Multi-slice electron ptychographic tomography for three-dimensional phase-contrast microscopy beyond the depth of focus limits

Romanov, Andrey; Cho, Min Gee; Scott, Mary Cooper; Pelz, Philipp

doi:10.1088/2515-7639/ad9ad2

Title: Multi-slice electron ptychographic tomography for three-dimensional phase-contrast microscopy beyond the depth of focus limits

Journal Article · Mon Dec 16 00:00:00 UTC 2024 · JPhys Materials

DOI: https://doi.org/10.1088/2515-7639/ad9ad2 · OSTI ID:2496631

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Freidrich Alexander Univ. Erlangen-Nürnberg (FAU), Erlangen (Germany)
Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States). Molecular Foundry; University of California, Berkeley, CA (United States)

Electron ptychography is a powerful computational method for atomic-resolution imaging with high contrast for weakly and strongly scattering elements. Modern algorithms coupled with fast and efficient detectors allow imaging specimens with tens of nanometers thicknesses with sub-0.5 Ångstrom lateral resolution. However, the axial resolution in these approaches is currently limited to a few nanometers, limiting their ability to solve novel atomic structures ab initio. Here, we experimentally demonstrate multi-slice ptychographic electron tomography, which allows atomic resolution three-dimensional phase-contrast imaging in a volume surpassing the depth of field limits. We reconstruct tilt-series 4D-STEM measurements of a $$\mathrm{Co_3O_4}$$ nanocube, yielding 2 Å axial and 0.7 Å transverse resolution in a reconstructed volume of $$\mathrm{(18.2\,nm)^3}$$. Our results demonstrate a 13.5-fold improvement in axial resolution compared to multi-slice ptychography while retaining the atomic lateral resolution and the capability to image volumes beyond the depth of field limit. Multi-slice ptychographic electron tomography significantly expands the volume of materials accessible using high-resolution electron microscopy. We discuss further experimental and algorithmic improvements necessary to also resolve single weakly scattering atoms in 3D.

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Research Organization:: Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Basic Energy Sciences (BES). Scientific User Facilities (SUF)

Grant/Contract Number:: AC02-05CH11231

OSTI ID:: 2496631

Journal Information:: JPhys Materials, Journal Name: JPhys Materials Journal Issue: 1 Vol. 8; ISSN 2515-7639

Publisher:: IOP PublishingCopyright Statement

Country of Publication:: United States

Language:: English

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