Unlocking soybean meal pectin recalcitrance using a multi-enzyme cocktail approach

Plouhinec, Lauriane; Zhang, Liang; Pillon, Alexandre; Haon, Mireille; Grisel, Sacha; Navarro, David; Black, Ian; Neugnot, Virginie; Azadi, Parastoo; Urbanowicz, Breeanna; Berrin, Jean-Guy; Lafond, Mickael

doi:10.1038/s41598-024-83289-4

Title: Unlocking soybean meal pectin recalcitrance using a multi-enzyme cocktail approach

Journal Article · Sat Jan 11 00:00:00 UTC 2025 · Scientific Reports

DOI: https://doi.org/10.1038/s41598-024-83289-4 · OSTI ID:2530545

Plouhinec, Lauriane ^[1]; Zhang, Liang ^[2]; Pillon, Alexandre ^[3]; Haon, Mireille ^[3]; Grisel, Sacha ^[3]; Navarro, David ^[3]; Black, Ian ^[2]; Neugnot, Virginie ^[4]; Azadi, Parastoo ^[2]; Urbanowicz, Breeanna ^[2]; Berrin, Jean-Guy ^[3]; Lafond, Mickael ^[3]

Aix-Marseille Univ., Marseille (France); Adisseo France S.A.S, Toulouse (France)
University of Georgia, Athens, GA (United States)
Aix-Marseille Univ., Marseille (France)
Adisseo France S.A.S, Toulouse (France)

Pectin is a complex plant heteropolysaccharide whose structure and function differ depending on its source. In animal feed, breaking down pectin is essential, as its presence increases feed viscosity and reduces nutrient absorption. Soybean meal, a protein-rich poultry feed ingredient, contains significant amounts of pectin, the structure of which remains unclear. Consequently, the enzyme activities required to degrade soybean meal pectin and how they interact are still open questions. In this study, we produced 15 recombinant fungal carbohydrate-active enzymes (CAZymes) identified from fungal secretomes acting on pectin. After observing that these enzymes were not active on soybean meal pectin when used alone, we developed a semi-miniaturized method to evaluate their effect as multi-activity cocktails. We designed and tested 12 enzyme pools, containing up to 15 different CAZymes, using several hydrolysis markers. Thanks to our multiactivity enzymatic approach combined with a Pearson correlation matrix, we identified 10 fungal CAZymes efficient on soybean meal pectin, 9 of which originate from Talaromyces versatilis. Based on enzyme specificity and linkage analysis, we propose a structural model for soybean meal pectin. Our findings underscore the importance of combining CAZymes to improve the degradation of agricultural co-products.

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Research Organization:: University of Georgia, Athens, GA (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Basic Energy Sciences (BES); USDOE Office of Science (SC), Biological and Environmental Research (BER)

Grant/Contract Number:: SC0015662; SC0023223

OSTI ID:: 2530545

Journal Information:: Scientific Reports, Journal Name: Scientific Reports Journal Issue: 1 Vol. 15; ISSN 2045-2322

Publisher:: Nature Publishing GroupCopyright Statement

Country of Publication:: United States

Language:: English

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60 APPLIED LIFE SCIENCES
Animal feed
Biochemistry
Biotechnology
CAZymes
Filamentous fungi
Microbiology
Multi-activity enzymatic cocktails
Rhamnogalacturonan
Soybean meal pectin

Title: Unlocking soybean meal pectin recalcitrance using a multi-enzyme cocktail approach

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