A distinct class of ferredoxin:NADP+ oxidoreductase enzymes driving thermophilic ethanol production

Huang, Shu; Saad Imran, Syed Muhammad; Lanahan, Anthony A.; Hammer, Sarah K.; Lubner, Carolyn E.; Lynd, Lee R.; Olson, Daniel G.

doi:10.1016/j.jbc.2025.110263

Title: A distinct class of ferredoxin:NADP⁺ oxidoreductase enzymes driving thermophilic ethanol production

Journal Article · Wed May 21 00:00:00 UTC 2025 · Journal of Biological Chemistry

DOI: https://doi.org/10.1016/j.jbc.2025.110263 · OSTI ID:2574685

^[1];

^[2]; Lanahan, Anthony A. ^[1]; Hammer, Sarah K. ^[3];

^[2]; Lynd, Lee R. ^[1];

^[1]

Dartmouth College, Hanover, NH (United States); Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States). Center for Bioenergy Innovation (CBI)
National Renewable Energy Laboratory (NREL), Golden, CO (United States)
Dartmouth College, Hanover, NH (United States)

Biofuel production from lignocellulosic biomass offers a transformative solution to reduce global fossil fuel dependency. Certain thermophilic anaerobes, including Clostridium thermocellum, show promise for renewable ethanol production due to their ability to break down plant material at high temperatures. However, achieving commercially viable ethanol yields has proven challenging despite extensive engineering efforts. Here, we characterized 27 ferredoxin:NADP⁺ oxidoreductase (Fnor) enzymes for their enzyme activity, nicotinamide cofactor specificity, thermotolerance, and functional expression in C. thermocellum. We identified a subset of 10 of these enzymes as a novel class of Fnor enzymes suited for metabolic pathways aimed at high-titer ethanol production. When expressed in engineered C. thermocellum, these enzymes increased ethanol production up to 2.2-fold. These findings establish a novel ethanol pathway and provide insights into physiological roles and biotechnological applications of this new class of Fnor enzymes.

View Accepted Manuscript (DOE)

Cite

Export

Save

Research Organization:: National Renewable Energy Laboratory (NREL), Golden, CO (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Basic Energy Sciences (BES). Scientific User Facilities (SUF)

Grant/Contract Number:: AC02-05CH11231; AC36-08GO28308

OSTI ID:: 2574685

Report Number(s):: NREL/JA--2700-93966

Journal Information:: Journal of Biological Chemistry, Journal Name: Journal of Biological Chemistry Journal Issue: 7 Vol. 301; ISSN 0021-9258

Publisher:: American Society for Biochemistry and Molecular BiologyCopyright Statement

Country of Publication:: United States

Language:: English

References (92)

