Rhamnogalacturonan I is a recalcitrant pectin domain during Clostridium thermocellum-mediated deconstruction of switchgrass biomass

Biswal, Ajaya K.; Hengge, Neal N.; Atmodjo, Melani A.; Abraham, Paul E.; Engle, Nancy L.; Mohanty, Sushree S.; Black, Ian M.; Cheng, Xiaochun; Ryno, David; Azadi, Parastoo; Tschaplinski, Timothy J.; Bomble, Yannick J.; Mohnen, Debra

doi:10.1186/s13068-025-02703-9

Title: Rhamnogalacturonan I is a recalcitrant pectin domain during Clostridium thermocellum-mediated deconstruction of switchgrass biomass

Journal Article · Mon Oct 27 00:00:00 UTC 2025 · Biotechnology for Biofuels and Bioproducts

DOI: https://doi.org/10.1186/s13068-025-02703-9 · OSTI ID:3016257

Biswal, Ajaya K. ^[1]; Hengge, Neal N. ^[2]; Atmodjo, Melani A. ^[1];

^[3];

^[3]; Mohanty, Sushree S. ^[1]; Black, Ian M. ^[4]; Cheng, Xiaochun ^[5]; Ryno, David ^[1]; Azadi, Parastoo ^[4];

^[3]; Bomble, Yannick J. ^[2]; Mohnen, Debra ^[1]

Univ. of Georgia, Athens, GA (United States); Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States)
National Renewable Energy Laboratory (NREL), Golden, CO (United States); Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States)
Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States)
Univ. of Georgia, Athens, GA (United States)
Univ. of Georgia, Athens, GA (United States); Duke Univ., Durham, NC (United States)

Background Liquid fuels from lignocellulosic feedstocks are required for transition to a sustainable bioeconomy. However, the recalcitrance of carbon-containing feedstock cell walls to deconstruction poses a barrier to cost effective biological conversion of plant biomass to biofuels. One-step consolidated bioprocessing (CBP) in which anaerobic thermophilic bacteria convert lignocellulosic biomass into liquid fuels is a platform for overcoming the recalcitrance of plant biomass. Results The amounts of hemicellulosic and pectic polysaccharides, two complex cell wall glycans that contribute to plant biomass recalcitrance and that are partially solubilized during CBP of switchgrass aerial biomass by Clostridium thermocellum were evaluated in the liquor, solid residues and residue washate recovered during a 120-h CBP process. After 120 h, 24% of milled switchgrass was solubilized in the C. thermocellum CBP platform. Higher concentrations of arabinose, xylose, galactose, and glucose accumulated in the CBP-fermentation liquor and washate compared to fermentation controls without C. thermocellum, indicating that C. thermocellum solubilized hemicelluloses, but did not fully metabolize them. After five days of fermentation, the relative amount of rhamnose in the solid residues increased by 16% compared to controls, and CBP solid residues had more than 23% increased reactivity against RG-I reactive monoclonal antibodies, indicating that the pectic polymer rhamnogalacturonan I (RG-I) was not effectively solubilized from switchgrass biomass by C. thermocellum CBP. Similarly, the amount of mannose (Man) in the CBP solid residues increased by 7% and reactivity against galactomannan reactive antibodies increased by greater than 14%, indicating that the hemicellulosic polymer galactomannan was also resistant to degradation by C. thermocellum during CBP fermentation. Conclusions These findings show that C. thermocellum is unable to effectively degrade RG-I pectic and galactomannan hemicellulosic components in switchgrass biomass. Targeting these polymers for improved solubilization could enhance the efficiency of conversion of grass biomass to biofuels.

