Directing Nanoparticle Organization in Response to Diverse Chemical Inputs

Xiong, Yan; Yancey, Colin; Lee, Heon-Joon; Lee, Dayoung Gloria; Helm, Emily; Kang, Byunghwa; Grinthal, Alison; McKeen, Daniel; Gang, Oleg; Schulman, Rebecca

doi:10.1021/jacs.4c07259

Title: Directing Nanoparticle Organization in Response to Diverse Chemical Inputs

Journal Article · 29 October 2024 · Journal of the American Chemical Society

DOI: https://doi.org/10.1021/jacs.4c07259 · OSTI ID:2480273

Xiong, Yan ^[1]; Yancey, Colin ^[1]; Lee, Heon-Joon ^[1];

^[2]; Helm, Emily ^[1]; Kang, Byunghwa ^[1]; Grinthal, Alison ^[1]; McKeen, Daniel ^[2];

^[3];

^[1]

Johns Hopkins Univ., Baltimore, MD (United States)
Columbia Univ., New York, NY (United States)
Columbia Univ., New York, NY (United States); Brookhaven National Laboratory (BNL), Upton, NY (United States). Center for Functional Nanomaterials (CFN)

Signaling cascades are crucial for transducing stimuli in biological systems, enabling multiple stimuli to regulate a downstream target with precisely controlled timing and amplifying signals through a series of intermediary reactions. Developing a robust signaling system with such capabilities would be pivotal for programming complex behaviors in synthetic DNA-based molecular devices. However, although “software” such as nucleic acid circuits could potentially be harnessed to relay signals to DNA-based nanostructure hardware, such explorations have been limited. Here, in this study, we develop a platform for transducing a variety of stimuli via messenger-mediated reactions to regulate the release and reloading of gold nanoparticles (AuNPs) in a 3D DNA framework. In the first step, an in vitro transcription circuit is engineered to sense and amplify chemical stimuli, including arbitrary DNA sequences and proteins, producing RNA. In the second step, the RNA releases the DNA-coated AuNPs from the DNA framework via a strand displacement reaction. AuNP reloading is controlled by a separate step driven by degradation of the RNA. Our platform holds promise for applications requiring dynamic multiagent control over DNA-based devices, offering a versatile tool for advanced molecular device engineering.

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Research Organization:: Brookhaven National Laboratory (BNL), Upton, NY (United States)

Sponsoring Organization:: National Institutes of Health (NIH); National Science Foundation (NSF); US Army Research Office (ARO); USDOE Office of Science (SC), Basic Energy Sciences (BES). Scientific User Facilities (SUF)

Grant/Contract Number:: SC0012704

OSTI ID:: 2480273

Report Number(s):: BNL--226392-2024-JAAM

Journal Information:: Journal of the American Chemical Society, Journal Name: Journal of the American Chemical Society Journal Issue: 45 Vol. 146; ISSN 0002-7863

Publisher:: American Chemical Society (ACS)Copyright Statement

Country of Publication:: United States

Language:: English

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