Structure of CPV17 polyhedrin determined by the improved analysis of serial femtosecond crystallographic data

Ginn, Helen M.; Messerschmidt, Marc; Ji, Xiaoyun; Zhang, Hanwen; Axford, Danny; Gildea, Richard J.; Winter, Graeme; Brewster, Aaron S.; Hattne, Johan; Wagner, Armin; Grimes, Jonathan M.; Evans, Gwyndaf; Sauter, Nicholas K.; Sutton, Geoff; Stuart, David I.

doi:10.1038/ncomms7435

Title: Structure of CPV17 polyhedrin determined by the improved analysis of serial femtosecond crystallographic data

Journal Article · Mon Mar 09 00:00:00 EDT 2015 · Nature Communications

DOI:https://doi.org/10.1038/ncomms7435· OSTI ID:1190163

Ginn, Helen M. ^[1]; Messerschmidt, Marc ^[2]; Ji, Xiaoyun ^[3]; Zhang, Hanwen ^[1]; Axford, Danny ^[4]; Gildea, Richard J. ^[4]; Winter, Graeme ^[4]; Brewster, Aaron S. ^[5]; Hattne, Johan ^[5]; Wagner, Armin ^[4]; Grimes, Jonathan M. ^[6]; Evans, Gwyndaf ^[4]; Sauter, Nicholas K. ^[5]; Sutton, Geoff ^[1]; Stuart, David I. ^[6]

Univ. of Oxford (United Kingdom). Division of Structural Biology, The Wellcome Trust Centre for Human Genetics.
SLAC National Accelerator Lab., Menlo Park, CA (United States)
Univ. of Oxford (United Kingdom). Division of Structural Biology, The Wellcome Trust Centre for Human Genetics; Yale School of Medicine, New Haven, CT (United States). Molecular Biophysics and Biochemistry
Harwell Science and Innovation Campus, Didcot, Oxfordshire (United Kingdom). Diamond House.
Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States). Physical Biosciences Division.
Univ. of Oxford (United Kingdom). Division of Structural Biology, The Wellcome Trust Centre for Human Genetics; Harwell Science and Innovation Campus, Didcot, Oxfordshire (United Kingdom). Diamond House.

The X-ray free-electron laser (XFEL) allows the analysis of small weakly diffracting protein crystals, but has required very many crystals to obtain good data. Here we use an XFEL to determine the room temperature atomic structure for the smallest cytoplasmic polyhedrosis virus polyhedra yet characterized, which we failed to solve at a synchrotron. These protein microcrystals, roughly a micron across, accrue within infected cells. We use a new physical model for XFEL diffraction, which better estimates the experimental signal, delivering a high-resolution XFEL structure (1.75 Å), using fewer crystals than previously required for this resolution. The crystal lattice and protein core are conserved compared with a polyhedrin with less than 10% sequence identity. We explain how the conserved biological phenotype, the crystal lattice, is maintained in the face of extreme environmental challenge and massive evolutionary divergence. Our improved methods should open up more challenging biological samples to XFEL analysis.

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Research Organization:: SLAC National Accelerator Laboratory (SLAC), Menlo Park, CA (United States)

Sponsoring Organization:: USDOE Office of Science (SC)

OSTI ID:: 1190163

Journal Information:: Nature Communications, Vol. 6; Related Information: Portions of this research were carried out at the Linac Coherent Light Source (LCLS) at the SLAC National Accelerator Laboratory. LCLS is an Office of Science User Facility operated for the U.S. Department of Energy Office of Science by Stanford University.; ISSN 2041-1723

Publisher:: Nature Publishing GroupCopyright Statement

Country of Publication:: United States

Language:: English

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Cited by: 51 works

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Title: Structure of CPV17 polyhedrin determined by the improved analysis of serial femtosecond crystallographic data

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