Metagenome-assembled genomes provide insight into the metabolic potential during early production of Hydraulic Fracturing Test Site 2 in the Delaware Basin

Stemple, Brooke; Gulliver, Djuna; Sarkar, Preom; Tinker, Kara; Bibby, Kyle

doi:10.3389/fmicb.2024.1376536

Title: Metagenome-assembled genomes provide insight into the metabolic potential during early production of Hydraulic Fracturing Test Site 2 in the Delaware Basin

Journal Article · Wed Jun 12 00:00:00 UTC 2024 · Frontiers in Microbiology

DOI: https://doi.org/10.3389/fmicb.2024.1376536 · OSTI ID:2468647

Stemple, Brooke ^[1]; Gulliver, Djuna ^[2]; Sarkar, Preom ^[3]; Tinker, Kara ^[4]; Bibby, Kyle ^[1]

University of Notre Dame, IN (United States); Oak Ridge Institute for Science and Education (ORISE), Oak Ridge, TN (United States)
National Energy Technology Lab. (NETL), Pittsburgh, PA (United States)
Oak Ridge Institute for Science and Education (ORISE), Oak Ridge, TN (United States); National Energy Technology Lab. (NETL), Pittsburgh, PA (United States)
National Energy Technology Lab. (NETL), Pittsburgh, PA (United States); Leidos Research Support Team, Pittsburgh, PA (United States)

Demand for natural gas continues to climb in the United States, having reached a record monthly high of 104.9 billion cubic feet per day (Bcf/d) in November 2023. Hydraulic fracturing, a technique used to extract natural gas and oil from deep underground reservoirs, involves injecting large volumes of fluid, proppant, and chemical additives into shale units. This is followed by a “shut-in” period, during which the fracture fluid remains pressurized in the well for several weeks. The microbial processes that occur within the reservoir during this shut-in period are not well understood; yet, these reactions may significantly impact the structural integrity and overall recovery of oil and gas from the well. To shed light on this critical phase, we conducted an analysis of both pre-shut-in material alongside production fluid collected throughout the initial production phase at the Hydraulic Fracturing Test Site 2 (HFTS 2) located in the prolific Wolfcamp formation within the Permian Delaware Basin of west Texas, USA. Specifically, we aimed to assess the microbial ecology and functional potential of the microbial community during this crucial time frame. Prior analysis of 16S rRNA sequencing data through the first 35 days of production revealed a strong selection for a Clostridia species corresponding to a significant decrease in microbial diversity. Here, we performed a metagenomic analysis of produced water sampled on Day 33 of production. This analysis yielded three high-quality metagenome-assembled genomes (MAGs), one of which was a Clostridia draft genome closely related to the recently classified Petromonas tenebris. This draft genome likely represents the dominant Clostridia species observed in our 16S rRNA profile. Annotation of the MAGs revealed the presence of genes involved in critical metabolic processes, including thiosulfate reduction, mixed acid fermentation, and biofilm formation. These findings suggest that this microbial community has the potential to contribute to well souring, biocorrosion, and biofouling within the reservoir. Our research provides unique insights into the early stages of production in one of the most prolific unconventional plays in the United States, with important implications for well management and energy recovery.

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Research Organization:: National Energy Technology Laboratory (NETL), Pittsburgh, PA, Morgantown, WV, and Albany, OR (United States)

Sponsoring Organization:: USDOE Office of Fossil Energy and Carbon Management (FECM), Office of Resource Sustainability

OSTI ID:: 2468647

Journal Information:: Frontiers in Microbiology, Journal Name: Frontiers in Microbiology Vol. 15; ISSN 1664-302X

Publisher:: Frontiers Research FoundationCopyright Statement

Country of Publication:: United States

Language:: English

References (82)