View Accepted Manuscript (DOE)

Cite

Export

Save

Research Organization:: National Laboratory of the Rockies (NLR), Golden, CO (United States); Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States); Univ. of Georgia, Athens, GA (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Basic Energy Sciences (BES); USDOE Office of Science (SC), Biological and Environmental Research (BER)

Grant/Contract Number:: AC05-00OR22725; AC36-08GO28308; SC0015662

OSTI ID:: 3016257

Report Number(s):: NLR/JA--2700-99119

Journal Information:: Biotechnology for Biofuels and Bioproducts, Journal Name: Biotechnology for Biofuels and Bioproducts Journal Issue: 1 Vol. 18; ISSN 2731-3654

Publisher:: BioMed CentralCopyright Statement

Country of Publication:: United States

Language:: English

References (80)

Xylan and xylan derivatives - biopolymers with valuable properties, 1. Naturally occurring xylans structures, isolation procedures and properties Ebringerová, Anna; Heinze, Thomas Macromolecular Rapid Communications, Vol. 21, Issue 9 https://doi.org/10.1002/1521-3927(20000601)21:9<542::AID-MARC542>3.0.CO;2-7	journal	June 2000
Strong cellulase inhibition by Mannan polysaccharides in cellulose conversion to sugars: Strong Cellulase Inhibition by Heteromannans Kumar, Rajeev; Wyman, Charles E. Biotechnology and Bioengineering, Vol. 111, Issue 7 https://doi.org/10.1002/bit.25218	journal	May 2014
Automated Synthesis of Arabinoxylan-Oligosaccharides Enables Characterization of Antibodies that Recognize Plant Cell Wall Glycans Schmidt, Deborah; Schuhmacher, Frank; Geissner, Andreas Chemistry - A European Journal, Vol. 21, Issue 15 https://doi.org/10.1002/chem.201500065	journal	February 2015
A cellulosomal double‐dockerin module from Clostridium thermocellum shows distinct structural and cohesin‐binding features Chen, Chao; Yang, Hongwu; Dong, Sheng Protein Science, Vol. 33, Issue 4 https://doi.org/10.1002/pro.4937	journal	March 2024
From cellulosomes to cellulosomics Bayer, Edward A.; Lamed, Raphael; White, Bryan A. The Chemical Record, Vol. 8, Issue 6, p. 364-377 https://doi.org/10.1002/tcr.20160	journal	January 2008
Pectinolytic activity ofClostridium thermocellum: Its use for anaerobic fermentation of sugar beet pulp Spinnler, H. E.; Lavigne, B.; Blachere, H. Applied Microbiology and Biotechnology, Vol. 23, Issue 6 https://doi.org/10.1007/BF02346055	journal	March 1986
A defined growth medium with very low background carbon for culturing Clostridium thermocellum Holwerda, Evert K.; Hirst, Kyle D.; Lynd, Lee R. Journal of Industrial Microbiology & Biotechnology, Vol. 39, Issue 6 https://doi.org/10.1007/s10295-012-1091-3	journal	February 2012
A New Member of Family 11 Polysaccharide Lyase, Rhamnogalacturonan Lyase (CtRGLf) from Clostridium thermocellum Dhillon, Arun; Fernandes, Vania O.; Dias, Fernando M. V. Molecular Biotechnology, Vol. 58, Issue 4 https://doi.org/10.1007/s12033-016-9921-6	journal	February 2016
Enzymic degradation of sorghum glucuronoarabinoxylans leading to tentative structures Verbruggen, Marian A.; Beldman, Gerrit; Voragen, Alphons G. J. Carbohydrate Research, Vol. 306, Issue 1-2 https://doi.org/10.1016/S0008-6215(97)10065-9	journal	January 1998
Carbohydrate analysis by a phenol–sulfuric acid method in microplate format Masuko, Tatsuya; Minami, Akio; Iwasaki, Norimasa Analytical Biochemistry, Vol. 339, Issue 1 https://doi.org/10.1016/j.ab.2004.12.001	journal	April 2005
The multi-ligand binding first family 35 Carbohydrate Binding Module (CBM35) of Clostridium thermocellum targets rhamnogalacturonan I Dhillon, Arun; Sharma, Kedar; Rajulapati, Vikky Archives of Biochemistry and Biophysics, Vol. 654 https://doi.org/10.1016/j.abb.2018.07.023	journal	September 2018