The need for biofuels as part of a low carbon energy future Fulton, Lewis M.; Lynd, Lee R.; Körner, Alexander Biofuels, Bioproducts and Biorefining, Vol. 9, Issue 5 https://doi.org/10.1002/bbb.1559	journal	June 2015
Crystal structure analysis of Bacillus subtilis ferredoxin-NADP+ oxidoreductase and the structural basis for its substrate selectivity Komori, Hirofumi; Seo, Daisuke; Sakurai, Takeshi Protein Science, Vol. 19, Issue 12 https://doi.org/10.1002/pro.508	journal	November 2010
Refined Crystal Structure of Spinach Ferredoxin Reductase at 1.7 Å Resolution: Oxidized, Reduced and 2′-Phospho-5′-AMP Bound States Bruns, Christopher M.; Karplus, Andrew P. Journal of Molecular Biology, Vol. 247, Issue 1 https://doi.org/10.1006/jmbi.1994.0127	journal	March 1995
Identification of a new class of nitrogen fixation genes in Rhodobacter capsalatus: a putative membrane complex involved in electron transport to nitrogenase Schmehl, Manfred; Jahn, Andreas; Meyer zu Vilsendorf, Andreas MGG Molecular & General Genetics, Vol. 241-241, Issue 5-6 https://doi.org/10.1007/BF00279903	journal	December 1993
Novel structure and redox chemistry of the prosthetic groups of the iron-sulfur flavoprotein sulfide dehydrogenase from Pyrococcus furiosus; evidence for a [2Fe-2S] cluster with Asp(Cys)3 ligands Hagen, W. R.; Silva, P. J.; Amorim, M. A. JBIC Journal of Biological Inorganic Chemistry, Vol. 5, Issue 4 https://doi.org/10.1007/PL00021452	journal	August 2000
Biochemistry, evolution and physiological function of the Rnf complex, a novel ion-motive electron transport complex in prokaryotes Biegel, Eva; Schmidt, Silke; González, José M. Cellular and Molecular Life Sciences, Vol. 68, Issue 4 https://doi.org/10.1007/s00018-010-0555-8	journal	November 2010
Mutant selection and phenotypic and genetic characterization of ethanol-tolerant strains of Clostridium thermocellum Shao, Xiongjun; Raman, Babu; Zhu, Mingjun Applied Microbiology and Biotechnology, Vol. 92, Issue 3 https://doi.org/10.1007/s00253-011-3492-z	journal	August 2011
Interaction and electron transfer between ferredoxin–NADP+ oxidoreductase and its partners: structural, functional, and physiological implications Mulo, Paula; Medina, Milagros Photosynthesis Research, Vol. 134, Issue 3 https://doi.org/10.1007/s11120-017-0372-0	journal	March 2017
The reduction of iodonitrotetrazolium chloride by ferredoxin-NADP+ reductase: A new tool for the characterization of the spinach chloroplast flavoprotein Zanetti, Giuliana Plant Science Letters, Vol. 23, Issue 1 https://doi.org/10.1016/0304-4211(81)90025-0	journal	October 1981
Transformation of Clostridium Thermocellum by Electroporation Olson, Daniel G.; Lynd, Lee R. Cellulases https://doi.org/10.1016/B978-0-12-415931-0.00017-3	book	January 2012
Mechanism of Coenzyme Recognition and Binding Revealed by Crystal Structure Analysis of Ferredoxin–NADP+ Reductase Complexed with NADP+ Hermoso, Juan A.; Mayoral, Tomas; Faro, Merche Journal of Molecular Biology, Vol. 319, Issue 5 https://doi.org/10.1016/S0022-2836(02)00388-1	journal	June 2002
Basic local alignment search tool Altschul, Stephen F.; Gish, Warren; Miller, Webb Journal of Molecular Biology, Vol. 215, Issue 3, p. 403-410 https://doi.org/10.1016/S0022-2836(05)80360-2	journal	October 1990
Structure of Dihydroorotate Dehydrogenase B Rowland, Paul; Nørager, Sofie; Jensen, Kaj Frank Structure, Vol. 8, Issue 12 https://doi.org/10.1016/S0969-2126(00)00530-X	journal	December 2000
Clostridium thermocellum: A microbial platform for high-value chemical production from lignocellulose Mazzoli, R.; Olson, D. G. Advances in Applied Microbiology, p. 111-161 https://doi.org/10.1016/bs.aambs.2020.07.004	book	August 2020
Structural and functional diversity of ferredoxin-NADP+ reductases Aliverti, Alessandro; Pandini, Vittorio; Pennati, Andrea Archives of Biochemistry and Biophysics, Vol. 474, Issue 2 https://doi.org/10.1016/j.abb.2008.02.014	journal	June 2008
