Simultaneous cryo X-ray ptychographic and fluorescence microscopy of green algae

Deng, Junjing; Vine, David J.; Chen, Si; Nashed, Youssef S. G.; Jin, Qiaoling; Phillips, Nicholas W.; Peterka, Tom; Ross, Rob; Vogt, Stefan; Jacobsen, Chris J.

doi:10.1073/pnas.1413003112

Title: Simultaneous cryo X-ray ptychographic and fluorescence microscopy of green algae

Journal Article · Mon Feb 09 00:00:00 EST 2015 · Proceedings of the National Academy of Sciences of the United States of America

DOI:https://doi.org/10.1073/pnas.1413003112· OSTI ID:1235500

Deng, Junjing ^[1]; Vine, David J. ^[2]; Chen, Si ^[2]; Nashed, Youssef S. G. ^[3]; Jin, Qiaoling ^[4]; Phillips, Nicholas W. ^[5]; Peterka, Tom ^[3]; Ross, Rob ^[3]; Vogt, Stefan ^[2]; Jacobsen, Chris J. ^[6]

Applied Physics, Northwestern University, Evanston, IL 60208,
X-ray Science Division, Advanced Photon Source, Argonne National Laboratory, Argonne, IL 60439,
Mathematics and Computing Science Division, Argonne National Laboratory, Argonne, IL 60439,
Department of Physics and Astronomy, Northwestern University, Evanston, IL 60208,
Australian Research Council, Centre of Excellence for Advanced Molecular Imaging, La Trobe University, Melbourne, VIC 3086, Australia,, Australian Research Council, Centre of Excellence for Coherent X-ray Science, La Trobe University, Melbourne, VIC 3086, Australia,, Commonwealth Scientific and Industrial Research Organization Manufacturing Flagship, Parkville, VIC 3052, Australia, and
Applied Physics, Northwestern University, Evanston, IL 60208,, Department of Physics and Astronomy, Northwestern University, Evanston, IL 60208,, Chemistry of Life Processes Institute, Northwestern University, Evanston, IL 60208

Trace metals play important roles in normal and in disease-causing biological functions. X-ray fluorescence microscopy reveals trace elements with no dependence on binding affinities (unlike with visible light fluorophores) and with improved sensitivity relative to electron probes. However, X-ray fluorescence is not very sensitive for showing the light elements that comprise the majority of cellular material. Here we show that X-ray ptychography can be combined with fluorescence to image both cellular structure and trace element distribution in frozen-hydrated cells at cryogenic temperatures, with high structural and chemical fidelity. Ptychographic reconstruction algorithms deliver phase and absorption contrast images at a resolution beyond that of the illuminating lens or beam size. Using 5.2-keV X-rays, we have obtained sub-30-nm resolution structural images and ~90-nm-resolution fluorescence images of several elements in frozen-hydrated green algae. Finally, this combined approach offers a way to study the role of trace elements in their structural context.

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Research Organization:: Argonne National Laboratory (ANL), Argonne, IL (United States)

Sponsoring Organization:: National Institutes of Health (NIH); USDOE Office of Science (SC), Basic Energy Sciences (BES)

Grant/Contract Number:: AC02-06CH11357; 1R01GM104530; 1S10RR029272-01

OSTI ID:: 1235500

Alternate ID(s):: OSTI ID: 1215636; OSTI ID: 1346725

Journal Information:: Proceedings of the National Academy of Sciences of the United States of America, Journal Name: Proceedings of the National Academy of Sciences of the United States of America Vol. 112 Journal Issue: 8; ISSN 0027-8424

Publisher:: Proceedings of the National Academy of SciencesCopyright Statement

Country of Publication:: United States

Language:: English

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Cited by: 108 works

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