Bacterial Communities Associated with Production Facilities of Two Newly Drilled Thermogenic Natural Gas Wells in the Barnett Shale (Texas, USA) Davis, James P.; Struchtemeyer, Christopher G.; Elshahed, Mostafa S. Microbial Ecology, Vol. 64, Issue 4 https://doi.org/10.1007/s00248-012-0073-3	journal	May 2012
Biological souring and mitigation in oil reservoirs Gieg, Lisa M.; Jack, Tom R.; Foght, Julia M. Applied Microbiology and Biotechnology, Vol. 92, Issue 2 https://doi.org/10.1007/s00253-011-3542-6	journal	August 2011
Quantitative real-time PCR using TaqMan and SYBR Green for Actinobacillus actinomycetemcomitans , Porphyromonas gingivalis , Prevotella intermedia , tetQ gene and total bacteria Maeda, Hiroshi; Fujimoto, Chiyo; Haruki, Yasuhiro FEMS Immunology & Medical Microbiology, Vol. 39, Issue 1 https://doi.org/10.1016/S0928-8244(03)00224-4	journal	October 2003
Molecular characterization of methanogenic N 5 -methyl-tetrahydromethanopterin: Coenzyme M methyltransferase Upadhyay, Vikrant; Ceh, Katharina; Tumulka, Franz Biochimica et Biophysica Acta (BBA) - Biomembranes, Vol. 1858, Issue 9 https://doi.org/10.1016/j.bbamem.2016.06.011	journal	September 2016
Bacterial diguanylate cyclases: Structure, function and mechanism in exopolysaccharide biofilm development Whiteley, Chris G.; Lee, Duu-Jong Biotechnology Advances, Vol. 33, Issue 1 https://doi.org/10.1016/j.biotechadv.2014.11.010	journal	January 2015
Tolerance of Clostridium perfringens biofilms to disinfectants commonly used in the food industry Charlebois, Audrey; Jacques, Mario; Boulianne, Martine Food Microbiology, Vol. 62 https://doi.org/10.1016/j.fm.2016.09.009	journal	April 2017
A critical assessment of the efficacy of biocides used during the hydraulic fracturing process in shale natural gas wells Struchtemeyer, Christopher G.; Morrison, Michael D.; Elshahed, Mostafa S. International Biodeterioration & Biodegradation, Vol. 71 https://doi.org/10.1016/j.ibiod.2012.01.013	journal	July 2012
Diversity analyses of microbial communities in petroleum samples from Brazilian oil fields Silva, T. R.; Verde, L. C. L.; Santos Neto, E. V. International Biodeterioration & Biodegradation, Vol. 81 https://doi.org/10.1016/j.ibiod.2012.05.005	journal	July 2013
Phylogenetic diversity of microbial communities associated with the crude-oil, large-insoluble-particle and formation-water components of the reservoir fluid from a non-flooded high-temperature petroleum reservoir Kobayashi, Hajime; Endo, Keita; Sakata, Susumu Journal of Bioscience and Bioengineering, Vol. 113, Issue 2 https://doi.org/10.1016/j.jbiosc.2011.09.015	journal	February 2012
Physical, chemical, and biological characteristics of compounds used in hydraulic fracturing Stringfellow, William T.; Domen, Jeremy K.; Camarillo, Mary Kay Journal of Hazardous Materials, Vol. 275 https://doi.org/10.1016/j.jhazmat.2014.04.040	journal	June 2014
Role of polysaccharides in Pseudomonas aeruginosa biofilm development Ryder, Cynthia; Byrd, Matthew; Wozniak, Daniel J. Current Opinion in Microbiology, Vol. 10, Issue 6 https://doi.org/10.1016/j.mib.2007.09.010	journal	December 2007
Oil reservoirs, an exceptional habitat for microorganisms Pannekens, Mark; Kroll, Lisa; Müller, Hubert New Biotechnology, Vol. 49 https://doi.org/10.1016/j.nbt.2018.11.006	journal	March 2019
rdlA, a new gene encoding a rhodanese-like protein in Halanaerobium congolense and other thiosulfate-reducing anaerobes Ravot, Gilles; Casalot, Laurence; Ollivier, Bernard Research in Microbiology, Vol. 156, Issue 10 https://doi.org/10.1016/j.resmic.2005.05.009	journal	December 2005