Overview of structurally homologous flavoprotein oxidoreductases containing the low Mr thioredoxin reductase-like fold – A functionally diverse group Hammerstad, Marta; Hersleth, Hans-Petter Archives of Biochemistry and Biophysics, Vol. 702 https://doi.org/10.1016/j.abb.2021.108826	journal	May 2021
Energy conservation via electron bifurcating ferredoxin reduction and proton/Na+ translocating ferredoxin oxidation Buckel, Wolfgang; Thauer, Rudolf K. Biochimica et Biophysica Acta (BBA) - Bioenergetics, Vol. 1827, Issue 2 https://doi.org/10.1016/j.bbabio.2012.07.002	journal	February 2013
Roles of the F-domain in [FeFe] hydrogenase Gauquelin, Charles; Baffert, Carole; Richaud, Pierre Biochimica et Biophysica Acta (BBA) - Bioenergetics, Vol. 1859, Issue 2 https://doi.org/10.1016/j.bbabio.2017.08.010	journal	February 2018
Molecular mechanism of metabolic NAD(P)H-dependent electron-transfer systems: The role of redox cofactors Iyanagi, Takashi Biochimica et Biophysica Acta (BBA) - Bioenergetics, Vol. 1860, Issue 3 https://doi.org/10.1016/j.bbabio.2018.11.014	journal	March 2019
Review of NAD(P)H-dependent oxidoreductases: Properties, engineering and application Sellés Vidal, Lara; Kelly, Ciarán L.; Mordaka, Paweł M. Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics, Vol. 1866, Issue 2 https://doi.org/10.1016/j.bbapap.2017.11.005	journal	February 2018
Closing the carbon balance for fermentation by Clostridium thermocellum (ATCC 27405) Ellis, Lucas D.; Holwerda, Evert K.; Hogsett, David Bioresource Technology, Vol. 103, Issue 1 https://doi.org/10.1016/j.biortech.2011.09.128	journal	January 2012
Ethanol production by engineered thermophiles Olson, Daniel G.; Sparling, Richard; Lynd, Lee R. Current Opinion in Biotechnology, Vol. 33 https://doi.org/10.1016/j.copbio.2015.02.006	journal	June 2015
End-product pathways in the xylose fermenting bacterium, Thermoanaerobacterium saccharolyticum Joe Shaw, A.; Jenney, Francis E.; Adams, Michael W. W. Enzyme and Microbial Technology, Vol. 42, Issue 6 https://doi.org/10.1016/j.enzmictec.2008.01.005	journal	May 2008
The role of AdhE on ethanol tolerance and production in Clostridium thermocellum Pech-Canul, Angel; Hammer, Sarah K.; Ziegler, Samantha J. Journal of Biological Chemistry, Vol. 300, Issue 8 https://doi.org/10.1016/j.jbc.2024.107559	journal	August 2024
Expression and characterization of monofunctional alcohol dehydrogenase enzymes in Clostridium thermocellum Chiarelli, Daniela Prates; Sharma, Bishal Dev; Hon, Shuen Metabolic Engineering Communications, Vol. 19 https://doi.org/10.1016/j.mec.2024.e00243	journal	December 2024
Engineering electron metabolism to increase ethanol production in Clostridium thermocellum Lo, Jonathan; Olson, Daniel G.; Murphy, Sean Jean-Loup Metabolic Engineering, Vol. 39 https://doi.org/10.1016/j.ymben.2016.10.018	journal	January 2017
The ethanol pathway from Thermoanaerobacterium saccharolyticum improves ethanol production in Clostridium thermocellum Hon, Shuen; Olson, Daniel G.; Holwerda, Evert K. Metabolic Engineering, Vol. 42 https://doi.org/10.1016/j.ymben.2017.06.011	journal	July 2017
Characterization of the Clostridium thermocellum AdhE, NfnAB, ferredoxin and Pfor proteins for their ability to support high titer ethanol production in Thermoanaerobacterium saccharolyticum Cui, Jingxuan; Olson, Daniel G.; Lynd, Lee R. Metabolic Engineering, Vol. 51 https://doi.org/10.1016/j.ymben.2018.09.006	journal	January 2019
Thermodynamic analysis of the pathway for ethanol production from cellobiose in Clostridium thermocellum Dash, Satyakam; Olson, Daniel G.; Joshua Chan, Siu Hung Metabolic Engineering, Vol. 55 https://doi.org/10.1016/j.ymben.2019.06.006	journal	September 2019
Impact of an N-terminal Polyhistidine Tag on Protein Thermal Stability Booth, William T.; Schlachter, Caleb R.; Pote, Swanandi ACS Omega, Vol. 3, Issue 1 https://doi.org/10.1021/acsomega.7b01598	journal	January 2018
