High phenotypic and genotypic plasticity among strains of the mushroom-forming fungus Schizophyllum commune

Marian, Ioana M.; Valdes, Ivan D.; Hayes, Richard D.; LaButti, Kurt; Duffy, Kecia; Chovatia, Mansi; Johnson, Jenifer; Ng, Vivian; Lugones, Luis G.; Wösten, Han A.B.; Grigoriev, Igor V.; Ohm, Robin A.

doi:10.1016/j.fgb.2024.103913

Title: High phenotypic and genotypic plasticity among strains of the mushroom-forming fungus Schizophyllum commune

Journal Article · Sun Jul 14 00:00:00 UTC 2024 · Fungal Genetics and Biology

DOI: https://doi.org/10.1016/j.fgb.2024.103913 · OSTI ID:2440835

Marian, Ioana M. ^[1]; Valdes, Ivan D. ^[1]; Hayes, Richard D. ^[2]; LaButti, Kurt ^[2]; Duffy, Kecia ^[2]; Chovatia, Mansi ^[2]; Johnson, Jenifer ^[2]; Ng, Vivian ^[2]; Lugones, Luis G. ^[1]; Wösten, Han A.B. ^[1]; Grigoriev, Igor V. ^[3]; Ohm, Robin A. ^[1]

Utrecht University (Netherlands)
USDOE Joint Genome Institute (JGI), Berkeley, CA (United States); Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)
USDOE Joint Genome Institute (JGI), Berkeley, CA (United States); Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States); University of California, Berkeley, CA (United States)

Schizophyllum commune is a mushroom-forming fungus notable for its distinctive fruiting bodies with split gills. It is used as a model organism to study mushroom development, lignocellulose degradation and mating type loci. It is a hypervariable species with considerable genetic and phenotypic diversity between the strains. In this study, we systematically phenotyped 16 dikaryotic strains for aspects of mushroom development and 18 monokaryotic strains for lignocellulose degradation. There was considerable heterogeneity among the strains regarding these phenotypes. The majority of the strains developed mushrooms with varying morphologies, although some strains only grew vegetatively under the tested conditions. Growth on various carbon sources showed strain-specific profiles. The genomes of seven monokaryotic strains were sequenced and analyzed together with six previously published genome sequences. Moreover, the related species Schizophyllum fasciatum was sequenced. Although there was considerable genetic variation between the genome assemblies, the genes related to mushroom formation and lignocellulose degradation were well conserved. These sequenced genomes, in combination with the high phenotypic diversity, will provide a solid basis for functional genomics analyses of the strains of S. commune.

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Research Organization:: Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Basic Energy Sciences (BES). Scientific User Facilities (SUF); USDOE Office of Science (SC), Biological and Environmental Research (BER). Biological Systems Science (BSS)

Grant/Contract Number:: AC02-05CH11231

OSTI ID:: 2440835

Journal Information:: Fungal Genetics and Biology, Journal Name: Fungal Genetics and Biology Vol. 173; ISSN 1087-1845

Publisher:: ElsevierCopyright Statement

Country of Publication:: United States

Language:: English

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