Chemical compositions of four switchgrass populations Yan, Jinhua; Hu, Zhoujian; Pu, Yunqiao Biomass and Bioenergy, Vol. 34, Issue 1, p. 48-53 https://doi.org/10.1016/j.biombioe.2009.09.010	journal	January 2010
Characterization of reduced carbohydrate solubilization during Clostridium thermocellum fermentation with high switchgrass concentrations Shao, Xiongjun; Murphy, Sean J.; Lynd, Lee R. Biomass and Bioenergy, Vol. 139 https://doi.org/10.1016/j.biombioe.2020.105623	journal	August 2020
Chemical profiles of switchgrass Hu, Zhoujian; Sykes, Robert; Davis, Mark F. Bioresource Technology, Vol. 101, Issue 9 https://doi.org/10.1016/j.biortech.2009.12.033	journal	May 2010
Closing the carbon balance for fermentation by Clostridium thermocellum (ATCC 27405) Ellis, Lucas D.; Holwerda, Evert K.; Hogsett, David Bioresource Technology, Vol. 103, Issue 1 https://doi.org/10.1016/j.biortech.2011.09.128	journal	January 2012
Three novel rhamnogalacturonan I- pectins degrading enzymes from Aspergillus aculeatinus: Biochemical characterization and application potential Lemaire, Adrien; Duran Garzon, Catalina; Perrin, Aurore Carbohydrate Polymers, Vol. 248 https://doi.org/10.1016/j.carbpol.2020.116752	journal	November 2020
Structure and functionality of Rhamnogalacturonan I in the cell wall and in solution: A review Kaczmarska, Adrianna; Pieczywek, Piotr M.; Cybulska, Justyna Carbohydrate Polymers, Vol. 278 https://doi.org/10.1016/j.carbpol.2021.118909	journal	February 2022
Recent progress in consolidated bioprocessing Olson, Daniel G.; McBride, John E.; Joe Shaw, A. Current Opinion in Biotechnology, Vol. 23, Issue 3, p. 396-405 https://doi.org/10.1016/j.copbio.2011.11.026	journal	June 2012
Research advances on the consolidated bioprocessing of lignocellulosic biomass Li, Zhongye; Waghmare, Pankajkumar R.; Dijkhuizen, Lubbert Engineering Microbiology, Vol. 4, Issue 2 https://doi.org/10.1016/j.engmic.2024.100139	journal	June 2024
Assessment of the pectin degrading enzyme network of Aspergillus niger by functional genomics Martens-Uzunova, Elena S.; Schaap, Peter J. Fungal Genetics and Biology, Vol. 46, Issue 1 https://doi.org/10.1016/j.fgb.2008.07.021	journal	March 2009
Combustion improvements of upgraded biomass by washing and torrefaction Abelha, Pedro; Mourão Vilela, Carlos; Nanou, Pavlina Fuel, Vol. 253 https://doi.org/10.1016/j.fuel.2019.05.050	journal	October 2019
Release, Recycle, Rebuild: Cell-Wall Remodeling, Autodegradation, and Sugar Salvage for New Wall Biosynthesis during Plant Development Barnes, William J.; Anderson, Charles T. Molecular Plant, Vol. 11, Issue 1 https://doi.org/10.1016/j.molp.2017.08.011	journal	January 2018
E/Z-Thesinine-O-4′-α-rhamnoside, pyrrolizidine conjugates produced by grasses (Poaceae) Koulman, Albert; Seeliger, Claudine; Edwards, Patrick J. B. Phytochemistry, Vol. 69, Issue 9 https://doi.org/10.1016/j.phytochem.2008.03.017	journal	June 2008
Rhamnose in plants - from biosynthesis to diverse functions Jiang, Nan; Dillon, Francisco M.; Silva, Alexander Plant Science, Vol. 302 https://doi.org/10.1016/j.plantsci.2020.110687	journal	January 2021
Engineering the cellulolytic bacterium, Clostridium thermocellum, to co-utilize hemicellulose Chou, Katherine J.; Croft, Trevor; Hebdon, Skyler D. Metabolic Engineering, Vol. 83, p. 193-205 https://doi.org/10.1016/j.ymben.2024.03.008	journal	May 2024
Skyline for Small Molecules: A Unifying Software Package for Quantitative Metabolomics Adams, Kendra J.; Pratt, Brian; Bose, Neelanjan Journal of Proteome Research, Vol. 19, Issue 4 https://doi.org/10.1021/acs.jproteome.9b00640	journal	January 2020