Geochemical and sulfate isotopic evolution of flowback and produced waters reveals water-rock interactions following hydraulic fracturing of a tight hydrocarbon reservoir Osselin, F.; Saad, S.; Nightingale, M. Science of The Total Environment, Vol. 687 https://doi.org/10.1016/j.scitotenv.2019.07.066	journal	October 2019
Methanogenic Archaea in Marcellus Shale: A Possible Mechanism for Enhanced Gas Recovery in Unconventional Shale Resources Tucker, Yael Tarlovsky; Kotcon, James; Mroz, Thomas Environmental Science & Technology, Vol. 49, Issue 11 https://doi.org/10.1021/acs.est.5b00765	journal	May 2015
Biogenic versus Thermogenic H ₂ S Source Determination in Bakken Wells: Considerations for Biocide Application Gaspar, Jason; Davis, Drew; Camacho, Carlos Environmental Science & Technology Letters, Vol. 3, Issue 4 https://doi.org/10.1021/acs.estlett.6b00075	journal	March 2016
Biogeochemistry of the Antrim Shale Natural Gas Reservoir Stemple, Brooke; Tinker, Kara; Sarkar, Preom ACS Earth and Space Chemistry, Vol. 5, Issue 7 https://doi.org/10.1021/acsearthspacechem.1c00087	journal	June 2021
Microbial Community Changes in Hydraulic Fracturing Fluids and Produced Water from Shale Gas Extraction Murali Mohan, Arvind; Hartsock, Angela; Bibby, Kyle J. Environmental Science & Technology, Vol. 47, Issue 22 https://doi.org/10.1021/es402928b	journal	October 2013
A Critical Review of the Risks to Water Resources from Unconventional Shale Gas Development and Hydraulic Fracturing in the United States Vengosh, Avner; Jackson, Robert B.; Warner, Nathaniel Environmental Science & Technology, Vol. 48, Issue 15 https://doi.org/10.1021/es405118y	journal	March 2014
Temporal Changes in Microbial Ecology and Geochemistry in Produced Water from Hydraulically Fractured Marcellus Shale Gas Wells Cluff, Maryam A.; Hartsock, Angela; MacRae, Jean D. Environmental Science & Technology, Vol. 48, Issue 11 https://doi.org/10.1021/es501173p	journal	May 2014
Biocides in Hydraulic Fracturing Fluids: A Critical Review of Their Usage, Mobility, Degradation, and Toxicity Kahrilas, Genevieve A.; Blotevogel, Jens; Stewart, Philip S. Environmental Science & Technology, Vol. 49, Issue 1 https://doi.org/10.1021/es503724k	journal	December 2014
Microbial Dynamics and Control in Shale Gas Production Gaspar, Jason; Mathieu, Jacques; Yang, Yu Environmental Science & Technology Letters, Vol. 1, Issue 12 https://doi.org/10.1021/ez5003242	journal	November 2014
Ultra-high-throughput microbial community analysis on the Illumina HiSeq and MiSeq platforms Caporaso, J. Gregory; Lauber, Christian L.; Walters, William A. The ISME Journal, Vol. 6, Issue 8 https://doi.org/10.1038/ismej.2012.8	journal	March 2012
Succession in the petroleum reservoir microbiome through an oil field production lifecycle Vigneron, Adrien; Alsop, Eric B.; Lomans, Bartholomeus P. The ISME Journal, Vol. 11, Issue 9 https://doi.org/10.1038/ismej.2017.78	journal	May 2017
Structure of a methyl-coenzyme M reductase from Black Sea mats that oxidize methane anaerobically Shima, Seigo; Krueger, Martin; Weinert, Tobias Nature, Vol. 481, Issue 7379 https://doi.org/10.1038/nature10663	journal	November 2011
Fast gapped-read alignment with Bowtie 2 Langmead, Ben; Salzberg, Steven L. Nature Methods, Vol. 9, Issue 4 https://doi.org/10.1038/nmeth.1923	journal	March 2012
Microbial metabolisms in a 2.5-km-deep ecosystem created by hydraulic fracturing in shales Daly, Rebecca A.; Borton, Mikayla A.; Wilkins, Michael J. Nature Microbiology, Vol. 1, Issue 10 https://doi.org/10.1038/nmicrobiol.2016.146	journal	September 2016
