Forest floor community metatranscriptomes identify fungal and bacterial responses to N deposition in two maple forests

Hesse, Cedar N.; Mueller, Rebecca C.; Vuyisich, Momchilo; Gallegos-Graves, La Verne; Gleasner, Cheryl D.; Zak, Donald R.; Kuske, Cheryl R.

doi:10.3389/fmicb.2015.00337

Title: Forest floor community metatranscriptomes identify fungal and bacterial responses to N deposition in two maple forests

Journal Article · Thu Apr 23 00:00:00 EDT 2015 · Frontiers in Microbiology

DOI:https://doi.org/10.3389/fmicb.2015.00337· OSTI ID:1200903

Hesse, Cedar N. ^[1]; Mueller, Rebecca C. ^[1]; Vuyisich, Momchilo ^[1]; Gallegos-Graves, La Verne ^[1]; Gleasner, Cheryl D. ^[1]; Zak, Donald R. ^[2]; Kuske, Cheryl R. ^[1]

Los Alamos National Lab. (LANL), Los Alamos, NM (United States)
Univ. of Michigan, Ann Arbor, MI (United States)

Anthropogenic N deposition alters patterns of C and N cycling in temperate forests, where forest floor litter decomposition is a key process mediated by a diverse community of bacteria and fungi. To track forest floor decomposer activity we generated metatranscriptomes that simultaneously surveyed the actively expressed bacterial and eukaryote genes in the forest floor, to compare the impact of N deposition on the decomposers in two natural maple forests in Michigan, USA, where replicate field plots had been amended with N for 16 years. Site and N amendment responses were compared using about 74,000 carbohydrate active enzyme transcript sequences (CAZymes) in each metatranscriptome. Parallel ribosomal RNA (rRNA) surveys of bacterial and fungal biomass and taxonomic composition showed no significant differences in either biomass or OTU richness between the two sites or in response to N. Site and N amendment were not significant variables defining bacterial taxonomic composition, but they were significant for fungal community composition, explaining 17 and 14% of the variability, respectively. The relative abundance of expressed bacterial and fungal CAZymes changed significantly with N amendment in one of the forests, and N-response trends were also identified in the second forest. Although the two ambient forests were similar in community biomass, taxonomic structure and active CAZyme profile, the shifts in active CAZyme profiles in response to N-amendment differed between the sites. One site responded with an over-expression of bacterial CAZymes, and the other site responded with an over-expression of both fungal and different bacterial CAZymes. Both sites showed reduced representation of fungal lignocellulose degrading enzymes in N-amendment plots. The metatranscriptome approach provided a holistic assessment of eukaryote and bacterial gene expression and is applicable to other systems where eukaryotes and bacteria interact.

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Research Organization:: Los Alamos National Laboratory (LANL), Los Alamos, NM (United States)

Sponsoring Organization:: USDOE

Grant/Contract Number:: AC52-06NA25396

OSTI ID:: 1200903

Journal Information:: Frontiers in Microbiology, Vol. 6; ISSN 1664-302X

Publisher:: Frontiers Research FoundationCopyright Statement

Country of Publication:: United States

Language:: English

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Cited by: 63 works

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References (56)