Comparative Study on Pretreatment Processes for Different Utilization Purposes of Switchgrass Wang, Fan; Shi, Dongxiang; Han, Ju ACS Omega, Vol. 5, Issue 35 https://doi.org/10.1021/acsomega.0c01047	journal	August 2020
Elucidating the Structural Changes to Populus Lignin during Consolidated Bioprocessing with Clostridium thermocellum Akinosho, Hannah O.; Yoo, Chang Geun; Dumitrache, Alexandru ACS Sustainable Chemistry & Engineering, Vol. 5, Issue 9 https://doi.org/10.1021/acssuschemeng.7b01203	journal	July 2017
Quantitative Saccharification of Wood and Cellulose Saeman, Jerome F.; Bubl, Janet L.; Harris, Elwin E. Industrial & Engineering Chemistry Analytical Edition, Vol. 17, Issue 1, p. 35-37 https://doi.org/10.1021/i560137a008	journal	January 1945
A Colorimetric Method for the Determination of Sugars Dubois, M.; Gilles, K.; Hamilton, J. K. Nature, Vol. 168, Issue 4265 https://doi.org/10.1038/168167a0	journal	July 1951
Sugar release and growth of biofuel crops are improved by downregulation of pectin biosynthesis Biswal, Ajaya K.; Atmodjo, Melani A.; Li, Mi Nature Biotechnology, Vol. 36, Issue 3 https://doi.org/10.1038/nbt.4067	journal	February 2018
Development of a thermophilic coculture for corn fiber conversion to ethanol Beri, Dhananjay; York, William S.; Lynd, Lee R. Nature Communications, Vol. 11, Issue 1 https://doi.org/10.1038/s41467-020-15704-z	journal	April 2020
Metaproteomics reveals enzymatic strategies deployed by anaerobic microbiomes to maintain lignocellulose deconstruction at high solids Chirania, Payal; Holwerda, Evert K.; Giannone, Richard J. Nature Communications, Vol. 13, Issue 1 https://doi.org/10.1038/s41467-022-31433-x	journal	July 2022
Advances in lignocellulosic feedstocks for bioenergy and bioproducts Sulis, Daniel B.; Lavoine, Nathalie; Sederoff, Heike Nature Communications, Vol. 16, Issue 1 https://doi.org/10.1038/s41467-025-56472-y	journal	February 2025
SIRIUS 4: a rapid tool for turning tandem mass spectra into metabolite structure information Dührkop, Kai; Fleischauer, Markus; Ludwig, Marcus Nature Methods, Vol. 16, Issue 4 https://doi.org/10.1038/s41592-019-0344-8	journal	March 2019
Transcriptomic analysis of a Clostridium thermocellum strain engineered to utilize xylose: responses to xylose versus cellobiose feeding Tafur Rangel, Albert E.; Croft, Trevor; González Barrios, Andrés Fernando Scientific Reports, Vol. 10, Issue 1 https://doi.org/10.1038/s41598-020-71428-6	journal	September 2020
Enzymatic diversity of the Clostridium thermocellum cellulosome is crucial for the degradation of crystalline cellulose and plant biomass Hirano, Katsuaki; Kurosaki, Masahiro; Nihei, Satoshi Scientific Reports, Vol. 6, Issue 1 https://doi.org/10.1038/srep35709	journal	October 2016
Pentose sugars inhibit metabolism and increase expression of an AgrD-type cyclic pentapeptide in Clostridium thermocellum Verbeke, Tobin J.; Giannone, Richard J.; Klingeman, Dawn M. Scientific Reports, Vol. 7, Issue 1 https://doi.org/10.1038/srep43355	journal	February 2017
Lignocellulose fermentation and residual solids characterization for senescent switchgrass fermentation by Clostridium thermocellum in the presence and absence of continuous in situ ball-milling Balch, Michael L.; Holwerda, Evert K.; Davis, Mark F. Energy & Environmental Science, Vol. 10, Issue 5 https://doi.org/10.1039/C6EE03748H	journal	January 2017
The effect of switchgrass plant cell wall properties on its deconstruction by thermochemical pretreatments coupled with fungal enzymatic hydrolysis or Clostridium thermocellum consolidated bioprocessing Kothari, Ninad; Bhagia, Samarthya; Pu, Yunqiao Green Chemistry, Vol. 22, Issue 22 https://doi.org/10.1039/D0GC02546A	journal	January 2020