Reproducible, interactive, scalable and extensible microbiome data science using QIIME 2 Bolyen, Evan; Rideout, Jai Ram; Dillon, Matthew R. Nature Biotechnology, Vol. 37, Issue 8 https://doi.org/10.1038/s41587-019-0209-9	journal	July 2019
Novel clostridial lineages recovered from metagenomes of a hot oil reservoir Christman, Glenn D.; León-Zayas, Rosa I.; Zhao, Rui Scientific Reports, Vol. 10, Issue 1 https://doi.org/10.1038/s41598-020-64904-6	journal	May 2020
RASTtk: A modular and extensible implementation of the RAST algorithm for building custom annotation pipelines and annotating batches of genomes Brettin, Thomas; Davis, James J.; Disz, Terry Scientific Reports, Vol. 5, Issue 1 https://doi.org/10.1038/srep08365	journal	February 2015
Global patterns of 16S rRNA diversity at a depth of millions of sequences per sample Caporaso, J. G.; Lauber, C. L.; Walters, W. A. Proceedings of the National Academy of Sciences, Vol. 108, Issue Supplement_1 https://doi.org/10.1073/pnas.1000080107	journal	June 2010
Hydrogenotrophic methanogenesis in archaeal phylum Verstraetearchaeota reveals the shared ancestry of all methanogens Berghuis, Bojk A.; Yu, Feiqiao Brian; Schulz, Frederik Proceedings of the National Academy of Sciences, Vol. 116, Issue 11 https://doi.org/10.1073/pnas.1815631116	journal	February 2019
Oil reservoir biofouling control Bakke, Rune; Rivedal, Bergny; Mehan, Surinder Biofouling, Vol. 6, Issue 1 https://doi.org/10.1080/08927019209386209	journal	July 1992
IDBA-UD: a de novo assembler for single-cell and metagenomic sequencing data with highly uneven depth Peng, Y.; Leung, H. C. M.; Yiu, S. M. Bioinformatics, Vol. 28, Issue 11 https://doi.org/10.1093/bioinformatics/bts174	journal	April 2012
QUAST: quality assessment tool for genome assemblies Gurevich, Alexey; Saveliev, Vladislav; Vyahhi, Nikolay Bioinformatics, Vol. 29, Issue 8 https://doi.org/10.1093/bioinformatics/btt086	journal	February 2013
GTDB-Tk: a toolkit to classify genomes with the Genome Taxonomy Database Chaumeil, Pierre-Alain; Mussig, Aaron J.; Hugenholtz, Philip Bioinformatics https://doi.org/10.1093/bioinformatics/btz848	journal	November 2019
Hydraulic fracturing offers view of microbial life in the deep terrestrial subsurface Mouser, Paula J.; Borton, Mikayla; Darrah, Thomas H. FEMS Microbiology Ecology, Vol. 92, Issue 11 https://doi.org/10.1093/femsec/fiw166	journal	August 2016
Microbial communities in Bakken region produced water Lipus, Daniel; Roy, Dhritikshama; Khan, Eakalak FEMS Microbiology Letters, Vol. 365, Issue 12 https://doi.org/10.1093/femsle/fny107	journal	April 2018
DRAM for distilling microbial metabolism to automate the curation of microbiome function Shaffer, Michael; Borton, Mikayla A.; McGivern, Bridget B. Nucleic Acids Research, Vol. 48, Issue 16 https://doi.org/10.1093/nar/gkaa621	journal	August 2020
KEGG: integrating viruses and cellular organisms Kanehisa, Minoru; Furumichi, Miho; Sato, Yoko Nucleic Acids Research, Vol. 49, Issue D1 https://doi.org/10.1093/nar/gkaa970	journal	October 2020
The SILVA ribosomal RNA gene database project: improved data processing and web-based tools Quast, Christian; Pruesse, Elmar; Yilmaz, Pelin Nucleic Acids Research, Vol. 41, Issue D1 https://doi.org/10.1093/nar/gks1219	journal	November 2012
The SILVA and “All-species Living Tree Project (LTP)” taxonomic frameworks Yilmaz, Pelin; Parfrey, Laura Wegener; Yarza, Pablo Nucleic Acids Research, Vol. 42, Issue D1 https://doi.org/10.1093/nar/gkt1209	journal	November 2013
Caminicella sporogenes gen. nov., sp. nov., a novel thermophilic spore-forming bacterium isolated from an East-Pacific Rise hydrothermal vent. Alain, Karine; Pignet, Patricia; Zbinden, Magali International Journal of Systematic and Evolutionary Microbiology, Vol. 52, Issue 5 https://doi.org/10.1099/00207713-52-5-1621	journal	September 2002
