Hydrophobins from Aspergillus Mediate Fungal Interactions with Microplastics

Klauer, Ross R.; Silvestri, Rachel; White, Hanna; Das, Milton; Hayes, Richard D.; Riley, Robert; Lipzen, Anna; Barry, Kerrie; Grigoriev, Igor V.; Talag, Jayson; Bunting, Victoria Mae; Stevenson, Zachary; Demokritou, Philip; Solomon, Kevin V.; Blenner, Mark

doi:10.1021/acs.est.5c01771

Title: Hydrophobins from Aspergillus Mediate Fungal Interactions with Microplastics

Journal Article · Wed Jul 09 00:00:00 UTC 2025 · Environmental Science and Technology

DOI: https://doi.org/10.1021/acs.est.5c01771 · OSTI ID:2999542

Klauer, Ross R. ^[1]; Silvestri, Rachel ^[1]; White, Hanna ^[1]; Das, Milton ^[2]; Hayes, Richard D. ^[3]; Riley, Robert ^[3]; Lipzen, Anna ^[3]; Barry, Kerrie ^[3]; Grigoriev, Igor V. ^[4]; Talag, Jayson ^[5]; Bunting, Victoria Mae ^[5]; Stevenson, Zachary ^[1];

^[2];

^[1];

^[1]

University of Delaware, Newark, DE (United States)
Rutgers University, Piscataway, NJ (United States)
Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)
Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States); University of California, Berkeley, CA (United States)
Arizona Genomics Institute, Tucson, AZ (United States)

Microplastics cause negative environmental consequences such as the release of toxic additive leachates, increased greenhouse gas emissions during degradation, and threaten food chains . Microplastic particles are known to serve as a vector for transport of microbes (fungi and bacteria) to new environments, threatening biodiversity. Robust biofilm formation makes fungi a candidate to collect and remediate environmental microplastics. However, fungal-microplastic colonization mechanisms have yet to be explored. In this work, we aim to understand which fungal molecules mediate microplastics binding. We examine common fungal genus Aspergillus , which we found binds microplastics tightly, removing particles from suspension. Upon inoculation of Aspergilli with microplastics particles, up to 3.85 ± 1.48 g of microplastics were flocculated per gram of dry fungal biomass; this phenomenon was observed across various plastics ranging in size from 0.05 to 5 mm. Gene knockouts revealed that hydrophobins drive microplastic-fungi binding, evidenced by a decrease in flocculation relative to wild-type Aspergillus fumigatus. Moreover, purified hydrophobins flocculated microplastics independently of the fungus, validating their ability to bind to microplastics. Furthermore, our work elucidates a role for hydrophobins in fungal colonization of microplastics and highlights a target for mitigating the harm of microplastics through engineered fungal-microplastic interactions.

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Research Organization:: Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States); Univ. of Delaware, Newark, DE (United States)

Sponsoring Organization:: NIFA/USDA; NIH/NIEHS; National Institutes of Health (NIH); USDOE Office of Science (SC), Basic Energy Sciences (BES). Scientific User Facilities (SUF); USDOE Office of Science (SC), Biological and Environmental Research (BER). Biological Systems Science (BSS)

Grant/Contract Number:: AC02-05CH11231; AC05-76RL01830; SC0022018; SC0023085

OSTI ID:: 2999542

Journal Information:: Environmental Science and Technology, Journal Name: Environmental Science and Technology Journal Issue: 28 Vol. 59; ISSN 1520-5851; ISSN 0013-936X

Publisher:: American Chemical Society (ACS)Copyright Statement

Country of Publication:: United States

Language:: English

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