Streamlining the Design-to-Build Transition with Build-Optimization Software Tools Oberortner, Ernst; Cheng, Jan-Fang; Hillson, Nathan J. ACS Synthetic Biology, Vol. 6, Issue 3 https://doi.org/10.1021/acssynbio.6b00200	journal	November 2016
A Covalent Modification of NADP⁺ Revealed by the Atomic Resolution Structure of FprA, a Mycobacterium tuberculosis Oxidoreductase^, Bossi, Roberto T.; Aliverti, Alessandro; Raimondi, Debora Biochemistry, Vol. 41, Issue 28 https://doi.org/10.1021/bi025858a	journal	June 2002
Thermodynamic Basis of Electron Transfer in Dihydroorotate Dehydrogenase B from Lactococcus lactis : Analysis by Potentiometry, EPR Spectroscopy, and ENDOR Spectroscopy ^† Mohsen, Al-Walid A.; Rigby, Stephen E. J.; Jensen, Kaj Frank Biochemistry, Vol. 43, Issue 21 https://doi.org/10.1021/bi036179i	journal	June 2004
Mechanistic insights into energy conservation by flavin-based electron bifurcation Lubner, Carolyn E.; Jennings, David P.; Mulder, David W. Nature Chemical Biology, Vol. 13, Issue 6 https://doi.org/10.1038/nchembio.2348	journal	April 2017
Autotrophy at the thermodynamic limit of life: a model for energy conservation in acetogenic bacteria Schuchmann, Kai; Müller, Volker Nature Reviews Microbiology, Vol. 12, Issue 12 https://doi.org/10.1038/nrmicro3365	journal	November 2014
The semiquinone swing in the bifurcating electron transferring flavoprotein/butyryl-CoA dehydrogenase complex from Clostridium difficile Demmer, Julius K.; Pal Chowdhury, Nilanjan; Selmer, Thorsten Nature Communications, Vol. 8, Issue 1 https://doi.org/10.1038/s41467-017-01746-3	journal	November 2017
Molecular characterization of the missing electron pathways for butanol synthesis in Clostridium acetobutylicum Foulquier, Céline; Rivière, Antoine; Heulot, Mathieu Nature Communications, Vol. 13, Issue 1 https://doi.org/10.1038/s41467-022-32269-1	journal	August 2022
Purification and structural characterization of the Na+-translocating ferredoxin: NAD+ reductase (Rnf) complex of Clostridium tetanomorphum Vitt, Stella; Prinz, Simone; Eisinger, Martin Nature Communications, Vol. 13, Issue 1 https://doi.org/10.1038/s41467-022-34007-z	journal	October 2022
Molecular architecture and electron transfer pathway of the Stn family transhydrogenase Kumar, Anuj; Kremp, Florian; Roth, Jennifer Nature Communications, Vol. 14, Issue 1 https://doi.org/10.1038/s41467-023-41212-x	journal	September 2023
Architecture of the RNF1 complex that drives biological nitrogen fixation Zhang, Lin; Einsle, Oliver Nature Chemical Biology, Vol. 20, Issue 8 https://doi.org/10.1038/s41589-024-01641-1	journal	June 2024
The Rnf complex is a Na+ coupled respiratory enzyme in a fermenting bacterium, Thermotoga maritima Kuhns, Martin; Trifunović, Dragan; Huber, Harald Communications Biology, Vol. 3, Issue 1 https://doi.org/10.1038/s42003-020-01158-y	journal	August 2020
Toward low-cost biological and hybrid biological/catalytic conversion of cellulosic biomass to fuels Lynd, Lee R.; Beckham, Gregg T.; Guss, Adam M. Energy & Environmental Science, Vol. 15, Issue 3 https://doi.org/10.1039/D1EE02540F	journal	January 2022
The genome of Syntrophus aciditrophicus: Life at the thermodynamic limit of microbial growth McInerney, M. J.; Rohlin, L.; Mouttaki, H. Proceedings of the National Academy of Sciences, Vol. 104, Issue 18 https://doi.org/10.1073/pnas.0610456104	journal	April 2007
Metabolic engineering of a thermophilic bacterium to produce ethanol at high yield Shaw, A. J.; Podkaminer, K. K.; Desai, S. G. Proceedings of the National Academy of Sciences, Vol. 105, Issue 37, p. 13769-13774 https://doi.org/10.1073/pnas.0801266105	journal	September 2008
Mutant alcohol dehydrogenase leads to improved ethanol tolerance in Clostridium thermocellum Brown, S. D.; Guss, A. M.; Karpinets, T. V. Proceedings of the National Academy of Sciences, Vol. 108, Issue 33 https://doi.org/10.1073/pnas.1102444108	journal	August 2011
