The Deinococcus radiodurans DR1245 Protein, a DdrB Partner Homologous to YbjN Proteins and Reminiscent of Type III Secretion System Chaperones

Journal Article · Mon Feb 18 00:00:00 EST 2013 · PLoS ONE

DOI:https://doi.org/10.1371/journal.pone.0056558· OSTI ID:1072760

Norais, Cédric ^[1]; Servant, Pascale ^[2]; Bouthier-de-la-Tour, Claire ^[2]; Coureux, Pierre-Damien ^[3]; Ithurbide, Solenne ^[2]; Vannier, Françoise ^[2]; Guerin, Philippe P. ^[4]; Dulberger, Charles L. ^[5]; Satyshur, Kenneth A. ^[5]; Keck, James L. ^[5]; Armengaud, Jean ^[4]; Cox, Michael M. ^[5]; Sommer, Suzanne ^[2]

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1. Univ. of Wisconsin, Madison, WI (United States); Ecole Polytechnic, Paaiseau (France)
2. Univ. of Paris-Sud, Orsay (France); National Center of Scientific Research, Orsay (France)
3. Ecole Polytechnic, Paaiseau (France)
4. French Alternatives Energy Commission (France)
5. Univ. of Wisconsin, Madison, WI (United States)

The bacterium Deinococcus radiodurans exhibits an extreme resistance to ionizing radiation. A small subset of Deinococcus genus-specific genes were shown to be up-regulated upon exposure to ionizing radiation and to play a role in genome reconstitution. These genes include an SSB-like protein called DdrB. Here, we identified a novel protein encoded by the dr1245gene as an interacting partner of DdrB. A strain devoid of the DR1245 protein is impaired in growth, exhibiting a generation time approximately threefold that of the wild type strain while radioresistance is not affected. We determined the three-dimensional structure of DR1245, revealing a relationship with type III secretion system chaperones and YbjN family proteins. Thus, DR1245 may display some chaperone activity towards DdrB and possibly other substrates.

• Research Organization: Argonne National Lab. (ANL), Argonne, IL (United States). Advanced Photon Source (APS)
• Sponsoring Organization: FOREIGNNIH
• DOE Contract Number: AC02-06CH11357
• OSTI ID: 1072760
• Journal Information: PLoS ONE, Vol. 8, Issue (2) ; 02, 2013; ISSN 1932-6203
• Publisher: Public Library of Science
• Country of Publication: United States
• Language: ENGLISH

Related Subjects

59 BASIC BIOLOGICAL SCIENCES