Algoriphagus machipongonensis sp. nov., co-isolated with a colonial choanoflagellate Alegado, Rosanna A.; Grabenstatter, Jonathan D.; Zuzow, Richard International Journal of Systematic and Evolutionary Microbiology, Vol. 63, Issue Pt_1 https://doi.org/10.1099/ijs.0.038646-0	journal	January 2013
CheckM: assessing the quality of microbial genomes recovered from isolates, single cells, and metagenomes Parks, Donovan H.; Imelfort, Michael; Skennerton, Connor T. Genome Research, Vol. 25, Issue 7 https://doi.org/10.1101/gr.186072.114	journal	May 2015
Comparative genomics and physiology of the genus Methanohalophilus , a prevalent methanogen in hydraulically fractured shale Borton, Mikayla A.; Daly, Rebecca A.; O'Banion, Bridget Environmental Microbiology, Vol. 20, Issue 12 https://doi.org/10.1111/1462-2920.14467	journal	December 2018
Microbial communities in flowback water impoundments from hydraulic fracturing for recovery of shale gas Murali Mohan, Arvind; Hartsock, Angela; Hammack, Richard W. FEMS Microbiology Ecology, Vol. 86, Issue 3 https://doi.org/10.1111/1574-6941.12183	journal	August 2013
Microbial community analyses of produced waters from high-temperature oil reservoirs reveal unexpected similarity between geographically distant oil reservoirs Kim, Daehyun D.; O'Farrell, Corynne; Toth, Courtney R. A. Microbial Biotechnology, Vol. 11, Issue 4 https://doi.org/10.1111/1751-7915.13281	journal	May 2018
Environmental signals and regulatory pathways that influence biofilm formation: Signalling in biofilm formation Stanley, Nicola R.; Lazazzera, Beth A. Molecular Microbiology, Vol. 52, Issue 4 https://doi.org/10.1111/j.1365-2958.2004.04036.x	journal	April 2004
Properties of the two isoenzymes of methyl-coenzyme M reductase in Methanobacterium thermoautotrophicum Bonacker, Lutz G.; Baudner, Siegfried; Morschel, Erhard European Journal of Biochemistry, Vol. 217, Issue 2 https://doi.org/10.1111/j.1432-1033.1993.tb18281.x	journal	October 1993
Formylmethanofuran dehydrogenases from methanogenic Archaea Substrate specificity, EPR properties and reversible inactivation by cyanide of the molybdenum or tungsten iron-sulfur proteins Bertram, Peter A.; Karrasch, Marion; Schmitz, Ruth A. European Journal of Biochemistry, Vol. 220, Issue 2 https://doi.org/10.1111/j.1432-1033.1994.tb18646.x	journal	March 1994
Bacterial communities associated with hydraulic fracturing fluids in thermogenic natural gas wells in North Central Texas, USA Struchtemeyer, Christopher G.; Elshahed, Mostafa S. FEMS Microbiology Ecology, Vol. 81, Issue 1 https://doi.org/10.1111/j.1574-6941.2011.01196.x	journal	November 2011
A radical-chemical route to acetyl-CoA: the anaerobically induced pyruvate formate-lyase system of Escherichia coli Knappe, Joachim; Sawers, Gary FEMS Microbiology Letters, Vol. 75, Issue 4 https://doi.org/10.1111/j.1574-6968.1990.tb04108.x	journal	August 1990
Deep-Subsurface Pressure Stimulates Metabolic Plasticity in Shale-Colonizing Halanaerobium spp. Booker, Anne E.; Hoyt, David W.; Meulia, Tea Applied and Environmental Microbiology, Vol. 85, Issue 12 https://doi.org/10.1128/AEM.00018-19	journal	April 2019
Genetics and Physiology of Acetate Metabolism by the Pta-Ack Pathway of Streptococcus mutans Kim, Jeong Nam; Ahn, Sang-Joon; Burne, Robert A. Applied and Environmental Microbiology, Vol. 81, Issue 15 https://doi.org/10.1128/AEM.01160-15	journal	August 2015
