Advanced multi-modal mass spectrometry imaging reveals functional differences of placental villous compartments at microscale resolution

Veličković, Marija; Kadam, Leena; Kim, Joonhoon; Zemaitis, Kevin J.; Veličković, Dušan; Gao, Yuqian; Wu, Ruonan; Fillmore, Thomas L.; Orton, Daniel; Williams, Sarah M.; Monroe, Matthew E.; Moore, Ronald J.; Piehowski, Paul D.; Bramer, Lisa M.; Myatt, Leslie; Burnum-Johnson, Kristin E.

doi:10.1038/s41467-025-57107-y

Title: Advanced multi-modal mass spectrometry imaging reveals functional differences of placental villous compartments at microscale resolution

Journal Article · Fri Feb 28 00:00:00 UTC 2025 · Nature Communications

DOI: https://doi.org/10.1038/s41467-025-57107-y · OSTI ID:2566019

^[1]; Kadam, Leena ^[2];

^[1];

^[1]; Veličković, Dušan ^[1];

^[1];

^[1]; Fillmore, Thomas L. ^[1];

^[1]; Williams, Sarah M. ^[1];

^[1];

^[1]; Myatt, Leslie ^[2];

^[1]

Pacific Northwest National Laboratory (PNNL), Richland, WA (United States)
Oregon Health & Science University, Portland, OR (United States)

The placenta is a complex and heterogeneous organ that links the mother and fetus, playing a crucial role in nourishing and protecting the fetus throughout pregnancy. Integrative spatial multi-omics approaches can provide a systems-level understanding of molecular changes underlying the mechanisms leading to the histological variations of the placenta during healthy pregnancy and pregnancy complications. Herein, we advance our metabolome-informed proteome imaging (MIPI) workflow to include lipidomic imaging, while also expanding the molecular coverage of metabolomic imaging by incorporating on-tissue chemical derivatization (OTCD). The improved MIPI workflow advances biomedical investigations by leveraging state-of-the-art molecular imaging technologies. Lipidome imaging identifies molecular differences between two morphologically distinct compartments of a placental villous functional unit, syncytiotrophoblast (STB) and villous core. Next, our advanced metabolome imaging maps villous functional units with enriched metabolomic activities related to steroid and lipid metabolism, outlining distinct molecular distributions across morphologically different villous compartments. Complementary proteome imaging on these villous functional units reveals a plethora of fatty acid- and steroid-related enzymes uniquely distributed in STB and villous core compartments. Integration across our advanced MIPI imaging modalities enables the reconstruction of active biological pathways of molecular synthesis and maternal-fetal signaling across morphologically distinct placental villous compartments with micrometer-scale resolution.

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Research Organization:: Pacific Northwest National Laboratory (PNNL), Richland, WA (United States)

Sponsoring Organization:: USDOE Laboratory Directed Research and Development (LDRD) Program; USDOE Office of Science (SC), Biological and Environmental Research (BER)

Grant/Contract Number:: AC05-76RL01830

OSTI ID:: 2566019

Report Number(s):: PNNL-SA--201502

Journal Information:: Nature Communications, Journal Name: Nature Communications Journal Issue: 1 Vol. 16; ISSN 2041-1723

Publisher:: Nature Publishing GroupCopyright Statement

Country of Publication:: United States

Language:: English

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