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Title: GenomePeek—an online tool for prokaryotic genome and metagenome analysis

Journal Article · · PeerJ
DOI:https://doi.org/10.7717/peerj.1025· OSTI ID:1221899
 [1];  [2]
  1. San Diego State University, San Diego, CA (United States). Department of Computer Science; San Diego State University, San Diego, CA (United States). Department of Biology
  2. San Diego State University, San Diego, CA (United States). Department of Computer Science; San Diego State University, San Diego, CA (United States). Department of Biology; San Diego State University, San Diego, CA (United States). Computational Sciences Research Center; Argonne National Lab. (ANL), Argonne, IL (United States)

As increases in prokaryotic sequencing take place, a method to quickly and accurately analyze this data is needed. Previous tools are mainly designed for metagenomic analysis and have limitations; such as long runtimes and significant false positive error rates. The online tool GenomePeek (edwards.sdsu.edu/GenomePeek) was developed to analyze both single genome and metagenome sequencing files, quickly and with low error rates. GenomePeek uses a sequence assembly approach where reads to a set of conserved genes are extracted, assembled and then aligned against the highly specific reference database. GenomePeek was found to be faster than traditional approaches while still keeping error rates low, as well as offering unique data visualization options.

Research Organization:
Argonne National Laboratory (ANL), Argonne, IL (United States)
Sponsoring Organization:
USDOE
Contributing Organization:
National Science Foundation (NSF), Washington, DC (United States)
Grant/Contract Number:
AC02-06CH11357
OSTI ID:
1221899
Journal Information:
PeerJ, Vol. 3; ISSN 2167-8359
Publisher:
PeerJ Inc.Copyright Statement
Country of Publication:
United States
Language:
English
Citation Metrics:
Cited by: 10 works
Citation information provided by
Web of Science

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Correcting for 16S rRNA gene copy numbers in microbiome surveys remains an unsolved problem journal February 2018
Intermediate-Salinity Systems at High Altitudes in the Peruvian Andes Unveil a High Diversity and Abundance of Bacteria and Viruses journal November 2019
Correcting for 16S rRNA gene copy numbers in microbiome surveys remains an unsolved problem text January 2018