Impact of Oxalic Acid Consumption and pH on the In Vitro Biological Control of Oxalogenic Phytopathogen Sclerotinia sclerotiorum

Estoppey, Aislinn; Vallat-Michel, Armelle; Chain, Patrick S.; Bindschedler, Saskia; Junier, Pilar

doi:10.3390/jof11030191

Title: Impact of Oxalic Acid Consumption and pH on the In Vitro Biological Control of Oxalogenic Phytopathogen Sclerotinia sclerotiorum

Journal Article · Sun Mar 02 00:00:00 UTC 2025 · Journal of Fungi

DOI: https://doi.org/10.3390/jof11030191 · OSTI ID:2526267

; Vallat-Michel, Armelle;

; Bindschedler, Saskia;

The phytopathogenic fungus Sclerotinia sclerotiorum has a wide host range and causes significant economic losses in crops worldwide. This pathogen uses oxalic acid as a virulence factor; for this reason, the degradation of this organic acid by oxalotrophic bacteria has been proposed as a biological control approach. However, previous studies on the potential role of oxalotrophy in biocontrol did not investigate the differential effect of oxalic acid consumption and the subsequent pH alkalinisation on fungal growth. In this study, confrontation experiments on different media using a wild-type (WT) strain of S. sclerotiorum and an oxalate-deficient mutant (strain Δoah) with the soil oxalotrophic bacteria Cupriavidus necator and Cupriavidus oxalaticus showed the combined effect of media composition on oxalic acid production, pH, and fungal growth control. Oxalotrophic bacteria were able to control S. sclerotiorum only in the medium in which oxalic acid was produced. However, the deficient Δoah mutant was also controlled, indicating that the consumption of oxalic acid is not the sole mechanism of biocontrol. WT S. sclerotiorum acidified the medium when inoculated alone, while for both fungi, the pH of the medium changed from neutral to alkaline in the presence of bacteria. Therefore, medium alkalinisation independent of oxalotrophy contributes to fungal growth control.

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Sponsoring Organization:: USDOE

OSTI ID:: 2526267

Journal Information:: Journal of Fungi, Journal Name: Journal of Fungi Journal Issue: 3 Vol. 11; ISSN 2309-608X; ISSN JFOUCU

Publisher:: MDPI AGCopyright Statement

Country of Publication:: Switzerland

Language:: English

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