Lignin content in natural Populus variants affects sugar release Studer, M. H.; DeMartini, J. D.; Davis, M. F. Proceedings of the National Academy of Sciences, Vol. 108, Issue 15, p. 6300-6305 https://doi.org/10.1073/pnas.1009252108	journal	March 2011
The biomass distribution on Earth Bar-On, Yinon M.; Phillips, Rob; Milo, Ron Proceedings of the National Academy of Sciences, Vol. 115, Issue 25 https://doi.org/10.1073/pnas.1711842115	journal	May 2018
A family 26 mannanase produced by Clostridium thermocellum as a component of the cellulosome contains a domain which is conserved in mannanases from anaerobic fungi Vercoe, Philip E.; Halstead, Jonathan R.; Hazlewood, Geoffrey P. Microbiology, Vol. 145, Issue 11 https://doi.org/10.1099/00221287-145-11-3101	journal	November 1999
The Effects on Lignin Structure of Overexpression of Ferulate 5-Hydroxylase in Hybrid Poplar ¹ Stewart, Jaclyn J.; Akiyama, Takuya; Chapple, Clint Plant Physiology, Vol. 150, Issue 2 https://doi.org/10.1104/pp.109.137059	journal	April 2009
A Comprehensive Toolkit of Plant Cell Wall Glycan-Directed Monoclonal Antibodies Pattathil, S.; Avci, U.; Baldwin, D. Plant Physiology, Vol. 153, Issue 2, p. 514-525 https://doi.org/10.1104/pp.109.151985	journal	April 2010
A Synthetic Glycan Microarray Enables Epitope Mapping of Plant Cell Wall Glycan-Directed Antibodies Ruprecht, Colin; Bartetzko, Max P.; Senf, Deborah Plant Physiology, Vol. 175, Issue 3 https://doi.org/10.1104/pp.17.00737	journal	September 2017
The Arabidopsis irregular xylem8 Mutant Is Deficient in Glucuronoxylan and Homogalacturonan, Which Are Essential for Secondary Cell Wall Integrity Persson, Staffan; Caffall, Kerry Hosmer; Freshour, Glenn The Plant Cell, Vol. 19, Issue 1 https://doi.org/10.1105/tpc.106.047720	journal	January 2007
Rhamnogalacturonan‐I is a determinant of cell–cell adhesion in poplar wood Yang, Haibing; Benatti, Matheus R.; Karve, Rucha A. Plant Biotechnology Journal, Vol. 18, Issue 4 https://doi.org/10.1111/pbi.13271	journal	October 2019
Dramatic performance of Clostridium thermocellum explained by its wide range of cellulase modalities Xu, Qi; Resch, Michael G.; Podkaminer, Kara Science Advances, Vol. 2, Issue 2 https://doi.org/10.1126/sciadv.1501254	journal	February 2016
Microbial Cellulose Utilization: Fundamentals and Biotechnology Lynd, L. R.; Weimer, P. J.; van Zyl, W. H. Microbiology and Molecular Biology Reviews, Vol. 66, Issue 3, p. 506-577 https://doi.org/10.1128/MMBR.66.3.506-577.2002	journal	September 2002
Characterization of a cellulose-binding, cellulase-containing complex in Clostridium thermocellum. Lamed, R.; Setter, E.; Bayer, E. A. Journal of Bacteriology, Vol. 156, Issue 2 https://doi.org/10.1128/jb.156.2.828-836.1983	journal	January 1983
Adaptive evolution of Clostridium thermocellum ATCC 27405 on alternate carbon sources leads to altered fermentation profiles Daley, Steve R.; Kirby, Samantha; Sparling, Richard Canadian Journal of Microbiology, Vol. 70, Issue 9 https://doi.org/10.1139/cjm-2024-0004	journal	September 2024
Structure and Biogenesis of the cell Walls of Grasses Carpita, Nicholas C. Annual Review of Plant Physiology and Plant Molecular Biology, Vol. 47, Issue 1 https://doi.org/10.1146/annurev.arplant.47.1.445	journal	June 1996
Evaluation of the bioconversion of genetically modified switchgrass using simultaneous saccharification and fermentation and a consolidated bioprocessing approach Yee, Kelsey L.; Rodriguez Jr, Miguel; Tschaplinski, Timothy J. Biotechnology for Biofuels, Vol. 5, Issue 1 https://doi.org/10.1186/1754-6834-5-81	journal	January 2012
