Re-engineering bacteria for ethanol production

Yomano, Lorraine P; York, Sean W; Zhou, Shengde; Shanmugam, Keelnatham; Ingram, Lonnie O

Title: Re-engineering bacteria for ethanol production

Patent · Tue May 06 00:00:00 EDT 2014

OSTI ID:1130528

Yomano, Lorraine P; York, Sean W; Zhou, Shengde; Shanmugam, Keelnatham; Ingram, Lonnie O

The invention provides recombinant bacteria, which comprise a full complement of heterologous ethanol production genes. Expression of the full complement of heterologous ethanol production genes causes the recombinant bacteria to produce ethanol as the primary fermentation product when grown in mineral salts medium, without the addition of complex nutrients. Methods for producing the recombinant bacteria and methods for producing ethanol using the recombinant bacteria are also disclosed.

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Research Organization:: University of Florida, Gainesville, FL, USA

Sponsoring Organization:: USDOE

DOE Contract Number:: FG02-96ER20222

Assignee:: University of Florida Research Foundation, Inc. (Gainesville, FL)

Patent Number(s):: 8,716,002

Application Number:: 12/375,484

OSTI ID:: 1130528

Country of Publication:: United States

Language:: English

References (14)

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Gene Integration and Expression and Extracellular Secretion of Erwinia chrysanthemi Endoglucanase CelY (celY) and CelZ (celZ) in Ethanologenic Klebsiella oxytoca P2 Zhou, S.; Davis, F. C.; Ingram, L. O. Applied and Environmental Microbiology, Vol. 67, Issue 1 https://doi.org/10.1128/AEM.67.1.6-14.2001	journal	January 2001
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Ribose Utilization with an Excess of Mutarotase Causes Cell Death Due to Accumulation of Methylglyoxal Kim, Insook; Kim, Eunjung; Yoo, Seokho Journal of Bacteriology, Vol. 186, Issue 21 https://doi.org/10.1128/JB.186.21.7229-7235.2004	journal	November 2004
Re-engineering Escherichia coli for ethanol production Yomano, L. P.; York, S. W.; Zhou, S. Biotechnology Letters, Vol. 30, Issue 12 https://doi.org/10.1007/s10529-008-9821-3	journal	September 2008
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Ethanol production in Gram-positive microbes Ingram, Lonnie O'Neal; Barbosa-Alleyne, Maria D. F. https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/5482846 US Patent Document 5,482,846	patent	January 1996
Ethanol production in gram-positive microbes Ingram, Lonnie O'Neal; Barbosa-Alleyne, Maria D. F. https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/5916787 US Patent Document 5,916,787	patent	June 1999
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Lack of Protective Osmolytes Limits Final Cell Density and Volumetric Productivity of Ethanologenic Escherichia coli KO11 during Xylose Fermentation Underwood, S. A.; Buszko, M. L.; Shanmugam, K. T. Applied and Environmental Microbiology, Vol. 70, Issue 5 https://doi.org/10.1128/AEM.70.5.2734-2740.2004	journal	May 2004
Molecular cloning of the carboxylesterase gene and biochemical characterization of the encoded protein from Pseudomonas citronellolis ATCC 13674 Chao, Yun-Peng; Fu, Hongyong; Wang, Yu-Ling Research in Microbiology, Vol. 154, Issue 7 https://doi.org/10.1016/S0923-2508(03)00144-X	journal	September 2003
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