An uncharacteristically low-potential flavin governs the energy landscape of electron bifurcation Wise, Courtney E.; Ledinina, Anastasia E.; Mulder, David W. Proceedings of the National Academy of Sciences, Vol. 119, Issue 12 https://doi.org/10.1073/pnas.2117882119	journal	March 2022
Crystal Structure of the Productive Ternary Complex of Dihydropyrimidine Dehydrogenase with NADPH and 5-Iodouracil Dobritzsch, Doreen; Ricagno, Stefano; Schneider, Gunter Journal of Biological Chemistry, Vol. 277, Issue 15 https://doi.org/10.1074/jbc.M111877200	journal	April 2002
Insights into Flavin-based Electron Bifurcation via the NADH-dependent Reduced Ferredoxin:NADP Oxidoreductase Structure Demmer, Julius K.; Huang, Haiyan; Wang, Shuning Journal of Biological Chemistry, Vol. 290, Issue 36 https://doi.org/10.1074/jbc.M115.656520	journal	July 2015
Two functionally distinct NADP ⁺ -dependent ferredoxin oxidoreductases maintain the primary redox balance of Pyrococcus furiosus Nguyen, Diep M. N.; Schut, Gerrit J.; Zadvornyy, Oleg A. Journal of Biological Chemistry, Vol. 292, Issue 35 https://doi.org/10.1074/jbc.M117.794172	journal	July 2017
The flexible N-terminus of BchL autoinhibits activity through interaction with its [4Fe-4S] cluster and released upon ATP binding Corless, Elliot I.; Saad Imran, Syed Muhammad; Watkins, Maxwell B. Journal of Biological Chemistry, Vol. 296 https://doi.org/10.1074/jbc.RA120.016278	journal	January 2021
COBALT: constraint-based alignment tool for multiple protein sequences Papadopoulos, J. S.; Agarwala, R. Bioinformatics, Vol. 23, Issue 9 https://doi.org/10.1093/bioinformatics/btm076	journal	March 2007
An integrated open framework for thermodynamics of reactions that combines accuracy and coverage Noor, Elad; Bar-Even, Arren; Flamholz, Avi Bioinformatics, Vol. 28, Issue 15 https://doi.org/10.1093/bioinformatics/bts317	journal	May 2012
eQuilibrator 3.0: a database solution for thermodynamic constant estimation Beber, Moritz E.; Gollub, Mattia G.; Mozaffari, Dana Nucleic Acids Research, Vol. 50, Issue D1 https://doi.org/10.1093/nar/gkab1106	journal	November 2021
The conserved domain database in 2023 Wang, Jiyao; Chitsaz, Farideh; Derbyshire, Myra K. Nucleic Acids Research, Vol. 51, Issue D1 https://doi.org/10.1093/nar/gkac1096	journal	December 2022
InterPro in 2022 Paysan-Lafosse, Typhaine; Blum, Matthias; Chuguransky, Sara Nucleic Acids Research, Vol. 51, Issue D1 https://doi.org/10.1093/nar/gkac993	journal	November 2022
IMG: the integrated microbial genomes database and comparative analysis system Markowitz, V. M.; Chen, I. -M. A.; Palaniappan, K. Nucleic Acids Research, Vol. 40, Issue D1 https://doi.org/10.1093/nar/gkr1044	journal	December 2011
The Pfam protein families database: towards a more sustainable future Finn, Robert D.; Coggill, Penelope; Eberhardt, Ruth Y. Nucleic Acids Research, Vol. 44, Issue D1 https://doi.org/10.1093/nar/gkv1344	journal	December 2015
Functional Assignment of the ORF2-iscS-iscU-iscA-hscB-hscA-fdx-0RF3 Gene Cluster Involved in the Assembly of Fe-S Clusters in Escherichia coli Takahashi, Y.; Nakamura, M. Journal of Biochemistry, Vol. 126, Issue 5 https://doi.org/10.1093/oxfordjournals.jbchem.a022535	journal	November 1999
The Complete Purification and Characterization of Three Forms of Ferredoxin-NADP+ Oxidoreductase from a Thermophilic Cyanobacterium Synechococcuselongatus Nakajima, Masato; Sakamoto, Toshio; Wada, Keishiro Plant and Cell Physiology, Vol. 43, Issue 5 https://doi.org/10.1093/pcp/pcf058	journal	May 2002
WebLogo: A Sequence Logo Generator Crooks, Gavin E.; Hon, Gary; Chandonia, John-Marc Genome Research, Vol. 14, Issue 6, p. 1188-1190 https://doi.org/10.1101/gr.849004	journal	May 2004
Cloning, expression, crystallization and preliminary X-ray studies of the ferredoxin–NAD(P)⁺reductase from the thermophilic cyanobacteriumThermosynechococcus elongatusBP-1 Liauw, Pasqual; Mashiba, Tomohiro; Kopczak, Marta Acta Crystallographica Section F Structural Biology and Crystallization Communications, Vol. 68, Issue 9 https://doi.org/10.1107/S1744309112031910	journal	August 2012