Consolidated bioprocessing of transgenic switchgrass by an engineered and evolved Clostridium thermocellum strain Yee, Kelsey L.; Rodriguez Jr, Miguel; Thompson, Olivia A. Biotechnology for Biofuels, Vol. 7, Issue 1 https://doi.org/10.1186/1754-6834-7-75	journal	January 2014
The exometabolome of Clostridium thermocellum reveals overflow metabolism at high cellulose loading Holwerda, Evert K.; Thorne, Philip G.; Olson, Daniel G. Biotechnology for Biofuels, Vol. 7, Issue 1 https://doi.org/10.1186/s13068-014-0155-1	journal	October 2014
Downregulation of GAUT12 in Populus deltoides by RNA silencing results in reduced recalcitrance, increased growth and reduced xylan and pectin in a woody biofuel feedstock Biswal, Ajaya K.; Hao, Zhangying; Pattathil, Sivakumar Biotechnology for Biofuels, Vol. 8, Issue 1 https://doi.org/10.1186/s13068-015-0218-y	journal	January 2015
Biological lignocellulose solubilization: comparative evaluation of biocatalysts and enhancement via cotreatment Paye, Julie M. D.; Guseva, Anna; Hammer, Sarah K. Biotechnology for Biofuels, Vol. 9, Issue 1 https://doi.org/10.1186/s13068-015-0412-y	journal	January 2016
Consolidated bioprocessing of Populus using Clostridium (Ruminiclostridium) thermocellum: a case study on the impact of lignin composition and structure Dumitrache, Alexandru; Akinosho, Hannah; Rodriguez, Miguel Biotechnology for Biofuels, Vol. 9, Issue 1 https://doi.org/10.1186/s13068-016-0445-x	journal	February 2016
Comparison of four glycosyl residue composition methods for effectiveness in detecting sugars from cell walls of dicot and grass tissues Biswal, Ajaya K.; Tan, Li; Atmodjo, Melani A. Biotechnology for Biofuels, Vol. 10, Issue 1 https://doi.org/10.1186/s13068-017-0866-1	journal	July 2017
The effect of switchgrass loadings on feedstock solubilization and biofuel production by Clostridium thermocellum Verbeke, Tobin J.; Garcia, Gabriela M.; Elkins, James G. Biotechnology for Biofuels, Vol. 10, Issue 1 https://doi.org/10.1186/s13068-017-0917-7	journal	November 2017
Comparative characterization of all cellulosomal cellulases from Clostridium thermocellum reveals high diversity in endoglucanase product formation essential for complex activity Leis, Benedikt; Held, Claudia; Bergkemper, Fabian Biotechnology for Biofuels, Vol. 10, Issue 1 https://doi.org/10.1186/s13068-017-0928-4	journal	October 2017
Defined tetra-allelic gene disruption of the 4-coumarate:coenzyme A ligase 1 (Pv4CL1) gene by CRISPR/Cas9 in switchgrass results in lignin reduction and improved sugar release Park, Jong-Jin; Yoo, Chang Geun; Flanagan, Amy Biotechnology for Biofuels, Vol. 10, Issue 1 https://doi.org/10.1186/s13068-017-0972-0	journal	November 2017
Designer biomass for next-generation biorefineries: leveraging recent insights into xylan structure and biosynthesis Smith, Peter J.; Wang, Hsin-Tzu; York, William S. Biotechnology for Biofuels, Vol. 10, Issue 1 https://doi.org/10.1186/s13068-017-0973-z	journal	November 2017
Development and use of a switchgrass (Panicum virgatum L.) transformation pipeline by the BioEnergy Science Center to evaluate plants for reduced cell wall recalcitrance Nelson, Richard S.; Stewart, C. Neal; Gou, Jiqing Biotechnology for Biofuels, Vol. 10, Issue 1 https://doi.org/10.1186/s13068-017-0991-x	journal	December 2017
Biomass augmentation through thermochemical pretreatments greatly enhances digestion of switchgrass by Clostridium thermocellum Kothari, Ninad; Holwerda, Evert K.; Cai, Charles M. Biotechnology for Biofuels, Vol. 11, Issue 1 https://doi.org/10.1186/s13068-018-1216-7	journal	August 2018
