Microbial functional diversity across biogeochemical provinces in the central Pacific Ocean

Saunders, Jaclyn K.; McIlvin, Matthew R.; Dupont, Chris L.; Kaul, Drishti; Moran, Dawn M.; Horner, Tristan; Laperriere, Sarah M.; Webb, Eric A.; Bosak, Tanja; Santoro, Alyson E.; Saito, Mak A.

doi:10.1073/pnas.2200014119

Title: Microbial functional diversity across biogeochemical provinces in the central Pacific Ocean

Journal Article · 06 September 2022 · Proceedings of the National Academy of Sciences of the United States of America

DOI: https://doi.org/10.1073/pnas.2200014119 · OSTI ID:2471115

^[1]; McIlvin, Matthew R. ^[2]; Dupont, Chris L. ^[3];

^[3]; Moran, Dawn M. ^[2]; Horner, Tristan ^[2]; Laperriere, Sarah M. ^[4];

^[5]; Bosak, Tanja ^[6];

^[4];

^[2]

Woods Hole Oceanographic Institution, MA (United States); University of Georgia, Athens, GA (United States)
Woods Hole Oceanographic Institution, MA (United States)
J. C. Venter Institute, La Jolla, CA (United States)
University of California, Santa Barbara, CA (United States)
University of Southern California, Los Angeles, CA (United States)
Massachusetts Institute of Technology (MIT), Cambridge, MA (United States)

Enzymes catalyze key reactions within Earth’s life-sustaining biogeochemical cycles. Here, we use metaproteomics to examine the enzymatic capabilities of the microbial community (0.2 to 3 µm) along a 5,000-km-long, 1-km-deep transect in the central Pacific Ocean. Eighty-five percent of total protein abundance was of bacterial origin, with Archaea contributing 1.6%. Over 2,000 functional KEGG Ontology (KO) groups were identified, yet only 25 KO groups contributed over half of the protein abundance, simultaneously indicating abundant key functions and a long tail of diverse functions. Vertical attenuation of individual proteins displayed stratification of nutrient transport, carbon utilization, and environmental stress. The microbial community also varied along horizontal scales, shaped by environmental features specific to the oligotrophic North Pacific Subtropical Gyre, the oxygen-depleted Eastern Tropical North Pacific, and nutrient-rich equatorial upwelling. Some of the most abundant proteins were associated with nitrification and C1 metabolisms, with observed interactions between these pathways. The oxidoreductases nitrite oxidoreductase (NxrAB), nitrite reductase (NirK), ammonia monooxygenase (AmoABC), manganese oxidase (MnxG), formate dehydrogenase (FdoGH and FDH), and carbon monoxide dehydrogenase (CoxLM) displayed distributions indicative of biogeochemical status such as oxidative or nutritional stress, with the potential to be more sensitive than chemical sensors. Enzymes that mediate transformations of atmospheric gases like CO, CO₂, NO, methanethiol, and methylamines were most abundant in the upwelling region. Here, we identified hot spots of biochemical transformation in the central Pacific Ocean, highlighted previously understudied metabolic pathways in the environment, and provided rich empirical data for biogeochemical models critical for forecasting ecosystem response to climate change.

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Research Organization:: USDOE Joint Genome Institute (JGI), Walnut Creek, CA (United States)

Sponsoring Organization:: Gordon and Betty Moore Foundation; National Institutes of Health (NIH); National Science Foundation (NSF); USDOE Office of Science (SC), Biological and Environmental Research (BER)

Grant/Contract Number:: AC02-05CH11231

OSTI ID:: 2471115

Journal Information:: Proceedings of the National Academy of Sciences of the United States of America, Journal Name: Proceedings of the National Academy of Sciences of the United States of America Journal Issue: 37 Vol. 119; ISSN 0027-8424

Publisher:: National Academy of SciencesCopyright Statement

Country of Publication:: United States

Language:: English

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54 ENVIRONMENTAL SCIENCES
biological sciences
environmental sciences
marine microbial ecology
mesopelagic
metaproteomics
methylotrophy
nitrification
science & technology

Title: Microbial functional diversity across biogeochemical provinces in the central Pacific Ocean

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