Revealing structure and shaping priorities in plant and fungal cell wall architecture via solid-state NMR

Xiao, Peng; Sahu, Priya; Pfaff, Sarah A.; Ankur, Ankur; Ranasinghe, Yohara K.; Gow, Neil A. R.; Latgé, Jean-Paul; Cosgrove, Daniel J.; Wang, Tuo

doi:10.1016/j.tcsw.2025.100159

Title: Revealing structure and shaping priorities in plant and fungal cell wall architecture via solid-state NMR

Journal Article · Fri Oct 31 00:00:00 UTC 2025 · The Cell Surface

DOI: https://doi.org/10.1016/j.tcsw.2025.100159 · OSTI ID:3003749

Xiao, Peng ^[1]; Sahu, Priya ^[1]; Pfaff, Sarah A. ^[2]; Ankur, Ankur ^[1]; Ranasinghe, Yohara K. ^[1]; Gow, Neil A. R. ^[3]; Latgé, Jean-Paul ^[4]; Cosgrove, Daniel J. ^[2]; Wang, Tuo ^[1]

Michigan State Univ., East Lansing, MI (United States)
Pennsylvania State Univ., University Park, PA (United States)
Univ. of Exeter, Devon (United Kingdom)
Pasteur Inst. (France)

Plant and fungal cell walls are essential for growth, adaptation, and survival, with their intricate architectures dictating both resistance to stress and susceptibility to antifungal or biomass-degrading strategies. Understanding how these walls are built, remodeled, and function at the molecular level is therefore central to both clinical and biotechnological applications. Solid-state nuclear magnetic resonance (ssNMR) has emerged as a uniquely powerful tool for this purpose, as it reveals the structure, dynamics, and interactions of intact biopolymers without disrupting their native organization. Using this approach, recent studies have shown how structural polymorphism, polymer-polymer interactions, and species-specific remodeling govern mechanical integrity, drug resistance, and stress adaptation. Applications highlighted here include lignin-carbohydrate packing during plant stem maturation, fungal wall reorganization under treatment by wall-targeting antifungals such as echinocandin and nikkomycin, and the functional diversity of glucans, chitins, and mannans. Together, these insights uncover conserved principles of polymer assembly across kingdoms while informing new opportunities for antifungal development and biomass utilization. Ongoing advances in sensitivity and resolution are expected to broaden the reach of ssNMR and further accelerate its role in linking structural heterogeneity to biosynthetic complexity and biological function.

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Research Organization:: Michigan State Univ., East Lansing, MI (United States)

Sponsoring Organization:: USDOE

Grant/Contract Number:: SC0001090; SC0023702

OSTI ID:: 3003749

Journal Information:: The Cell Surface, Journal Name: The Cell Surface Vol. 14; ISSN 2468-2330

Publisher:: ElsevierCopyright Statement

Country of Publication:: United States

Language:: English

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