Feedstock variability impacts the bioconversion of sugar and lignin streams derived from corn stover by  Clostridium tyrobutyricum and engineered Pseudomonas putida

Ruhl, Ilona A.; Nelson, Robert S.; Katahira, Rui; Kruger, Jacob S.; Chen, Xiaowen; Haugen, Stefan J.; Ingraham, Morgan A.; Woodworth, Sean P.; Alt, Hannah; Ramirez, Kelsey J.; Peterson, Darren J.; Ding, Ling; Laible, Philip D.; Linger, Jeffrey G.; Salvachúa, Davinia

doi:10.1111/1751-7915.70006

Title: Feedstock variability impacts the bioconversion of sugar and lignin streams derived from corn stover by Clostridium tyrobutyricum and engineered Pseudomonas putida

Journal Article · Thu Sep 05 00:00:00 UTC 2024 · Microbial Biotechnology (Online)

DOI: https://doi.org/10.1111/1751-7915.70006 · OSTI ID:2439808

Ruhl, Ilona A. ^[1]; Nelson, Robert S. ^[1]; Katahira, Rui ^[1]; Kruger, Jacob S. ^[1]; Chen, Xiaowen ^[1]; Haugen, Stefan J. ^[1]; Ingraham, Morgan A. ^[1];

^[1]; Alt, Hannah ^[1]; Ramirez, Kelsey J. ^[1]; Peterson, Darren J. ^[1]; Ding, Ling ^[2]; Laible, Philip D. ^[3]; Linger, Jeffrey G. ^[1];

^[1]

Bioenergy Sciences and Technology Directorate, National Renewable Energy Laboratory Golden Colorado USA
Energy and Environmental Science and Technology, Idaho National Laboratory Idaho Falls Idaho USA
Biosciences Division, Argonne National Laboratory Lemont Illinois USA

Abstract Feedstock variability represents a challenge in lignocellulosic biorefineries, as it can influence both lignocellulose deconstruction and microbial conversion processes for biofuels and biochemicals production. The impact of feedstock variability on microbial performance remains underexplored, and predictive tools for microbial behaviour are needed to mitigate risks in biorefinery scale‐up. Here, twelve batches of corn stover were deconstructed via deacetylation, mechanical refining, and enzymatic hydrolysis to generate lignin‐rich and sugar streams. These batches and their derived streams were characterised to identify their chemical components, and the streams were used as substrates for producing muconate and butyrate by engineered Pseudomonas putida and wildtype Clostridium tyrobutyricum , respectively. Bacterial performance (growth, product titers, yields, and productivities) differed among the batches, but no strong correlations were identified between feedstock composition and performance. To provide metabolic insights into the origin of these differences, we evaluated the effect of twenty‐three isolated chemical components on these microbes, including three components in relevant bioprocess settings in bioreactors, and we found that growth‐inhibitory concentrations were outside the ranges observed in the streams. Overall, this study generates a foundational dataset on P. putida and C. tyrobutyricum performance to enable future predictive models and underscores their resilience in effectively converting fluctuating lignocellulose‐derived streams into bioproducts.

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Research Organization:: Argonne National Laboratory (ANL), Argonne, IL (United States); Idaho National Laboratory (INL), Idaho Falls, ID (United States); National Renewable Energy Laboratory (NREL), Golden, CO (United States)

Sponsoring Organization:: USDOE; USDOE Office of Energy Efficiency and Renewable Energy (EERE), Transportation Office. Bioenergy Technologies Office

Grant/Contract Number:: AC02-06CH11357; AC07-05ID14517; AC36-08GO28308

OSTI ID:: 2439808

Report Number(s):: INL/JOU--24-79321-Rev000; NREL/JA--2800-90312; e70006

Journal Information:: Microbial Biotechnology (Online), Journal Name: Microbial Biotechnology (Online) Journal Issue: 9 Vol. 17; ISSN 1751-7915

Publisher:: Wiley-BlackwellCopyright Statement

Country of Publication:: United Kingdom

Language:: English

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Related Subjects

09 BIOMASS FUELS
37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY
agricultural waste
aromatic compound
bacteria
biorefinery
butyrate
clostridium
corn stover
enzymes
fermentation
lignin
lignocellulose
muconate
pretreatment
pseudomonas
sugar
sustainable aviation fuels
valorization

Title: Feedstock variability impacts the bioconversion of sugar and lignin streams derived from corn stover by Clostridium tyrobutyricum and engineered Pseudomonas putida

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