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Title: Human Cytochrome P450 21A2, the Major Steroid 21-Hydroxylase: Structure of the Enzyme Progesterone Substrate Complex and Rate-Limiting C-H Bond Cleavage

Journal Article · · J. Biol. Chem.
; ; ; ; ; ; ;  [1]
  1. Michigan-Med
+ Show Author Affiliations

Research Organization:
Argonne National Laboratory (ANL), Argonne, IL (United States). Advanced Photon Source (APS)
Sponsoring Organization:
National Institutes of Health (NIH)
OSTI ID:
1188831
Journal Information:
J. Biol. Chem., Vol. 290, Issue (21) ; 05, 2015
Country of Publication:
United States
Language:
ENGLISH
Citation Metrics:
Cited by: 57 works
Citation information provided by
Web of Science

References (43)

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The new CCP 4 Coordinate Library as a toolkit for the design of coordinate-related applications in protein crystallography journal November 2004
Structure-phenotype correlations of human CYP21A2 mutations in congenital adrenal hyperplasia journal January 2013
Results of Screening 1.9 Million Texas Newborns for 21-Hydroxylase-Deficient Congenital Adrenal Hyperplasia journal April 1998
Why Human Cytochrome P450c21 Is a Progesterone 21-Hydroxylase journal May 2011
Cytochrome P450 7A1 Cholesterol 7α-Hydroxylation: INDIVIDUAL REACTION STEPS IN THE CATALYTIC CYCLE AND RATE-LIMITING FERRIC IRON REDUCTION journal December 2010
Functional Analysis of Four CYP21 Mutations from Spanish Patients with Congenital Adrenal Hyperplasia journal September 1999
Inhibition of CYP21A2 Enzyme Activity Caused by Novel Missense Mutations Identified in Brazilian and Scandinavian Patients journal June 2008
Cytochrome P450 2D Catalyze Steroid 21-Hydroxylation in the Brain journal February 2004
The enantiomer of progesterone (ent-progesterone) is a competitive inhibitor of human cytochromes P450c17 and P450c21 journal January 2003
Two genes encoding steroid 21-hydroxylase are located near the genes encoding the fourth component of complement in man. journal February 1985
Congenital Adrenal Hyperplasia due to 21-Hydroxylase Deficiency journal June 2000
Molecular Characterization of 25 Chinese Pedigrees with 21-Hydroxylase Deficiency journal March 2011
The CCP4 suite programs for protein crystallography journal September 1994
Identification of rabbit microsomal cytochrome P-450 isozyme, form 1, as a hepatic progesterone 21-hydroxylase journal March 1982
Role of the Alanine at Position 363 of Cytochrome P450 2B2 in Influencing the NADPH- and Hydroperoxide-Supported Activities journal February 1998
UCSF Chimera?A visualization system for exploratory research and analysis journal January 2004
Structural Diversity of Eukaryotic Membrane Cytochrome P450s journal April 2013
The purification and application of biologically active recombinant steroid cytochrome P450 21-hydroxylase: The major autoantigen in autoimmune Addison's disease journal August 2009
Molecular characterization of the HLA-linked steroid 21-hydroxylase B gene from an individual with congenital adrenal hyperplasia. journal June 1987
Human Cytochrome P450 Enzymes book January 2015
Phenotype-Genotype Correlations of 13 Rare CYP21A2 Mutations Detected in 46 Patients Affected with 21-Hydroxylase Deficiency and in One Carrier journal March 2010
Rate-Limiting Steps in Oxidations Catalyzed by Rabbit Cytochrome P450 1A2 † journal August 2004
Epoxidation Activities of Human Cytochromes P450c17 and P450c21 journal November 2014
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Expression of Modified Human Cytochrome P450 1A2 in Escherichia coli: Stabilization, Purification, Spectral Characterization, and Catalytic Activities of the Enzyme journal February 1994
REFMAC 5 for the refinement of macromolecular crystal structures journal March 2011
A Novel Missense Mutation, GLY424SER, in Brazilian Patients with 21-Hydroxylase Deficiency journal August 1999
The Molecular Biology, Biochemistry, and Physiology of Human Steroidogenesis and Its Disorders journal February 2011
Three-dimensional Structure of Steroid 21-Hydroxylase (Cytochrome P450 21A2) with Two Substrates Reveals Locations of Disease-associated Variants journal January 2012
Fast, scalable generation of high‐quality protein multiple sequence alignments using Clustal Omega journal January 2011
Molecular replacement with MOLREP journal December 2009
A Universal Approach to the Expression of Human and Rabbit Cytochrome P450s of the 2C Subfamily inEscherichia coli journal October 1995
Program DYNAFIT for the Analysis of Enzyme Kinetic Data: Application to HIV Proteinase journal June 1996
Minor Activities and Transition State Properties of the Human Steroid Hydroxylases Cytochromes P450c17 and P450c21, from Reactions Observed with Deuterium-Labeled Substrates journal August 2012
Complete nucleotide sequence of two steroid 21-hydroxylase genes tandemly arranged in human chromosome: a pseudogene and a genuine gene. journal May 1986
The Rate-determining Step in P450 C21-catalyzing Reactions in a Membrane-reconstituted System journal January 2001
Multiparametric scaling of diffraction intensities journal April 2003
Purification and characterization of bovine steroid 21-hydroxylase (P450c21) efficiently expressed in Escherichia coli journal May 2006
Explanation of Pre-Steady-State Kinetics and Decreased Burst Amplitude of HIV-1 Reverse Transcriptase at Sites of Modified DNA Bases with an Additional, Nonproductive Enzyme−DNA−Nucleotide Complex † journal April 1999
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Cited By (13)

In Situ Proteolysis for Crystallization of Membrane Bound Cytochrome P450 17A1 and 17A2 Proteins from Zebrafish journal April 2016
Genotype–phenotype correlation study and mutational and hormonal analysis in a Chinese cohort with 21‐hydroxylase deficiency journal April 2019
Detection of a novel severe mutation affecting the CYP21A2 gene in a Chilean male with salt wasting congenital adrenal hyperplasia journal September 2019
Congenital Adrenal Hyperplasia (CAH) due to 21-Hydroxylase Deficiency: A Comprehensive Focus on 233 Pathogenic Variants of CYP21A2 Gene journal February 2018
Epidemiology, pathogenesis, and diagnosis of Addison’s disease in adults journal July 2019
Instability of the Human Cytochrome P450 Reductase A287P Variant Is the Major Contributor to Its Antley-Bixler Syndrome-like Phenotype journal September 2016
Conformational selection dominates binding of steroids to human cytochrome P450 17A1 journal June 2019
Human cytochrome P450 enzymes bind drugs and other substrates mainly through conformational-selection modes journal July 2019
Measuring the structural impact of mutations on cytochrome P450 21A2, the major steroid 21-hydroxylase related to congenital adrenal hyperplasia journal April 2019
Bioactivity of Curcumin on the Cytochrome P450 Enzymes of the Steroidogenic Pathway posted_content August 2019
Bioactivity of Curcumin on the Cytochrome P450 Enzymes of the Steroidogenic Pathway. text January 2019
Bioactivity of Curcumin on the Cytochrome P450 Enzymes of the Steroidogenic Pathway journal September 2019
Bioactivity of curcumin on the cytochrome P450 enzymes of the steroidogenic pathway posted_content August 2019

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