The future of bioenergy Reid, Walter V.; Ali, Mariam K.; Field, Christopher B. Global Change Biology, Vol. 26, Issue 1 https://doi.org/10.1111/gcb.14883	journal	December 2019
Ferredoxin-NADP⁺reductase from the thermophilic hydrogen-oxidizing bacterium,Hydrogenobacter thermophilusâTK-6 Ikeda, Takeshi; Nakamura, Miyuki; Arai, Hiroyuki FEMS Microbiology Letters, Vol. 297, Issue 1 https://doi.org/10.1111/j.1574-6968.2009.01667.x	journal	August 2009
Life on the thermodynamic edge: Respiratory growth of an acetotrophic methanogen Prakash, Divya; Chauhan, Shikha S.; Ferry, James G. Science Advances, Vol. 5, Issue 8 https://doi.org/10.1126/sciadv.aaw9059	journal	August 2019
Ferredoxin:NAD ⁺ Oxidoreductase of Thermoanaerobacterium saccharolyticum and Its Role in Ethanol Formation Tian, Liang; Lo, Jonathan; Shao, Xiongjun Applied and Environmental Microbiology, Vol. 82, Issue 24 https://doi.org/10.1128/AEM.02130-16	journal	September 2016
Deletion of nfnAB in Thermoanaerobacterium saccharolyticum and Its Effect on Metabolism Lo, Jonathan; Zheng, Tianyong; Olson, Daniel G. Journal of Bacteriology, Vol. 197, Issue 18 https://doi.org/10.1128/JB.00347-15	journal	June 2015
Electron Bifurcation Involved in the Energy Metabolism of the Acetogenic Bacterium Moorella thermoacetica Growing on Glucose or H2 plus CO2 Huang, H.; Wang, S.; Moll, J. Journal of Bacteriology, Vol. 194, Issue 14 https://doi.org/10.1128/JB.00385-12	journal	May 2012
NADP+ Reduction with Reduced Ferredoxin and NADP+ Reduction with NADH Are Coupled via an Electron-Bifurcating Enzyme Complex in Clostridium kluyveri Wang, S.; Huang, H.; Moll, J. Journal of Bacteriology, Vol. 192, Issue 19 https://doi.org/10.1128/JB.00612-10	journal	July 2010
Characterization of a Thioredoxin-Thioredoxin Reductase System from the Hyperthermophilic Bacterium Thermotoga maritima Yang, X.; Ma, K. Journal of Bacteriology, Vol. 192, Issue 5 https://doi.org/10.1128/JB.01035-09	journal	January 2010
Microbial Cellulose Utilization: Fundamentals and Biotechnology Lynd, L. R.; Weimer, P. J.; van Zyl, W. H. Microbiology and Molecular Biology Reviews, Vol. 66, Issue 3, p. 506-577 https://doi.org/10.1128/MMBR.66.3.506-577.2002	journal	September 2002
Cellulase, Clostridia, and Ethanol Demain, A. L.; Newcomb, M.; Wu, J. H. D. Microbiology and Molecular Biology Reviews, Vol. 69, Issue 1, p. 124-154 https://doi.org/10.1128/MMBR.69.1.124-154.2005	journal	March 2005
Increasing the Thermodynamic Driving Force of the Phosphofructokinase Reaction in Clostridium thermocellum Hon, Shuen; Jacobson, Tyler; Stevenson, David M. Applied and Environmental Microbiology, Vol. 88, Issue 22 https://doi.org/10.1128/aem.01258-22	journal	November 2022
Phycobilisomes Harbor FNR _L in Cyanobacteria Liu, Haijun; Weisz, Daniel A.; Zhang, Mengru M. mBio, Vol. 10, Issue 2 https://doi.org/10.1128/mBio.00669-19	journal	April 2019
Dcm methylation is detrimental to plasmid transformation in Clostridium thermocellum Guss, Adam M.; Olson, Daniel G.; Caiazza, Nicky C. Biotechnology for Biofuels, Vol. 5, Issue 1 https://doi.org/10.1186/1754-6834-5-30	journal	January 2012
Genome-scale resources for Thermoanaerobacterium saccharolyticum Currie, Devin H.; Raman, Babu; Gowen, Christopher M. BMC Systems Biology, Vol. 9, Issue 1 https://doi.org/10.1186/s12918-015-0159-x	journal	June 2015
Simultaneous achievement of high ethanol yield and titer in Clostridium thermocellum Tian, Liang; Papanek, Beth; Olson, Daniel G. Biotechnology for Biofuels, Vol. 9, Issue 1 https://doi.org/10.1186/s13068-016-0528-8	journal	June 2016
Strain and bioprocess improvement of a thermophilic anaerobe for the production of ethanol from wood Herring, Christopher D.; Kenealy, William R.; Joe Shaw, A. Biotechnology for Biofuels, Vol. 9, Issue 1 https://doi.org/10.1186/s13068-016-0536-8	journal	June 2016