Composition and yield of non-cellulosic and cellulosic sugars in soluble and particulate fractions during consolidated bioprocessing of poplar biomass by Clostridium thermocellum Biswal, Ajaya K.; Hengge, Neal N.; Black, Ian M. Biotechnology for Biofuels and Bioproducts, Vol. 15, Issue 1 https://doi.org/10.1186/s13068-022-02119-9	journal	February 2022
Valorization of hemicellulose waste streams for moisture barrier coatings and hydrophobic films Josey, Daniel C.; Yadavalli, Nataraja S.; Moore, Jack C. Biotechnology for Sustainable Materials, Vol. 1, Issue 1 https://doi.org/10.1186/s44316-024-00009-8	journal	August 2024
Sequence of the Clostridium thermocellum Mannanase Gene man26B and Characterization of the Translated Product Kurokawa, Junji; Hemjinda, Eiakalak; Arai, Takamitsu Bioscience, Biotechnology, and Biochemistry, Vol. 65, Issue 3 https://doi.org/10.1271/bbb.65.548	journal	January 2001
Impact of Pretreated Switchgrass and Biomass Carbohydrates on Clostridium thermocellum ATCC 27405 Cellulosome Composition: A Quantitative Proteomic Analysis Raman, Babu; Pan, Chongle; Hurst, Gregory B. PLoS ONE, Vol. 4, Issue 4, Article No. e5271 https://doi.org/10.1371/journal.pone.0005271	journal	April 2009
Role of Pectinolytic Enzymes Identified in Clostridium thermocellum Cellulosome Chakraborty, Soumyadeep; Fernandes, Vania O.; Dias, Fernando M. V. PLOS ONE, Vol. 10, Issue 2 https://doi.org/10.1371/journal.pone.0116787	journal	February 2015
2023 Billion-Ton Report Langholtz, Matthew H. https://doi.org/10.23720/BT2023/2316165	report	January 2024
The emergence of Clostridium thermocellum as a high utility candidate for consolidated bioprocessing applications Akinosho, Hannah; Yee, Kelsey; Close, Dan Frontiers in Chemistry, Vol. 2 https://doi.org/10.3389/fchem.2014.00066	journal	August 2014
A genomic analysis reveals the diversity of cellulosome displaying bacteria Minor, Christine M.; Takayesu, Allen; Ha, Sung Min Frontiers in Microbiology, Vol. 15 https://doi.org/10.3389/fmicb.2024.1473396	journal	October 2024
Loss of Arabidopsis GAUT12/IRX8 causes anther indehiscence and leads to reduced G lignin associated with altered matrix polysaccharide deposition Hao, Zhangying; Avci, Utku; Tan, Li Frontiers in Plant Science, Vol. 5 https://doi.org/10.3389/fpls.2014.00357	journal	July 2014
Functional Analysis of Cellulose Synthase CesA4 and CesA6 Genes in Switchgrass (Panicum virgatum) by Overexpression and RNAi-Mediated Gene Silencing Mazarei, Mitra; Baxter, Holly L.; Li, Mi Frontiers in Plant Science, Vol. 9 https://doi.org/10.3389/fpls.2018.01114	journal	August 2018
Unraveling cell wall polysaccharides during blueberry ripening: insights into the roles of rhamnogalacturonan-I and arabinogalactan proteins in fruit firmness Sanhueza, Dayan; Balic-Norambuena, Iván; Sepúlveda-Orellana, Pablo Frontiers in Plant Science, Vol. 15 https://doi.org/10.3389/fpls.2024.1422917	journal	September 2024
Chemical Production Based on Biomass—Potential and Limits Kircher, Manfred Biomass, Vol. 5, Issue 1 https://doi.org/10.3390/biomass5010008	journal	February 2025
Clostridium thermocellum as a Promising Source of Genetic Material for Designer Cellulosomes: An Overview Ha-Tran, Dung Minh; Nguyen, Trinh Thi My; Huang, Chieh-Chen Catalysts, Vol. 11, Issue 8 https://doi.org/10.3390/catal11080996	journal	August 2021
Review of Lignocellulosic Biomass Pretreatment Using Physical, Thermal and Chemical Methods for Higher Yields in Bioethanol Production Woźniak, Adrian; Kuligowski, Ksawery; Świerczek, Lesław Sustainability, Vol. 17, Issue 1 https://doi.org/10.3390/su17010287	journal	January 2025