Are Basidiomycete Laccase Gene Abundance and Composition Related to Reduced Lignolytic Activity Under Elevated Atmospheric NO3 − Deposition in a Northern Hardwood Forest? Hassett, John E.; Zak, Donald R.; Blackwood, Christopher B. Microbial Ecology, Vol. 57, Issue 4 https://doi.org/10.1007/s00248-008-9440-5	journal	September 2008
Nitrogen Cycles: Past, Present, and Future Galloway, J. N.; Dentener, F. J.; Capone, D. G. Biogeochemistry, Vol. 70, Issue 2 https://doi.org/10.1007/s10533-004-0370-0	journal	September 2004
Ecology of ligninolytic fungi associated with leaf litter decomposition Osono, Takashi Ecological Research, Vol. 22, Issue 6 https://doi.org/10.1007/s11284-007-0390-z	journal	June 2007
Chronic nitrogen deposition reduces the abundance of dominant forest understory and groundcover species Talhelm, Alan F.; Burton, Andrew J.; Pregitzer, Kurt S. Forest Ecology and Management, Vol. 293 https://doi.org/10.1016/j.foreco.2012.12.020	journal	April 2013
Tree species influence on microbial communities in litter and soil: Current knowledge and research needs Prescott, Cindy E.; Grayston, Sue J. Forest Ecology and Management, Vol. 309 https://doi.org/10.1016/j.foreco.2013.02.034	journal	December 2013
Microbial responses to a changing environment: implications for the future functioning of terrestrial ecosystems Zak, Donald R.; Pregitzer, Kurt S.; Burton, Andrew J. Fungal Ecology, Vol. 4, Issue 6 https://doi.org/10.1016/j.funeco.2011.04.001	journal	December 2011
Prospects and challenges for fungal metatranscriptomics of complex communities Kuske, Cheryl R.; Hesse, Cedar N.; Challacombe, Jean F. Fungal Ecology, Vol. 14 https://doi.org/10.1016/j.funeco.2014.12.005	journal	April 2015
Reverse transcription-PCR methods significantly impact richness and composition measures of expressed fungal cellobiohydrolase I genes in soil and litter Weber, Carolyn F.; Kuske, Cheryl R. Journal of Microbiological Methods, Vol. 86, Issue 3 https://doi.org/10.1016/j.mimet.2011.06.011	journal	September 2011
Soil eukaryotic functional diversity, a metatranscriptomic approach Bailly, Julie; Fraissinet-Tachet, Laurence; Verner, Marie-Christine The ISME Journal, Vol. 1, Issue 7 https://doi.org/10.1038/ismej.2007.68	journal	September 2007
Who is who in litter decomposition? Metaproteomics reveals major microbial players and their biogeochemical functions Schneider, Thomas; Keiblinger, Katharina M.; Schmid, Emanuel The ISME Journal, Vol. 6, Issue 9 https://doi.org/10.1038/ismej.2012.11	journal	March 2012
Organic carbon transformations in high-Arctic peat soils: key functions and microorganisms Tveit, Alexander; Schwacke, Rainer; Svenning, Mette M. The ISME Journal, Vol. 7, Issue 2 https://doi.org/10.1038/ismej.2012.99	journal	September 2012
Comparative metatranscriptomics reveals kingdom level changes in the rhizosphere microbiome of plants Turner, Thomas R.; Ramakrishnan, Karunakaran; Walshaw, John The ISME Journal, Vol. 7, Issue 12 https://doi.org/10.1038/ismej.2013.119	journal	July 2013
Global nitrogen deposition and carbon sinks Reay, Dave S.; Dentener, Frank; Smith, Pete Nature Geoscience, Vol. 1, Issue 7 https://doi.org/10.1038/ngeo230	journal	June 2008
Reduction of forest soil respiration in response to nitrogen deposition Janssens, I. A.; Dieleman, W.; Luyssaert, S. Nature Geoscience, Vol. 3, Issue 5 https://doi.org/10.1038/ngeo844	journal	April 2010
Accurate determination of microbial diversity from 454 pyrosequencing data Quince, Christopher; Lanzén, Anders; Curtis, Thomas P. Nature Methods, Vol. 6, Issue 9 https://doi.org/10.1038/nmeth.1361	journal	August 2009