Predominance and Metabolic Potential of Halanaerobium spp. in Produced Water from Hydraulically Fractured Marcellus Shale Wells Lipus, Daniel; Vikram, Amit; Ross, Daniel Applied and Environmental Microbiology, Vol. 83, Issue 8 https://doi.org/10.1128/AEM.02659-16	journal	February 2017
Multiple Factors Modulate Biofilm Formation by the Anaerobic Pathogen Clostridium difficile Thapa, T.; Leuzzi, R.; Ng, Y. K. Journal of Bacteriology, Vol. 195, Issue 3 https://doi.org/10.1128/JB.01980-12	journal	November 2012
Thiosulfate reduction, an important physiological feature shared by members of the order thermotogales. Ravot, G.; Ollivier, B.; Magot, M. Applied and environmental microbiology, Vol. 61, Issue 5 https://doi.org/10.1128/aem.61.5.2053-2055.1995	journal	January 1995
Cloning, sequencing, and expression of genes encoding phosphotransacetylase and acetate kinase from Clostridium acetobutylicum ATCC 824 Boynton, Z. L.; Bennett, G. N.; Rudolph, F. B. Applied and Environmental Microbiology, Vol. 62, Issue 8 https://doi.org/10.1128/aem.62.8.2758-2766.1996	journal	August 1996
Draft Genome Sequence of Methanohalophilus mahii Strain DAL1 Reconstructed from a Hydraulic Fracturing-Produced Water Metagenome Lipus, Daniel; Vikram, Amit; Ross, Daniel E. Genome Announcements, Vol. 4, Issue 5 https://doi.org/10.1128/genomea.00899-16	journal	September 2016
Characterization of anaerobic sulfite reduction by Salmonella typhimurium and purification of the anaerobically induced sulfite reductase. Hallenbeck, P. C.; Clark, M. A.; Barrett, E. L. Journal of Bacteriology, Vol. 171, Issue 6 https://doi.org/10.1128/jb.171.6.3008-3015.1989	journal	June 1989
Component A2 of methylcoenzyme M reductase system from Methanobacterium thermoautotrophicum delta H: nucleotide sequence and functional expression by Escherichia coli Kuhner, C. H.; Lindenbach, B. D.; Wolfe, R. S. Journal of Bacteriology, Vol. 175, Issue 10 https://doi.org/10.1128/jb.175.10.3195-3203.1993	journal	May 1993
Genome-Resolved Metagenomic Analysis Reveals Roles for Candidate Phyla and Other Microbial Community Members in Biogeochemical Transformations in Oil Reservoirs Hu, Ping; Tom, Lauren; Singh, Andrea mBio, Vol. 7, Issue 1 https://doi.org/10.1128/mBio.01669-15	journal	January 2016
Sulfide Generation by Dominant Halanaerobium Microorganisms in Hydraulically Fractured Shales Booker, Anne E.; Borton, Mikayla A.; Daly, Rebecca A. mSphere, Vol. 2, Issue 4 https://doi.org/10.1128/mSphereDirect.00257-17	journal	July 2017
The Microbial Community and Functional Potential in the Midland Basin Reveal a Community Dominated by Both Thiosulfate and Sulfate-Reducing Microorganisms Tinker, Kara; Lipus, Daniel; Gardiner, James Microbiology Spectrum, Vol. 10, Issue 4 https://doi.org/10.1128/spectrum.00049-22	journal	August 2022
Hydraulic Fracturing: Paving the Way for a Sustainable Future? Chen, Jiangang; Al-Wadei, Mohammed H.; Kennedy, Rebekah C. M. Journal of Environmental and Public Health, Vol. 2014 https://doi.org/10.1155/2014/656824	journal	January 2014
SpoVG is an important regulator of sporulation and affects biofilm formation by regulating Spo0A transcription in Bacillus cereus 0–9 Huang, Qiubin; Zhang, Zhen; Liu, Qing BMC Microbiology, Vol. 21, Issue 1 https://doi.org/10.1186/s12866-021-02239-6	journal	June 2021
Genomic and transcriptomic insights into methanogenesis potential of novel methanogens from mangrove sediments Zhang, Cui-Jing; Pan, Jie; Liu, Yang Microbiome, Vol. 8, Issue 1 https://doi.org/10.1186/s40168-020-00876-z	journal	June 2020