Similar Records

Composition and yield of non-cellulosic and cellulosic sugars in soluble and particulate fractions during consolidated bioprocessing of poplar biomass by Clostridium thermocellum

Journal Article · Mon Feb 28 00:00:00 UTC 2022 · Biotechnology for Biofuels and Bioproducts · OSTI ID:1846685

Biswal, Ajaya K.; Hengge, Neal N.; Black, Ian M.; +7 more

Biomass augmentation through thermochemical pretreatments greatly enhances digestion of switchgrass by Clostridium thermocellum

Journal Article · Sat Aug 04 00:00:00 UTC 2018 · Biotechnology for Biofuels · OSTI ID:1626995

Kothari, Ninad; Holwerda, Evert K.; Cai, Charles M.; +2 more

Comparative analysis of the ability of Clostridium clariflavum strains and Clostridium thermocellumto utilize hemicellulose and unpretreated plant material

Journal Article · Tue Nov 18 00:00:00 UTC 2014 · Biotechnology for Biofuels · OSTI ID:1259857

Izquierdo, Javier A.; Pattathil, Sivakumar; Guseva, Anna; +2 more

Related Subjects

09 BIOMASS FUELS
consolidated bioprocessing
lignocellulosic feedstocks
liquid fuels

Title: Rhamnogalacturonan I is a recalcitrant pectin domain during Clostridium thermocellum-mediated deconstruction of switchgrass biomass

Citation Formats

References (80)

Similar Records

Related Subjects