Red versus green leaves: transcriptomic comparison of foliar senescence between two Prunus cerasifera genotypes Vangelisti, Alberto; Guidi, Lucia; Cavallini, Andrea Scientific Reports, Vol. 10, Issue 1 https://doi.org/10.1038/s41598-020-58878-8	journal	February 2020
Infant gut microbiota characteristics generally do not modify effects of lipid-based nutrient supplementation on growth or inflammation: secondary analysis of a randomized controlled trial in Malawi Hughes, Riley L.; Arnold, Charles D.; Young, Rebecca R. Scientific Reports, Vol. 10, Issue 1 https://doi.org/10.1038/s41598-020-71922-x	journal	September 2020
UniRef: comprehensive and non-redundant UniProt reference clusters Suzek, B. E.; Huang, H.; McGarvey, P. Bioinformatics, Vol. 23, Issue 10 https://doi.org/10.1093/bioinformatics/btm098	journal	March 2007
Search and clustering orders of magnitude faster than BLAST Edgar, Robert C. Bioinformatics, Vol. 26, Issue 19, p. 2460-2461 https://doi.org/10.1093/bioinformatics/btq461	journal	August 2010
SortMeRNA: fast and accurate filtering of ribosomal RNAs in metatranscriptomic data Kopylova, Evguenia; Noé, Laurent; Touzet, Hélène Bioinformatics, Vol. 28, Issue 24 https://doi.org/10.1093/bioinformatics/bts611	journal	October 2012
Rfam: an RNA family database Griffiths-Jones, S. Nucleic Acids Research, Vol. 31, Issue 1 https://doi.org/10.1093/nar/gkg006	journal	January 2003
SILVA: a comprehensive online resource for quality checked and aligned ribosomal RNA sequence data compatible with ARB Pruesse, E.; Quast, C.; Knittel, K. Nucleic Acids Research, Vol. 35, Issue 21 https://doi.org/10.1093/nar/gkm864	journal	November 2007
The Carbohydrate-Active EnZymes database (CAZy): an expert resource for Glycogenomics Cantarel, B. L.; Coutinho, P. M.; Rancurel, C. Nucleic Acids Research, Vol. 37, Issue Database https://doi.org/10.1093/nar/gkn663	journal	January 2009
Rfam: updates to the RNA families database Gardner, P. P.; Daub, J.; Tate, J. G. Nucleic Acids Research, Vol. 37, Issue Database https://doi.org/10.1093/nar/gkn766	journal	January 2009
The Ribosomal Database Project: improved alignments and new tools for rRNA analysis Cole, J. R.; Wang, Q.; Cardenas, E. Nucleic Acids Research, Vol. 37, Issue Database https://doi.org/10.1093/nar/gkn879	journal	January 2009
Comparison of two next-generation sequencing technologies for resolving highly complex microbiota composition using tandem variable 16S rRNA gene regions Claesson, Marcus J.; Wang, Qiong; O'Sullivan, Orla Nucleic Acids Research, Vol. 38, Issue 22 https://doi.org/10.1093/nar/gkq873	journal	September 2010
The SILVA ribosomal RNA gene database project: improved data processing and web-based tools Quast, Christian; Pruesse, Elmar; Yilmaz, Pelin Nucleic Acids Research, Vol. 41, Issue D1 https://doi.org/10.1093/nar/gks1219	journal	November 2012
dbCAN: a web resource for automated carbohydrate-active enzyme annotation Yin, Yanbin; Mao, Xizeng; Yang, Jincai Nucleic Acids Research, Vol. 40, Issue W1 https://doi.org/10.1093/nar/gks479	journal	May 2012
Data, information, knowledge and principle: back to metabolism in KEGG Kanehisa, Minoru; Goto, Susumu; Sato, Yoko Nucleic Acids Research, Vol. 42, Issue D1 https://doi.org/10.1093/nar/gkt1076	journal	November 2013
MEGAN analysis of metagenomic data Huson, D. H.; Auch, A. F.; Qi, J. Genome Research, Vol. 17, Issue 3 https://doi.org/10.1101/gr.5969107	journal	February 2007
