High Resolution Magic Angle Spinning 1H-NMR Metabolic Profiling of Nanoliter Biological Tissues at High Magnetic Field
Journal Article
·
· Chinese Journal of Magnetic Resonance, 30(1):1-11
OSTI ID:1080137
It is demonstrated that a high resolution magic angle spinning 1H-NMR spectrum of biological tissue samples with volumes as small as 150 nanoliters, or 0.15 mg in weight, can be acquired in a few minutes at 21.1 T magnetic field using a commercial 1.6 mm fast-MAS probe with minor modification of the MAS rotor. The strategies of sealing the samples inside the MAS rotor to avoid fluid leakage as well as the ways of optimizing the signal to noise are discussed.
- Research Organization:
- Pacific Northwest National Lab. (PNNL), Richland, WA (United States). Environmental Molecular Sciences Lab. (EMSL)
- Sponsoring Organization:
- USDOE
- DOE Contract Number:
- AC05-76RL01830
- OSTI ID:
- 1080137
- Report Number(s):
- PNNL-SA-89007; 42296; 400412000
- Journal Information:
- Chinese Journal of Magnetic Resonance, 30(1):1-11, Journal Name: Chinese Journal of Magnetic Resonance, 30(1):1-11
- Country of Publication:
- United States
- Language:
- English
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