ToF-SIMS spectral analysis of Shewanella oneidensis MR-1 biofilms
[1];
[4]
- Univ. of Illinois, Chicago, IL (United States); Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States)
- Pacific Northwest National Laboratory (PNNL), Richland, WA (United States)
- Univ. of Illinois, Chicago, IL (United States)
- Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States)
Analysis of bacterial biofilms is particularly challenging and important with diverse applications from systems biology to biotechnology. Among the variety of techniques that have been applied, time-of-flight secondary ion mass spectrometry (ToF-SIMS) has many powerful features in studying the surface characteristics of biofilms. ToF-SIMS offers high spatial resolution, mass resolution, and mass accuracy, which permit surface sensitive analysis of biofilm components. Thus, ToF-SIMS provides a powerful solution to addressing the challenge of bacterial biofilm analysis. This dataset covers ToF-SIMS analysis of Shewanella oneidensis MR-1 isolated from freshwater lake sediment in New York state. The MR-1 strain is known to have metal and sulfur reducing properties and it can be used for bioremediation and wastewater treatment. There is a current need to identify small molecules and fragments produced from bacterial biofilms, especially those from extracellular polymeric substance (EPS). Static ToF-SIMS spectra of MR-1 were obtained using an IONTOF TOF.SIMS V instrument equipped with a 25 keV Bi$$^+_3$$ metal ion gun. Identified molecules and molecular fragments are compared against known biological databases and the reported peaks have at least 65 ppm mass accuracy. These molecules range from lipids, fatty acids, flavonoids, and quinolones to other naturally occurring organic compounds. It is anticipated that the mass spectral identification of key peaks will assist detection of metabolites, EPS molecules like polysaccharides, and biologically relevant small organic molecules using ToF-SIMS in future surface and interface research.
- Research Organization:
- Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States); Pacific Northwest National Laboratory (PNNL), Richland, WA (United States)
- Sponsoring Organization:
- USDOE; USDOE Laboratory Directed Research and Development (LDRD) Program
- Grant/Contract Number:
- AC05-00OR22725; AC05-76RL01830
- OSTI ID:
- 2484260
- Report Number(s):
- PNNL-SA--203069
- Journal Information:
- Results in surfaces and interfaces (Online), Journal Name: Results in surfaces and interfaces (Online) Vol. 18; ISSN 2666-8459
- Publisher:
- ElsevierCopyright Statement
- Country of Publication:
- United States
- Language:
- English
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