Dryland soil microbial communities display spatial biogeographic patterns associated with soil depth and soil parent material Steven, Blaire; Gallegos-Graves, La Verne; Belnap, Jayne FEMS Microbiology Ecology, Vol. 86, Issue 1 https://doi.org/10.1111/1574-6941.12143	journal	May 2013
Microbial models with data-driven parameters predict stronger soil carbon responses to climate change Hararuk, Oleksandra; Smith, Matthew J.; Luo, Yiqi Global Change Biology, Vol. 21, Issue 6 https://doi.org/10.1111/gcb.12827	journal	March 2015
Simulated chronic NO ₃ ⁻ deposition reduces soil respiration in northern hardwood forests Burton, Andrew J.; Pregitzer, Kurt S.; Crawford, Jeffrey N. Global Change Biology, Vol. 10, Issue 7 https://doi.org/10.1111/j.1365-2486.2004.00737.x	journal	July 2004
Simulated chronic nitrogen deposition increases carbon storage in Northern Temperate forests Pregitzer, Kurt S.; Burton, Andrew J.; Zak, Donald R. Global Change Biology, Vol. 0, Issue 0 https://doi.org/10.1111/j.1365-2486.2007.01465.x	journal	November 2007
The unseen majority: soil microbes as drivers of plant diversity and productivity in terrestrial ecosystems van der Heijden, Marcel G. A.; Bardgett, Richard D.; van Straalen, Nico M. Ecology Letters, Vol. 11, Issue 3 https://doi.org/10.1111/j.1461-0248.2007.01139.x	journal	March 2008
A global perspective on belowground carbon dynamics under nitrogen enrichment: Belowground C dynamics under N enrichment Liu, Lingli; Greaver, Tara L. Ecology Letters, Vol. 13, Issue 7 https://doi.org/10.1111/j.1461-0248.2010.01482.x	journal	May 2010
Soil respiration, root biomass, and root turnover following long-term exposure of northern forests to elevated atmospheric CO ₂ and tropospheric O ₃ Pregitzer, Kurt S.; Burton, Andrew J.; King, John S. New Phytologist, Vol. 180, Issue 1 https://doi.org/10.1111/j.1469-8137.2008.02564.x	journal	October 2008
Atmospheric N Deposition Increases Bacterial Laccase-Like Multicopper Oxidases: Implications for Organic Matter Decay Freedman, Zachary; Zak, Donald R. Applied and Environmental Microbiology, Vol. 80, Issue 14 https://doi.org/10.1128/aem.01224-14	journal	May 2014
Introducing mothur: Open-Source, Platform-Independent, Community-Supported Software for Describing and Comparing Microbial Communities Schloss, P. D.; Westcott, S. L.; Ryabin, T. Applied and Environmental Microbiology, Vol. 75, Issue 23, p. 7537-7541 https://doi.org/10.1128/aem.01541-09	journal	October 2009
Microbial Mechanisms Mediating Increased Soil C Storage under Elevated Atmospheric N Deposition Eisenlord, Sarah D.; Freedman, Zachary; Zak, Donald R. Applied and Environmental Microbiology, Vol. 79, Issue 4 https://doi.org/10.1128/aem.03156-12	journal	December 2012
Rapid Method for Coextraction of DNA and RNA from Natural Environments for Analysis of Ribosomal DNA- and rRNA-Based Microbial Community Composition Griffiths, R. I.; Whiteley, A. S.; O'Donnell, A. G. Applied and Environmental Microbiology, Vol. 66, Issue 12 https://doi.org/10.1128/aem.66.12.5488-5491.2000	journal	December 2000
Rapid genetic identification and mapping of enzymatically amplified ribosomal DNA from several Cryptococcus species. Vilgalys, R.; Hester, M. Journal of Bacteriology, Vol. 172, Issue 8 https://doi.org/10.1128/jb.172.8.4238-4246.1990	journal	August 1990
Phylogenetic identification and in situ detection of individual microbial cells without cultivation. Amann, R. I.; Ludwig, W.; Schleifer, K. H. Microbiological reviews, Vol. 59, Issue 1 https://doi.org/10.1128/mr.59.1.143-169.1995	journal	January 1995
Foliar sulfur and nitrogen along an 800-km pollution gradient Pregitzer, Kurt S.; Burton, Andrew J.; Mroz, Glenn D. Canadian Journal of Forest Research, Vol. 22, Issue 11 https://doi.org/10.1139/x92-230	journal	November 1992