Metabolome analysis reveals a role for glyceraldehyde 3-phosphate dehydrogenase in the inhibition of C. thermocellum by ethanol Tian, Liang; Perot, Skyler J.; Stevenson, David Biotechnology for Biofuels, Vol. 10, Issue 1 https://doi.org/10.1186/s13068-017-0961-3	journal	November 2017
Expressing the Thermoanaerobacterium saccharolyticum pforA in engineered Clostridium thermocellum improves ethanol production Hon, Shuen; Holwerda, Evert K.; Worthen, Robert S. Biotechnology for Biofuels, Vol. 11, Issue 1 https://doi.org/10.1186/s13068-018-1245-2	journal	September 2018
Metabolic and evolutionary responses of Clostridium thermocellum to genetic interventions aimed at improving ethanol production Holwerda, Evert K.; Olson, Daniel G.; Ruppertsberger, Natalie M. Biotechnology for Biofuels, Vol. 13, Issue 1 https://doi.org/10.1186/s13068-020-01680-5	journal	March 2020
Ethanol tolerance in engineered strains of Clostridium thermocellum Olson, Daniel G.; Maloney, Marybeth I.; Lanahan, Anthony A. Biotechnology for Biofuels and Bioproducts, Vol. 16, Issue 1 https://doi.org/10.1186/s13068-023-02379-z	journal	September 2023
Pyrophosphate-free glycolysis in Clostridium thermocellum increases both thermodynamic driving force and ethanol titers Sharma, Bishal Dev; Hon, Shuen; Thusoo, Eashant Biotechnology for Biofuels and Bioproducts, Vol. 17, Issue 1 https://doi.org/10.1186/s13068-024-02591-5	journal	December 2024
Pathway Thermodynamics Highlights Kinetic Obstacles in Central Metabolism Noor, Elad; Bar-Even, Arren; Flamholz, Avi PLoS Computational Biology, Vol. 10, Issue 2 https://doi.org/10.1371/journal.pcbi.1003483	journal	February 2014
Steady-State Metabolite Concentrations Reflect a Balance between Maximizing Enzyme Efficiency and Minimizing Total Metabolite Load Tepper, Naama; Noor, Elad; Amador-Noguez, Daniel PLoS ONE, Vol. 8, Issue 9 https://doi.org/10.1371/journal.pone.0075370	journal	September 2013
Developing a Cell-Free Extract Reaction (CFER) System in Clostridium thermocellum to Identify Metabolic Limitations to Ethanol Production Cui, Jingxuan; Stevenson, David; Korosh, Travis Frontiers in Energy Research, Vol. 8 https://doi.org/10.3389/fenrg.2020.00072	journal	June 2020
NADPH-generating systems in bacteria and archaea Spaans, Sebastiaan K.; Weusthuis, Ruud A.; van der Oost, John Frontiers in Microbiology, Vol. 6 https://doi.org/10.3389/fmicb.2015.00742	journal	July 2015
Flavin-Based Electron Bifurcation, Ferredoxin, Flavodoxin, and Anaerobic Respiration With Protons (Ech) or NAD+ (Rnf) as Electron Acceptors: A Historical Review Buckel, Wolfgang; Thauer, Rudolf K. Frontiers in Microbiology, Vol. 9 https://doi.org/10.3389/fmicb.2018.00401	journal	March 2018
Origin and Evolution of Flavin-Based Electron Bifurcating Enzymes Poudel, Saroj; Dunham, Eric C.; Lindsay, Melody R. Frontiers in Microbiology, Vol. 9 https://doi.org/10.3389/fmicb.2018.01762	journal	August 2018
Distribution, Evolution, Catalytic Mechanism, and Physiological Functions of the Flavin-Based Electron-Bifurcating NADH-Dependent Reduced Ferredoxin: NADP+ Oxidoreductase Liang, Jiyu; Huang, Haiyan; Wang, Shuning Frontiers in Microbiology, Vol. 10 https://doi.org/10.3389/fmicb.2019.00373	journal	March 2019
Obligate autotrophy at the thermodynamic limit of life in a new acetogenic bacterium Frolov, Evgenii N.; Elcheninov, Alexander G.; Gololobova, Alexandra V. Frontiers in Microbiology, Vol. 14 https://doi.org/10.3389/fmicb.2023.1185739	journal	May 2023
Thioredoxin Reductase-Type Ferredoxin: NADP+ Oxidoreductase of Rhodopseudomonas palustris: Potentiometric Characteristics and Reactions with Nonphysiological Oxidants Lesanavičius, Mindaugas; Seo, Daisuke; Čėnas, Narimantas Antioxidants, Vol. 11, Issue 5 https://doi.org/10.3390/antiox11051000	journal	May 2022
Site-Differentiated Iron–Sulfur Cluster Ligation Affects Flavin-Based Electron Bifurcation Activity Wise, Courtney E.; Ledinina, Anastasia E.; Lubner, Carolyn E. Metabolites, Vol. 12, Issue 9 https://doi.org/10.3390/metabo12090823	journal	September 2022

