Molecular mechanisms and environmental adaptations of flagellar loss and biofilm growth of Rhodanobacter under environmental stress

Chen, Mingfei; Trotter, Valentine V.; Walian, Peter J.; Chen, Yan; Lopez, Romario; Lui, Lauren M.; Nielsen, Torben N.; Malana, Ria Gracielle; Thorgersen, Michael P.; Hendrickson, Andrew J.; Carion, Héloïse; Deutschbauer, Adam M.; Petzold, Christopher J.; Smith, Heidi J.; Arkin, Adam P.; Adams, Michael W.; Fields, Matthew W.; Chakraborty, Romy

doi:10.1093/ismejo/wrae151

Title: Molecular mechanisms and environmental adaptations of flagellar loss and biofilm growth of Rhodanobacter under environmental stress

Journal Article · Thu Aug 08 00:00:00 UTC 2024 · The ISME Journal

DOI: https://doi.org/10.1093/ismejo/wrae151 · OSTI ID:2460472

Chen, Mingfei ^[1]; Trotter, Valentine V. ^[1]; Walian, Peter J. ^[1]; Chen, Yan ^[1]; Lopez, Romario ^[1]; Lui, Lauren M. ^[1]; Nielsen, Torben N. ^[1]; Malana, Ria Gracielle ^[1]; Thorgersen, Michael P. ^[2]; Hendrickson, Andrew J. ^[1]; Carion, Héloïse ^[1]; Deutschbauer, Adam M. ^[3]; Petzold, Christopher J. ^[1]; Smith, Heidi J. ^[4]; Arkin, Adam P. ^[3]; Adams, Michael W. ^[2]; Fields, Matthew W. ^[4]; Chakraborty, Romy ^[1]

Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)
Univ. of Georgia, Athens, GA (United States)
Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States); University of California, Berkeley, CA (United States)
Montana State Univ., Bozeman, MT (United States)

Biofilms aid bacterial adhesion to surfaces via direct and indirect mechanisms, and formation of biofilms is considered as an important strategy for adaptation and survival in suboptimal environmental conditions. However, the molecular underpinnings of biofilm formation in subsurface sediment/groundwater ecosystems where microorganisms often experience fluctuations in nutrient input, pH, and nitrate or metal concentrations are underexplored. Here, we examined biofilm formation under different nutrient, pH, metal, and nitrate regimens of 16 Rhodanobacter strains isolated from subsurface groundwater wells spanning diverse levels of pH (3.5 to 5) and nitrates (13.7 to 146 mM). Eight Rhodanobacter strains demonstrated significant biofilm growth under low pH, suggesting adaptations for survival and growth at low pH. Biofilms were intensified under aluminum stress, particularly in strains possessing fewer genetic traits associated with biofilm formation, findings warranting further investigation. Through random barcode transposon-site sequencing (RB-TnSeq), proteomics, use of specific mutants, and transmission electron microscopy analysis, we discovered flagellar loss under aluminum stress, indicating a potential relationship between motility, metal tolerance, and biofilm growth. Comparative genomic analyses revealed the absence of flagella and chemotaxis genes and the presence of a putative type VI secretion system in the highly biofilm-forming strain FW021-MT20. In this study we identified genetic determinants associated with biofilm growth under metal stress in a predominant environmental genus, Rhodanobacter, and identified traits aiding survival and adaptation to contaminated subsurface environments.

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Research Organization:: Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Biological and Environmental Research (BER)

Grant/Contract Number:: AC02-05CH11231

OSTI ID:: 2460472

Journal Information:: The ISME Journal, Journal Name: The ISME Journal Journal Issue: 1 Vol. 18; ISSN 1751-7362

Publisher:: Nature Publishing GroupCopyright Statement

Country of Publication:: United States

Language:: English

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59 BASIC BIOLOGICAL SCIENCES
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Title: Molecular mechanisms and environmental adaptations of flagellar loss and biofilm growth of Rhodanobacter under environmental stress

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