The Genome Sequence of Methanohalophilus mahii SLPT Reveals Differences in the Energy Metabolism among Members of the Methanosarcinaceae Inhabiting Freshwater and Saline Environments

Journal Article · Wed Dec 01 00:00:00 EST 2010 · Archaea

OSTI ID:1007843

Spring, Stefan ^[1]; Scheuner, Carmen ^[1]; Lapidus, Alla L ^[2]; Lucas, Susan ^[2]; Glavina Del Rio, Tijana ^[2]; Tice, Hope ^[2]; Copeland, A ^[3]; Cheng, Jan-Fang ^[2]; Chen, Feng ^[2]; Nolan, Matt ^[2]; Saunders, Elizabeth H ^[4]; Pitluck, Samuel ^[5]; Liolios, Konstantinos ^[2]; Ivanova, N ^[3]; Mavromatis, K ^[3]; Lykidis, A ^[3]; Pati, Amrita ^[3]; Chen, Amy ^[2]; Palaniappan, Krishna ^[2]; Land, Miriam L ^[5] ; Hauser, Loren John ^[5]; Chang, Yun-Juan ^[5]; Jeffries, Cynthia D ^[5]; Goodwin, Lynne A ^[4]; Detter, J Chris ^[2]; Brettin, Thomas S ^[5]; Rohde, Manfred ^[6]; Goker, Markus ^[1]; Woyke, Tanja ^[5]; Bristow, James ^[2]; Eisen, Jonathan ^[2]; Markowitz, Victor ^[2]; Hugenholtz, Philip ^[3]; Kyrpidis, Nikos C ^[5]; Klenk, Hans-Peter ^[1]

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1. DSMZ - German Collection of Microorganisms and Cell Cultures GmbH, Braunschweig, Germany
2. Joint Genome Institute, Walnut Creek, California
3. U.S. Department of Energy, Joint Genome Institute
4. Los Alamos National Laboratory (LANL)
5. ORNL
6. HZI - Helmholtz Centre for Infection Research, Braunschweig, Germany

Methanohalophilus mahii is the type species of the genus Methanohalophilus, which currently comprises three distinct species with validly published names. Mhp. mahii represents moderately halophilic methanogenic archaea with a strictly methylotrophic metabolism. The type strain SLPT was isolated from hypersaline sediments collected from the southern arm of Great Salt Lake, Utah. Here we describe the features of this organism, together with the complete genome sequence and annotation. The 2,012,424 bp genome is a single replicon with 2032 protein-coding and 63 RNA genes and part of the Genomic Encyclopedia of Bacteria and Archaea project. A comparison of the reconstructed energy metabolism in the halophilic species Mhp. mahii with other representatives of the Methanosarcinaceae reveals some interesting differences to freshwater species.

• Research Organization: Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
• Sponsoring Organization: Work for Others (WFO)
• DOE Contract Number: DE-AC05-00OR22725
• OSTI ID: 1007843
• Journal Information: Archaea, Vol. 2010
• Country of Publication: United States
• Language: English