Similar Records

Ferredoxin:NAD⁺ oxidoreductase of Thermoanaerobacterium saccharolyticum and its role in ethanol formation [Identification of a ferredoxin:NAD⁺ oxidoreductase of Thermoanaerobacterium saccharolyticum and its role in ethanol formation]

Journal Article · Fri Sep 30 00:00:00 UTC 2016 · Applied and Environmental Microbiology · OSTI ID:1339505

Tian, Liang; Lo, Jonathan; Shao, Xiongjun; +3 more

Thermodynamics shapes the in vivo enzyme burden of glycolytic pathways

Journal Article · Tue Sep 16 00:00:00 UTC 2025 · mBio · OSTI ID:2588573

Khana, Daven B.; Jen, Annie; Shishkova, Evgenia; +7 more

Structural Characterization and Dynamics of AdhE Ultrastructures from Clostridium thermocellum Show a Containment Strategy for Toxic Intermediates

Journal Article · Fri May 24 00:00:00 UTC 2024 · eLife · OSTI ID:2568050

Ziegler, Samantha; Knott, Brandon; Gruber, Josephine; +6 more

Related Subjects

09 BIOMASS FUELS
FNOR
FNR
biofuel
enzyme
ferredoxin
metabolic engineering
reductase

Title: A distinct class of ferredoxin:NADP+ oxidoreductase enzymes driving thermophilic ethanol production

Citation Formats

References (92)

Similar Records

Related Subjects

Title: A distinct class of ferredoxin:NADP⁺ oxidoreductase enzymes driving thermophilic ethanol production