Identification and overexpression of endogenous transcription factors to enhance lipid accumulation in the biotechnologically relevant species Chlamydomonas pacifica

Gupta, Abhishek; Oliver, Aaron; Molino, João Vitor Dutra; Wnuk-Fink, Kathryn M. J.; Tessman, Marissa; Kang, Kalisa; do Espirito Santo, Évellin; Torres-Tiji, Yasin; Burkart, Michael D.; Mayfield, Stephen

doi:10.1016/j.algal.2025.104192

Title: Identification and overexpression of endogenous transcription factors to enhance lipid accumulation in the biotechnologically relevant species Chlamydomonas pacifica

Journal Article · Fri Jul 04 00:00:00 UTC 2025 · Algal Research

DOI: https://doi.org/10.1016/j.algal.2025.104192 · OSTI ID:2583161

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University of California San Diego, La Jolla, CA (United States); J. Craig Venter Institute, La Jolla, CA (United States)
University of California San Diego, La Jolla, CA (United States)
Algenesis Inc., Cardiff, CA (United States)
University of California San Diego, La Jolla, CA (United States); University of São Paulo (Brazil)
University of California San Diego, La Jolla, CA (United States); Algenesis Inc., Cardiff, CA (United States)

Sustainable low-carbon energy solutions are critical to mitigating global carbon emissions. Algae-based platforms offer potential by converting carbon dioxide into valuable products while aiding carbon sequestration. However, scaling algae cultivation faces challenges like contamination in outdoor systems. Previously, our lab evolved Chlamydomonas pacifica, an extremophile green alga, which tolerates high temperature, pH, salinity, and light, making it ideal for large-scale bioproduct production, including biodiesel. Here, we enhanced lipid accumulation in evolved C. pacifica by identifying and overexpressing key endogenous transcription factors through genome-wide in-silico analysis and in-vivo testing. These factors include Lipid Remodeling Regulator 1 (CpaLRL1), Nitrogen Response Regulator 1 (CpaNRR1), Compromised Hydrolysis of Triacylglycerols 7 (CpaCHT7), and Phosphorus Starvation Response 1 (CpaPSR1). Under nitrogen deprivation, CpaLRL1, CpaNRR1, and CpaCHT7 overexpression enhanced lipid accumulation compared to wild-type. However, CpaPSR1 increased lipid accumulation compared to wild-type in normal media and did not increase further under nitrogen deprivation, highlighting the difference in function based on media conditions. Notably, lipid analysis of CpaPSR1 under normal media conditions revealed a 2.4-fold increase in triglycerides (TAGs) compared to the wild-type, highlighting its potential for biodiesel production. This approach provides a framework for transcription factor-focused metabolic engineering in algae, advancing bioenergy and biomaterial production.

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Research Organization:: University of California, San Diego, La Jolla, CA (United States)

Sponsoring Organization:: National Institute of Neurological Disorders and Stroke (NINDS); USDOE Office of Energy Efficiency and Renewable Energy (EERE)

Grant/Contract Number:: EE0009671

OSTI ID:: 2583161

Journal Information:: Algal Research, Journal Name: Algal Research Vol. 90; ISSN 2211-9264

Publisher:: ElsevierCopyright Statement

Country of Publication:: United States

Language:: English

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Journal Article · Wed Jun 19 00:00:00 UTC 2024 · Biotechnology for Biofuels and Bioproducts · OSTI ID:2375538

Anthony, Winston E.; Geng, Weitao; Diao, Jinjin; +6 more

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Transcription factors

Title: Identification and overexpression of endogenous transcription factors to enhance lipid accumulation in the biotechnologically relevant species Chlamydomonas pacifica

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