset	December 2015
MOESM3 of Clostridium thermocellum DSM 1313 transcriptional responses to redox perturbation Sander, Kyle; Wilson, Charlotte; Rodriguez, Miguel https://doi.org/10.6084/m9.figshare.c.3613466_d1.v1 The current record is supplemented by this dataset	dataset	December 2015
MOESM1 of Clostridium thermocellum DSM 1313 transcriptional responses to redox perturbation Sander, Kyle; Wilson, Charlotte; Rodriguez, Miguel https://doi.org/10.6084/m9.figshare.c.3613466_d2.v1 The current record is supplemented by this dataset	dataset	December 2015
MOESM2 of Clostridium thermocellum DSM 1313 transcriptional responses to redox perturbation Sander, Kyle; Wilson, Charlotte; Rodriguez, Miguel https://doi.org/10.6084/m9.figshare.c.3613466_d3 The current record is supplemented by this dataset	dataset	January 2015
MOESM2 of Clostridium thermocellum DSM 1313 transcriptional responses to redox perturbation Sander, Kyle; Wilson, Charlotte; Rodriguez, Miguel https://doi.org/10.6084/m9.figshare.c.3613466_d3.v1 The current record is supplemented by this dataset	dataset	December 2015
MOESM4 of Clostridium thermocellum DSM 1313 transcriptional responses to redox perturbation Sander, Kyle; Wilson, Charlotte; Rodriguez, Miguel https://doi.org/10.6084/M9.FIGSHARE.C.3613466_D4 The current record is supplemented by this dataset	dataset	January 2015
MOESM4 of Clostridium thermocellum DSM 1313 transcriptional responses to redox perturbation Sander, Kyle; Wilson, Charlotte; Rodriguez, Miguel https://doi.org/10.6084/m9.figshare.c.3613466_d4.v1 The current record is supplemented by this dataset	dataset	December 2015
Clostridium thermocellum DSM 1313 transcriptional responses to redox perturbation Sander, Kyle; Wilson, Charlotte; Rodriguez, Miguel https://doi.org/10.6084/m9.figshare.c.3613466.v1 The current record is supplemented by this dataset	dataset	December 2015
MOESM3 of Clostridium thermocellum DSM 1313 transcriptional responses to redox perturbation Sander, Kyle; Wilson, Charlotte; Rodriguez, Miguel https://doi.org/10.6084/m9.figshare.c.3613466_d1 The current record is supplemented by this text	text	January 2015