Fluorescence Axial Localization with Nanometer Accuracy and Precision

Journal Article · Fri Jun 15 00:00:00 EDT 2012 · Nano Letters

DOI:https://doi.org/10.1021/nl301542c· OSTI ID:1052066

Li, Hui; Yen, Chi-Fu; Sivasankar, Sanjeevi

We describe a new technique, standing wave axial nanometry (SWAN), to image the axial location of a single nanoscale fluorescent object with sub-nanometer accuracy and 3.7 nm precision. A standing wave, generated by positioning an atomic force microscope tip over a focused laser beam, is used to excite fluorescence; axial position is determined from the phase of the emission intensity. We use SWAN to measure the orientation of single DNA molecules of different lengths, grafted on surfaces with different functionalities.

• Research Organization: Ames Lab., Ames, IA (United States)
• Sponsoring Organization: USDOE Office of Science (SC)
• DOE Contract Number: DE-AC02-07CH11358
• OSTI ID: 1052066
• Report Number(s): IS-J 7757
• Journal Information: Nano Letters, Vol. 12, Issue 7
• Country of Publication: United States
• Language: English

Related Subjects

36 MATERIALS SCIENCE