The Functional Potential of Microbial Communities in Hydraulic Fracturing Source Water and Produced Water from Natural Gas Extraction Characterized by Metagenomic Sequencing Mohan, Arvind Murali; Bibby, Kyle J.; Lipus, Daniel PLoS ONE, Vol. 9, Issue 10 https://doi.org/10.1371/journal.pone.0107682	journal	October 2014
Water Management Challenges Associated with the Production of Shale Gas by Hydraulic Fracturing Gregory, K. B.; Vidic, R. D.; Dzombak, D. A. Elements, Vol. 7, Issue 3 https://doi.org/10.2113/gselements.7.3.181	journal	June 2011
Formation Damage and Impact on Gas Flow Caused by Biofilms Growing Within Proppant Packing Used in Hydraulic Fracturing Bottero, Simona; Picioreanu, Cristian; Enzien, Michael V. SPE International Symposium and Exhibition on Formation Damage Control https://doi.org/10.2118/128066-MS	conference	April 2013
Microbial diversity and methanogenic activity of Antrim Shale formation waters from recently fractured wells Wuchter, Cornelia; Banning, Erin; Mincer, Tracy J. Frontiers in Microbiology, Vol. 4 https://doi.org/10.3389/fmicb.2013.00367	journal	January 2013
Roles of thermophilic thiosulfate-reducing bacteria and methanogenic archaea in the biocorrosion of oil pipelines Liang, Renxing; Grizzle, Robert S.; Duncan, Kathleen E. Frontiers in Microbiology, Vol. 5 https://doi.org/10.3389/fmicb.2014.00089	journal	March 2014
Metabolic Capability of a Predominant Halanaerobium sp. in Hydraulically Fractured Gas Wells and Its Implication in Pipeline Corrosion Liang, Renxing; Davidova, Irene A.; Marks, Christopher R. Frontiers in Microbiology, Vol. 7 https://doi.org/10.3389/fmicb.2016.00988	journal	June 2016
Environmental Drivers of Differences in Microbial Community Structure in Crude Oil Reservoirs across a Methanogenic Gradient Shelton, Jenna L.; Akob, Denise M.; McIntosh, Jennifer C. Frontiers in Microbiology, Vol. 7 https://doi.org/10.3389/fmicb.2016.01535	journal	September 2016
Identification of Persistent Sulfidogenic Bacteria in Shale Gas Produced Waters Cliffe, Lisa; Nixon, Sophie L.; Daly, Rebecca A. Frontiers in Microbiology, Vol. 11 https://doi.org/10.3389/fmicb.2020.00286	journal	February 2020
Out From the Shadows – Resolution of the Taxonomy of the Family Cryomorphaceae Bowman, John P. Frontiers in Microbiology, Vol. 11 https://doi.org/10.3389/fmicb.2020.00795	journal	May 2020
Geochemistry and Microbiology Predict Environmental Niches With Conditions Favoring Potential Microbial Activity in the Bakken Shale Tinker, Kara; Gardiner, James; Lipus, Daniel Frontiers in Microbiology, Vol. 11 https://doi.org/10.3389/fmicb.2020.01781	journal	July 2020
Methyl-Coenzyme M Reductase and Its Post-translational Modifications Chen, Hao; Gan, Qinglei; Fan, Chenguang Frontiers in Microbiology, Vol. 11 https://doi.org/10.3389/fmicb.2020.578356	journal	October 2020
Metagenomic Investigation of a Low Diversity, High Salinity Offshore Oil Reservoir Scheffer, Gabrielle; Hubert, Casey R. J.; Enning, Dennis R. Microorganisms, Vol. 9, Issue 11 https://doi.org/10.3390/microorganisms9112266	journal	October 2021
Role of Thiosulfate in the Corrosion of Steels: A Review Choudhary, Lokesh; Macdonald, Digby D.; Alfantazi, Akram CORROSION, Vol. 71, Issue 9 https://doi.org/10.5006/1709	journal	September 2015
MetaBAT, an efficient tool for accurately reconstructing single genomes from complex microbial communities Kang, Dongwan D.; Froula, Jeff; Egan, Rob PeerJ, Vol. 3 https://doi.org/10.7717/peerj.1165	journal	January 2015

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Title: Metagenome-assembled genomes provide insight into the metabolic potential during early production of Hydraulic Fracturing Test Site 2 in the Delaware Basin

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