Protein carbamylation and proteomics: from artifacts to elucidation of biological functions

You, Youngki; Many, Gina; Nakayasu, Ernesto S.

doi:10.3389/frans.2024.1512573

Title: Protein carbamylation and proteomics: from artifacts to elucidation of biological functions

Journal Article · Fri Jan 03 00:00:00 UTC 2025 · Frontiers in Analytical Science

DOI: https://doi.org/10.3389/frans.2024.1512573 · OSTI ID:2571293

You, Youngki ^[1]; Many, Gina ^[1];

^[1]

Pacific Northwest National Laboratory (PNNL), Richland, WA (United States)

Lysine carbamylation is a non-enzymatic protein post-translational modification (PTM) that plays important roles in regulating enzymatic activity and the pathogenesis of diseases such as atherosclerosis, rheumatoid arthritis, and uremia. The progress of understanding the roles of carbamylation in biological systems has been delayed due to lack of systematic assays to study its functions. To aggravate this scenario, carbamylation is a major artifact in proteomics analysis given that urea, which is used during sample preparation, induces carbamylation. In addition, anti-acetyllysine antibodies co-purify carbamylated and acetylated peptides. In a recent paper, we leveraged co-purification with anti-acetyllysine antibodies to develop a method for analyzing carbamylated proteomes. In this perspective article, we discuss how this method may be applied to characterize the physiological functions of carbamylation in humans and other biological models, as well as the utility of establishing novel disease biomarkers.

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Research Organization:: Pacific Northwest National Laboratory (PNNL), Richland, WA (United States)

Sponsoring Organization:: USDOE

Grant/Contract Number:: AC05-76RL01830

OSTI ID:: 2571293

Report Number(s):: PNNL-SA--205105

Journal Information:: Frontiers in Analytical Science, Journal Name: Frontiers in Analytical Science Vol. 4; ISSN 2673-9283

Publisher:: Frontiers Media S.A.Copyright Statement

Country of Publication:: United States

Language:: English

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59 BASIC BIOLOGICAL SCIENCES
carbamylation
disease
function
integrative omics
post-translational modification
proteomics
translational omics

Title: Protein carbamylation and proteomics: from artifacts to elucidation of biological functions

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References (53)

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