The metagenomics RAST server – a public resource for the automatic phylogenetic and functional analysis of metagenomes Meyer, F.; Paarmann, D.; D'Souza, M. BMC Bioinformatics, Vol. 9, Issue 1 https://doi.org/10.1186/1471-2105-9-386	journal	September 2008
Metabolic Reconstruction for Metagenomic Data and Its Application to the Human Microbiome Abubucker, Sahar; Segata, Nicola; Goll, Johannes PLoS Computational Biology, Vol. 8, Issue 6 https://doi.org/10.1371/journal.pcbi.1002358	journal	June 2012
Simultaneous Assessment of Soil Microbial Community Structure and Function through Analysis of the Meta-Transcriptome Urich, Tim; Lanzén, Anders; Qi, Ji PLoS ONE, Vol. 3, Issue 6 https://doi.org/10.1371/journal.pone.0002527	journal	June 2008
Fungi Unearthed: Transcripts Encoding Lignocellulolytic and Chitinolytic Enzymes in Forest Soil Kellner, Harald; Vandenbol, Micheline PLoS ONE, Vol. 5, Issue 6 https://doi.org/10.1371/journal.pone.0010971	journal	June 2010
Simulated Atmospheric N Deposition Alters Fungal Community Composition and Suppresses Ligninolytic Gene Expression in a Northern Hardwood Forest Edwards, Ivan P.; Zak, Donald R.; Kellner, Harald PLoS ONE, Vol. 6, Issue 6 https://doi.org/10.1371/journal.pone.0020421	journal	June 2011
Metatranscriptomics Reveals the Diversity of Genes Expressed by Eukaryotes in Forest Soils Damon, Coralie; Lehembre, Frédéric; Oger-Desfeux, Christine PLoS ONE, Vol. 7, Issue 1 https://doi.org/10.1371/journal.pone.0028967	journal	January 2012
Nitrogen Deposition Modifies soil Carbon Storage Through Changes in Microbial Enzymatic Activity Waldrop, Mark P.; Zak, Donald R.; Sinsabaugh, Robert L. Ecological Applications, Vol. 14, Issue 4 https://doi.org/10.1890/03-5120	journal	August 2004
SIMULATED ATMOSPHERIC NO ₃ ⁻ DEPOSITION INCREASES SOIL ORGANIC MATTER BY SLOWING DECOMPOSITION Zak, Donald R.; Holmes, William E.; Burton, Andrew J. Ecological Applications, Vol. 18, Issue 8 https://doi.org/10.1890/07-1743.1	journal	December 2008
Simulated Atmospheric Nitrogen Deposition Alters Actinobacterial Community Composition in Forest Soils Eisenlord, Sarah D.; Zak, Donald R. Soil Science Society of America Journal, Vol. 74, Issue 4 https://doi.org/10.2136/sssaj2009.0240	journal	January 2010
Distribution of Extracellular Enzymes in Soils: Spatial Heterogeneity and Determining Factors at Various Scales Baldrian, Petr Soil Science Society of America Journal, Vol. 78, Issue 1 https://doi.org/10.2136/sssaj2013.04.0155dgs	journal	January 2014
Long-Term Experimental Nitrogen Deposition Alters the Composition of the Active Fungal Community in the Forest Floor Entwistle, Elizabeth M.; Zak, Donald R.; Edwards, Ivan P. Soil Science Society of America Journal, Vol. 77, Issue 5 https://doi.org/10.2136/sssaj2013.05.0179	journal	January 2013
Changes in Fungal Community Composition in Response to Elevated Atmospheric CO2 and Nitrogen Fertilization Varies with Soil Horizon Weber, Carolyn F.; Vilgalys, Rytas; Kuske, Cheryl R. Frontiers in Microbiology, Vol. 4 https://doi.org/10.3389/fmicb.2013.00078	journal	January 2013

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59 BASIC BIOLOGICAL SCIENCES
54 ENVIRONMENTAL SCIENCES
metatranscriptomics
nitrogen deposition
soil fungal community
soil bacterial community
carbohydrate active enzymes
soil RNA

Title: Forest floor community metatranscriptomes identify fungal and bacterial responses to N deposition in two maple forests

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